Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers

Abstract

Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices. For decades, titanium-based implants have been largely used for different medical applications. However, titanium can neither suppress blood coagulation, nor prevent bacterial infections. To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed. The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements. To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.The antibacterial activity was investigated against Gram-negative P. aeruginosa and Gram-positive S. aureus. Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM. The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation. Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation. The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl‐Kappa‐Carrageenan Produced by a Green Process

“…[ 38 ] Based on these criteria, CMKC could be an excellent candidate material to be used as a substitute for KC in biomaterial fabrication for tissue engineering and regenerative medicine. [ 39,40 ]…”

“…[38] Based on these criteria, CMKC could be an excellent candidate material to be used as a substitute for KC in biomaterial fabrication for tissue engineering and regenerative medicine. [39,40] In this work, we produced biocompatible crosslinked nanofibers of poly(vinyl alcohol)/carboxymethyl-kappacarrageenan (PVA/CMKC) through electrospinning of an aqueous blend solution of both polymers, followed by thermal crosslinking of the nanofibers. An insoluble biomaterial was obtained through a green process, without the use of hazardous solvents or generation of hazardous waste.…”

Strategies for Improving Antimicrobial Properties of Stainless Steel

“…Many different approaches have been attempted to thwart microbial corrosion [ 100 ], to counteract bacterial adhesion [ 101 ] or to confer bactericidal or antibacterial properties to the material surfaces [ 102 , 103 ], while also improving biological responses, such as hemocompatibility [ 101 , 103 ]. Future research will undoubtedly be aimed at optimizing the biological performances of materials, such as SS, by modifying their surfaces.…”

Simple application of adipose-derived stem cell-derived extracellular vesicles coating enhances cytocompatibility and osteoinductivity of titanium implant

“…With recent progress, many methods have been used to graft biocompatible molecules onto Ti implants such as heparin chemistry, chitosan functional coating, layer-by-layer method and so on [ 29 , 31 ]. Fibronectin is a matrix protein that can be simply anchored onto Ti implant by passive physisorption.…”