Novel assay procedures for the measurement of <i>α</i>‐amylase in weather‐damaged wheat

Cornaggia, Claudio; Ivory, Ruth; Mangan, David; McCleary, Barry V.

doi:10.1002/jsfa.7103

Cited by 16 publications

(9 citation statements)

References 10 publications

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“…The Megazyme α‐amylase activity level of 0.23 U/g may indicate that although considered “sound,” the Diva control had some low level of LMA. Cornaggia, Ivory, Mangan, and McCleary () noted that a sample with Falling Number of 373 s had an α‐amylase activity of 0.047 U/g. Nevertheless, the higher levels of α‐amylase in the PHS and PHS/LMA samples were clearly detected successfully despite the kit's intended use on human pancreatic α‐amylase.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of commercial α‐amylase enzyme‐linked immunosorbent assay (ELISA) test kits for wheat

Kiszonas

Morris

2018

Cereal Chem

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Background and objectives There are few methods in wheat to directly measure α‐amylase, which is the result of preharvest sprouting (PHS) and late maturity α‐amylase (LMA). Both of these conditions are associated with reduced wheat and flour quality. Those methods that do exist for measuring α‐amylase in wheat are based on enzyme activity. The objective of this study was to evaluate commercially available enzyme‐linked immunosorbent assay (ELISA) kits on sound grain, sprouted grain, and grain that had both PHS and LMA. Findings Three kits were evaluated; human AMY1 and AMY2, and a “plant α‐amylase” kit. The wheat samples were also assayed for α‐amylase activity using the Megazyme SD α‐amylase method. All three ELISA kits functioned very well based on calibration curves (R2 > .99). Of the three, the AMY1 and plant α‐amylase kits appeared to show no useful detection of wheat α‐amylase. The AMY2 kit, however, detected a 2.7‐fold increase in α‐amylase protein in the PHS and PHS/LMA grain lots, similar to a 2.7‐fold difference in activity. Conclusions It appears that the particular human AMY2A ELISA kit, and perhaps others, can detect α‐amylase in wheat and may be a useful alternative technology to enzyme kits. These kits may also be useful in predicting PHS and LMA potential damage. Significance and novelty Thus far, α‐amylase studies have focused on enzyme kits, whereas this study examined three commercially available ELISA kits to measure α‐amylase content in sound, sprouted, and PHS/LMA grain. Both PHS and LMA are highly detrimental to grain and result in low prices paid to growers.

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Section: Resultsmentioning

confidence: 99%

Evaluation of commercial α‐amylase enzyme‐linked immunosorbent assay (ELISA) test kits for wheat

Kiszonas

Morris

2018

Cereal Chem

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“…We used 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as the fluorescence labeling reagent to simultaneously detect two triterpenic acids in Swertia species (Li, You, et al, 2011a;Li, Zhang, et al, 2011b). Owing to the lack of strong chromophores in triterpenic acid molecules, the fluorescence derivatization could enhance the sensitivity and selectivity (Cornaggia, Ivory, Mangan, & McCleary, 2015;Li, Kong, et al, 2015a;Li, Liu, et al, 2015b). The novel method showed the advantages of the lower detection limits at 1.10 and 1.30 ng mL −1 than the other mentioned methods.…”

Section: Introductionmentioning

confidence: 99%

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

You

Zhao

et al. 2017

Biomedical Chromatography

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Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of triterpenic acid bioavailability. Inspired by that, a versatile method, which overcomes the difficulty of the reported methods, has been first developed in this study. The novel method using 2-[12-benzo[b]acridin-5- (12H)-yl]-acetohydrazide (BAAH) as the fluorescence labeling reagent coupled with high-performance liquid chromatography with fluorescence detection was first developed for the study of triterpenic acid bioavailability. Furthermore, the labeling conditions have been optimized in order to achieve the best fluorescence labeling yield. Under the optimal conditions, the quantitative linear range of analytes was 2-1000 ng mL , and the correlation coefficients were >0.9998. The detection limits for all triterpenic acid derivatives were achieved within the range of 0.28-0.29 ng mL . The proposed method was successfully applied to the study of triterpenic acid bioavailability with excellent applicability and good reproducibility.

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“…Thus it is necessary to develop an effective, easy and sensitive method for simultaneously screening acidic micronutrients in infant foodstuffs. With the advantages of high selectivity, sensitivity and applicability, fluorescent detection was widely used in the trace determination . In the present study we tried, for the first time, to use fluorescence labeling strategy combined with HPLC–fluorescence detection (FLD) for simultaneous determination of free acidic micronutrients including biotin (VB 7 ), nicotinic acid (VB 3 ), linoleic acid (LNA), linolenic acid (LA), arachidonic acid (AA), EPA and DHA in infant foods.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive screening of some acidic micronutrients in infant foods by HPLC with fluorescent detector

Kong

Fan

et al. 2015

J. Sci. Food Agric.

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Novel assay procedures for the measurement of α‐amylase in weather‐damaged wheat

Abstract: The assay using Amylase SD is completely specific for α-amylase. The use of the Amylase SD assay represents a sensitive and valid alternative to the traditionally used Falling Number values for the assessment of sprout damage in wheat samples.

Cited by 16 publications

References 10 publications

Evaluation of commercial α‐amylase enzyme‐linked immunosorbent assay (ELISA) test kits for wheat

Evaluation of commercial α‐amylase enzyme‐linked immunosorbent assay (ELISA) test kits for wheat

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Rapid and sensitive screening of some acidic micronutrients in infant foods by HPLC with fluorescent detector

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