Novel cationic tannin/glycosaminoglycan-based polyelectrolyte multilayers promote stem cells adhesion and proliferation

Câmara, Paulo C.F. da; Balaban, Rosângela de Carvalho; Hedayati, Mohammadhasan; Martins, Alessandro F.; Kipper, Matt J.

doi:10.1039/c9ra03903a

Cited by 37 publications

(27 citation statements)

References 61 publications

(113 reference statements)

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“…[ 11,23,24 ] Natural polymers are attractive materials for biomedical applications, as they inherently present chemistries similar to the ECM, and they may have the capability to increase cell migration and proliferation. [ 4,22,25,26 ]…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

Madruga

Balaban

Popat

et al. 2020

Macromolecular Bioscience

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This study presents a new type of biocompatible nanofiber based on poly(vinyl alcohol) (PVA) and carboxymethyl‐kappa‐carrageenan (CMKC) blends, produced with no generation of hazardous waste. The nanofibers are produced by electrospinning using PVA:CMKC blends with ratios of 1:0, 1:0.25, 1:0.4, 1:0.5, and 1:0.75 (w/w PVA:CMKC) in aqueous solution, followed by thermal crosslinking. The diameter of the fibers is in the nanometer scale and below 300 nm. Fourier transform infrared spectroscopy shows the presence of the carboxyl and sulfate groups in all the fibers with CMKC. The nanofibers from water‐soluble polymers are stabilized by thermal crosslinking. The incorporation of CMKC improves cytocompatibility, biodegradability, cell growth, and cell adhesion, compared to PVA nanofibers. Furthermore, the incorporation of CMKC modulates phenotype of human adipose‐derived stem cells (ADSCs). PVA/CMKC nanofibers enhance ADSC response to osteogenic differentiation signals and are therefore good candidates for application in tissue engineering to support stem cells.

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Section: Introductionmentioning

confidence: 99%

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

Madruga

Balaban

Popat

et al. 2020

Macromolecular Bioscience

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“…Surface wettability is an essential factor in the characterization of multilayers because the wetting properties control protein adsorption, and subsequently cell attachment [ 42 ]. In addition, wetting properties can be also used to follow the process of multilayer formation because of differences in wetting properties of the different polyelectrolytes [ 17 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

Engineering osteogenic microenvironments by combination of multilayers from collagen type I and chondroitin sulfate with novel cationic liposomes

Barrera

Hause

Menzel

et al. 2020

Materials Today Bio

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Cationic liposomes composed of a novel lipid (N-{6-amino-1-[N-(9Z) -octadec9-enylamino] -1-oxohexan-(2S) -2-yl} –N’- {2- [N, N-bis(2-aminoethyl) amino] ethyl} -2-hexadecylpropandiamide) (OO4) and dioleoylphosphatidylethanolamine (DOPE) possess high amounts of amino groups and are promising systems for lipofection. Moreover, these cationic liposomes can also be used as a polycationic entity in multilayer formation using layer-by-layer technique (LbL), which is a method to fabricate surface coatings by alternating adsorption of polyanions and polycations. Since liposomes are suitable for endocytosis by or fusion with cells, controlled release of their cargo on site is possible. Here, a polyelectrolyte multilayer (PEM) system was designed of chondroitin sulfate (CS) and collagen type I (Col I) by LbL technique with OO4/DOPE liposomes embedded in the terminal layers to create an osteogenic microenvironment. Both, the composition of PEM and cargo of the liposomes were used to promote osteogenic differentiation of C2C12 myoblasts as in vitro model. The internalization of cargo-loaded liposomes from the PEM into C2C12 cells was studied using lipophilic (Rhodamine-DOPE conjugate) and hydrophilic (Texas Red–labeled dextran) model compounds. Besides, the use of Col I and CS should mimic the extracellular matrix of bone for future applications such as bone replacement therapies. Physicochemical studies of PEM were done to characterize the layer growth, thickness, and topography. The adhesion of myoblast cells was also evaluated whereby the benefit of a cover layer of CS and finally Col I above the liposome layer was demonstrated. As proof of concept, OO4/DOPE liposomes were loaded with dexamethasone, a compound that can induce osteogenic differentiation. A successful induction of osteogenic differentiation of C2C12 cells with the novel designed liposome-loaded PEM system was shown. These findings indicate that designed OH4/DOPE loaded PEMs have a high potential to be used as drug delivery or transfection system for implant coating in the field of bone regeneration and other applications.

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“…The PEMs were then constructed via layer‐by‐layer approach to modify the surface chemistry. PEMs were constructed using five layers to change the chemistry of NT surface, without modifying the topography (da Câmara et al, ). SEM and XPS results show that PEMs do not coat the nanotube surface (Figures and 3a).…”

Section: Discussionmentioning

confidence: 99%

“…One promising technique recently investigated is the layer‐by‐layer (LbL) deposition of polyelectrolyte multilayers (PEMs). LbL assembly is used to change the surface chemistry of materials by alternately adsorbing/depositing polycationic and polyanionic layers onto a solid substrate (da Câmara et al, ). PEMs can be easily and reproducibly prepared, to achieve control over coating thickness and surface chemistry, without the use of hazardous organic solvents (Almodóvar, Place, Gogolski, Erickson, & Kipper, ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers

Sabino

Kauk

Madruga

et al. 2020

J Biomedical Materials Res

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Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices. For decades, titanium-based implants have been largely used for different medical applications. However, titanium can neither suppress blood coagulation, nor prevent bacterial infections. To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed. The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements. To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.The antibacterial activity was investigated against Gram-negative P. aeruginosa and Gram-positive S. aureus. Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM. The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation. Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation. The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.

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Novel cationic tannin/glycosaminoglycan-based polyelectrolyte multilayers promote stem cells adhesion and proliferation

Abstract: Condensed tannin is a biologically derived polycation that can be combined with glycosaminoglycans (chondroitin sulfate and heparin) to prepare polyelectrolyte multilayers that promote stem cell adhesion and proliferation.

Cited by 37 publications

References 61 publications

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

Engineering osteogenic microenvironments by combination of multilayers from collagen type I and chondroitin sulfate with novel cationic liposomes

Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers

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