Novel biological function of sialic acid (<i>N</i>‐acetylneuraminic acid) as a hydrogen peroxide scavenger

Iijima, Ryosuke; Takahashi, Hideyo; Namme, Rie; Ikegami, Shiro; Yamazaki, Masatoshi

doi:10.1016/s0014-5793(04)00164-4

Cited by 115 publications

(96 citation statements)

References 11 publications

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“…Release of free monomeric sialic acid when it is cleaved from the sugar chain can detoxify H 2 O 2 (22). This reaction reduces the dangerous oxygen radicals, as H 2 O 2 and NANA are converted into H 2 O and nontoxic carboxylic acid (21,22).…”

Section: Discussionmentioning

confidence: 99%

“…Sialidase activity using the Amplex Red neuraminidase kit was tested in the total cell fraction (T), the extracellular fraction (Ex), and cell lysate (CL). * , P Ͻ 0.001. against oxidative damage (22). The relative significance of the P. gingivalis sialidase and O-sialoglycoprotease in oxidative stress resistance was evaluated by exposure of the isogenic defective mutants to hydrogen peroxide.…”

Section: Vol 79 2011 Sialidase Activity In Porphyromonas Gingivalismentioning

confidence: 99%

“…Bacterial sialidases have been considered virulence factors in many pathogenic organisms, such as Corynebacterium diphtheriae, Vibrio cholerae, Streptococcus pneumoniae, and group B streptococci, which colonize mucosal surfaces (8). They have been shown to be involved in infection and tissue destruction (57), peroxide scavenging during oxidative stress (22), and the modulation of host innate immunity (55). Furthermore, production of sialidase may be a critical factor in the provision of free sialic acid, a fermentable carbohydrate source for bacterial proliferation (8,20).…”

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confidence: 99%

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Sialidase and Sialoglycoproteases Can Modulate Virulence in Porphyromonas gingivalis

et al. 2011

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The Porphyromonas gingivalis recombinant VimA can interact with the gingipains and several other proteins, including a sialidase. Sialylation can be involved in protein maturation; however, its role in virulence regulation in P. gingivalis is unknown. The three sialidase-related proteins in P. gingivalis showed the characteristic sialidase Asp signature motif (SXDXGXTW) and other unique domains. To evaluate the roles of the associated genes, randomly chosen P. gingivalis isogenic mutants created by allelic exchange and designated FLL401 (PG0778::ermF), FLL402 (PG1724::ermF), and FLL403 (PG0352::ermF-ermAM) were characterized. Similar to the wild-type strain, FLL402 and FLL403 displayed a black-pigmented phenotype in contrast to FLL401, which was not black pigmented. Sialidase activity in P. gingivalis FLL401 was reduced by approximately 70% in comparison to those in FLL402 and FLL403, which were reduced by approximately 42% and 5%, respectively. Although there were no changes in the expression of the gingipain genes, their activities were reduced by 60 to 90% in all the isogenic mutants compared to that for the wild type. Immunoreactive bands representing the catalytic domains for RgpA, RgpB, and Kgp were present in FLL402 and FLL403 but were missing in FLL401. While adhesion was decreased, the capacity for invasion of epithelial cells by the isogenic mutants was increased by 11 to 16% over that of the wild-type strain. Isogenic mutants defective in PG0778 and PG0352 were more sensitive to hydrogen peroxide than the wild type. Taken together, these results suggest that the P. gingivalis sialidase activity may be involved in regulating gingipain activity and other virulence factors and may be important in the pathogenesis of this organism.

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Section: Discussionmentioning

confidence: 99%

Section: Vol 79 2011 Sialidase Activity In Porphyromonas Gingivalismentioning

confidence: 99%

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confidence: 99%

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Sialidase and Sialoglycoproteases Can Modulate Virulence in Porphyromonas gingivalis

et al. 2011

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“…Presently, studies investigating the correlation between serum total sialic acid and the severity of atherosclerosis have presented conflicting results. It is noteworthy that recent studies have demonstrated that sialic acid could scavenge ROS 13, 14, 15 and may be applied as therapeutic option in some diseases 16, 17, 18.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and cardiovascular protective effects of quercetin 7‐O‐sialic acid

