Novel Biomaterials for Tissue Engineering 2018

Stratakis, Emmanuel

doi:10.3390/ijms19123960

Cited by 26 publications

(17 citation statements)

References 26 publications

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“…They can be easily shaped in desirable way and biodegraded in controllable fashion. Merging them into composites with varieties of other materials offers even more possibilities to customize them according to individual needs [9,10]. Ability to incorporate specific biomolecules and drugs can further increase their therapeutic efficacy in vivo.…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.

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Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Trcin

Zdraveva

Dolenec³

et al. 2020

Polymers

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“…Hydrogels are ideally suited to mimic key features of tissues for applications as in vitro platforms for cell culture, tissue engineering, and drug testing. Hydrogels are derived from natural and synthetic sources and are composed of macromolecules (polymers, proteins, glycans) that interact non-covalently or are crosslinked to form an insoluble macrostructure (Edgar et al, 2016;Ruedinger et al, 2015;Stratakis, 2018;Xu et al, 2012). When applied toward an in vitro model or an in vivo implant, hydrogels are associated with proteins, and most often, proteins are located within the molecular structure of the hydrogel.…”

Section: Confinementmentioning

confidence: 99%

Protein folding and assembly in confined environments: Implications for protein aggregation in hydrogels and tissues

Simpson

Good

Leach

2020

Biotechnology Advances

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In the biological milieu of a cell, soluble crowding molecules and rigid confined environments strongly influence whether the protein is properly folded, intrinsically disordered proteins assemble into distinct phases, or a denatured or aggregated protein species is favored. Such crowding and confinement factors act to exclude solvent volume from the protein molecules, resulting in an increased local protein concentration and decreased protein entropy. A protein's structure is inherently tied to its function. Examples of processes where crowding and confinement may strongly influence protein function include transmembrane protein dimerization, enzymatic activity, assembly of supramolecular structures (e.g., microtubules), nuclear condensates containing transcriptional machinery, protein aggregation in the contexts of disease and protein therapeutics. Historically, most protein structures have been determined from pure, dilute protein solutions or pure crystals. However, these are not the environments in which these proteins function. Thus, there has been an increased emphasis on analyzing protein structure and dynamics in more "in vivo-like" environments. Complex in vitro models using hydrogel scaffolds to study proteins may better mimic features of the in vivo environment. Therefore, analytical techniques need to be optimized for real-time analysis of proteins within hydrogel scaffolds.

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“…Moreover, from the application point of view, Qasim et al [ 91 ] showed that the electrospinning of chitosan and its composite formulations for creating fibers in combination with other natural polymers is actively working in tissue engineering. It shows that the favorable properties and biocompatibility of chitosan electrospun composite biomaterials can be used for a wide range of applications [ 92 , 93 ].…”

Section: Bioadhesive Biomaterials’ Biomedical Applicationsmentioning

confidence: 99%

Polymer Based Bioadhesive Biomaterials for Medical Application—A Perspective of Redefining Healthcare System Management

Saha

Sáha

et al. 2020

Polymers

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This article deliberates about the importance of polymer-based bioadhesive biomaterials’ medical application in healthcare and in redefining healthcare management. Nowadays, the application of bioadhesion in the health sector is one of the great interests for various researchers, due to recent advances in their formulation development. Actually, this area of study is considered as an active multidisciplinary research approach, where engineers, scientists (including chemists, physicists, biologists, and medical experts), material producers and manufacturers combine their knowledge in order to provide better healthcare. Moreover, while discussing the implications of value-based healthcare, it is necessary to mention that health comprises three main domains, namely, physical, mental, and social health, which not only prioritize the quality healthcare, but also enable us to measure the outcomes of medical interventions. In addition, this conceptual article provides an understanding of the consequences of the natural or synthetic polymer-based bioadhesion of biomaterials, and its significance for redefining healthcare management as a novel approach. Furthermore, the research assumptions highlight that the quality healthcare concept has recently become a burning topic, wherein healthcare service providers, private research institutes, government authorities, public service boards, associations and academics have taken the initiative to restructure the healthcare system to create value for patients and increase their satisfaction, and lead ultimately to a healthier society.

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Novel Biomaterials for Tissue Engineering 2018

Abstract: The concept of regenerating tissues, with properties and functions that mimic natural tissues, has attracted significant attention in recent years. [...]

Cited by 26 publications

References 26 publications

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Poly(ε-caprolactone) Titanium Dioxide and Cefuroxime Antimicrobial Scaffolds for Cultivation of Human Limbal Stem Cells

Protein folding and assembly in confined environments: Implications for protein aggregation in hydrogels and tissues

Polymer Based Bioadhesive Biomaterials for Medical Application—A Perspective of Redefining Healthcare System Management

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