Tian

Liu

Qin

et al. 2016

J Cellular Molecular Medi

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Oxidative stress and inflammation play important roles in the pathogenesis of cardiovascular disease (CVD). Oxidative stress‐induced desialylation is considered to be a primary step in atherogenic modification, and therefore, the attenuation of oxidative stress and/or inflammatory reactions may ameliorate CVD. In this study, quercetin 7‐O‐sialic acid (QA) was synthesized aiming to put together the cardiovascular protective effect of quercetin and the recently reported anti‐oxidant and anti‐atherosclerosis functions of N‐acetylneuraminic acid. The biological efficacy of QA was evaluated in vitro in various cellular models. The results demonstrated that 50 μM QA could effectively protect human umbilical vein endothelial cells (HUVEC, EA.hy926) against hydrogen peroxide‐ or oxidized low‐density lipoprotein‐induced oxidative damage by reducing the production of reactive oxygen species. QA attenuated hydrogen peroxide‐induced desialylation of HUVEC and lipoproteins. QA decreased lipopolysaccharide‐induced secretion of tumour necrosis factor‐α (TNF‐α) and monocyte chemoattractant protein‐1 (MCP‐1), and it significantly reduced the expression of intercellular adhesion molecule‐1, vascular cell adhesion molecule‐1, TNF‐α and MCP‐1. Furthermore, QA effectively promoted cholesterol efflux from Raw 264.7 macrophages to apolipoprotein A‐1 and high‐density lipoprotein by up‐regulating ATP‐binding cassette transporter A1 and G1, respectively. Results indicated that the novel compound QA exhibited a better capacity than quercetin for anti‐oxidation, anti‐inflammation, cholesterol efflux promotion and biomolecule protection against desialylation and therefore could be a candidate compound for the prevention or treatment of CVD.

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“…Furthemore, it is a prcedictor of several systemic disorders, Cardiovascular disease, rheumatoid arthritis, and diabetes (Iijima et al, 2004;Baenke et al, 2013). Gangliosides are well known for their role in the mediation of several diseascs such as tetanus, botulism and infuenza by acting as cellular receptors for invading pathogens (Stringou et al, 1992;Ariga et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis Value of Membrane Glycolipids Biochemistry Index in Intracranial and Gastrointestinal Tumors

Lv²,

Mei³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The diagnostic value of membrane glycolipid biochemistry index, the lipid-bound sialic acid (LSA) and total sialic acid (TSA) in cerebrospinal fluid (CSF) was evaluated in 30 intracranial and 65 gastrointestinal tumors. The plasma LSA, TSA and red cell membrane sialic acid (R-SA) in were determined according to the method of Sevenmerhulm. Our results showed that the levels of LSA and TSA in CSF of intracranial tumor patients was higher than that of normal group(p<0.01). The concentration of TSA and LSA in patients with malignant glioma was higher than that of benign meningioma patients(P<0.01). No significance was found between intracranial halmatoma patients and normal control group for levels of membrane glycolipids (p>0.05). Results also found that the plasma LSA, TSA and R-SA of gastric carcinoma were significantly higher than those of control group (p<0.05); while no significant difference was found in the plasma LSA, TSA and R-SA levels between chronic gastritis, gastrohelcoma and normal control group (p>0.05). Plasma LSA, TSA and R-SA levels of gastric carcinoma patient were significantly higher than those of chronic gastritis patients and gastrohelcoma patients(p<0.05). It was also found that plasma LSA, TSA and R-SA contents were significantly higher in large intestine carcinoma patients than in benign in stestine tumor patients (p<0.05) while no significant difference was found between intestine benign tumor and normal control group (p>0.05). The levels of LSA, TSA and R-SA were obviously higher in the patients with metastasis than in the ones without (p<0.05.) The membrane glycolipid biochemistry index LSA and TSA in CSF are sensive markers for diagnosing intracranial tumors. For gastrointestinal malignant tumors the plasma LSA TSA and red blood cell membrane SA may be considered as auxiliary indicators for diagnosis. They can be used for distinguishing benign from malignant tumors.

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Novel biological function of sialic acid (N‐acetylneuraminic acid) as a hydrogen peroxide scavenger

Cited by 115 publications

References 11 publications

Sialidase and Sialoglycoproteases Can Modulate Virulence in Porphyromonas gingivalis

Sialidase and Sialoglycoproteases Can Modulate Virulence in Porphyromonas gingivalis

Synthesis and cardiovascular protective effects of quercetin 7‐O‐sialic acid

Diagnosis Value of Membrane Glycolipids Biochemistry Index in Intracranial and Gastrointestinal Tumors

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