Novel Approaches to Minimizing Mycotoxin Contamination

Rodrı́guez, Mar; Núñez, Félix

doi:10.3390/toxins12040216

Cited by 14 publications

(14 citation statements)

References 15 publications

(15 reference statements)

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“…Many researchers have studied the management of mycotoxigenic fungi in food and feed using biological treatments such as probiotics microorganisms by assimilation or degradation approaches ( Kim et al, 2017 ). Indeed, the biological approaches are increasingly explored due to their higher effectiveness and safety for humans ( Jaibangyang et al, 2020 ; Rodríguez and Núñez, 2020 ). Our probiotic candidate L. brevis MYSN105 and its CFS both reduced significantly the growth of mycotoxigenic fungi, with findings from this study are in good accordance with previous investigations ( Nagaraja et al, 2016 ; Deepthi et al, 2017 ; Plocková et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

et al. 2021

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The use of probiotics and antifungal capabilities of the lactic acid bacteria (LAB) isolated from different niches is a strategy to prepare functional cultures and biopreservatives for food/feed industries. In the present study, LAB strains isolated from an Indian traditional fermented food, Pozha, were evaluated for their probiotic properties and biocontrol potential. A total of 20 LAB isolates were selected from Pozha samples collected aseptically and screened for their antagonistic activity against Fusarium verticillioides. Among the bioactive isolates, Lacticaseibacillus brevis MYSN105 showed the highest antifungal activity in vitro, causing some morphological alterations such as damaged mycelia and deformed conidia. Cell-free supernatant (CFS) from L. brevis MYSN105 at 16% concentration effectively reduced the mycelial biomass to 0.369 g compared to 1.938 g in control. Likewise, the conidial germination was inhibited to 20.12%, and the seed treatment using CFS induced a reduction of spore count to 4.1 × 106 spores/ml compared to 1.1 × 109 spores/ml for untreated seeds. The internal transcribed spacer (ITS) copy number of F. verticillioides decreased to 5.73 × 107 and 9.026 × 107 by L. brevis MYSN105 and CFS treatment, respectively, compared to 8.94 × 1010 in control. The L. brevis MYSN105 showed high tolerance to in vitro gastrointestinal conditions and exhibited high adhesive abilities to intestinal epithelial cell lines. The comparative genome analysis demonstrated specific secondary metabolite region coding for bacteriocin and T3PKS (type III polyketide synthase) possibly related to survival and antimicrobial activity in the gut environment. Our results suggest that L. brevis MYSN105 has promising probiotic features and could be potentially used for developing biological control formulations to minimize F. verticillioides contamination and improve food safety measures.

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Section: Discussionmentioning

confidence: 99%

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

et al. 2021

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“…However, indiscriminate and frequent use of strobilurin (QoI) fungicides has led to the emergence of pathogenic strains resistant to this group of fungicides [ 15 , 16 , 17 ]. In addition, extensive use of these fungicides can cause serious damage to human health and animals, and can disturb the natural ecosystem [ 18 , 19 ]. Moreover, traditional breeding strategies take a longer time to develop resistant varieties, which often break down under field conditions after a few years due to the development of new pathogen races [ 15 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused by Magnaporthe oryzae Triticum

Paul

Chakraborty

Rahman

et al. 2022

JoF

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The application of chemical pesticides to protect agricultural crops from pests and diseases is discouraged due to their harmful effects on humans and the environment. Therefore, alternative approaches for crop protection through microbial or microbe-originated pesticides have been gaining momentum. Wheat blast is a destructive fungal disease caused by the Magnaporthe oryzae Triticum (MoT) pathotype, which poses a serious threat to global food security. Screening of secondary metabolites against MoT revealed that antimycin A isolated from a marine Streptomyces sp. had a significant inhibitory effect on mycelial growth in vitro. This study aimed to investigate the inhibitory effects of antimycin A on some critical life stages of MoT and evaluate the efficacy of wheat blast disease control using this natural product. A bioassay indicated that antimycin A suppressed mycelial growth (62.90%), conidiogenesis (100%), germination of conidia (42%), and the formation of appressoria in the germinated conidia (100%) of MoT at a 10 µg/mL concentration. Antimycin A suppressed MoT in a dose-dependent manner with a minimum inhibitory concentration of 0.005 μg/disk. If germinated, antimycin A induced abnormal germ tubes (4.8%) and suppressed the formation of appressoria. Interestingly, the application of antimycin A significantly suppressed wheat blast disease in both the seedling (100%) and heading stages (76.33%) of wheat at a 10 µg/mL concentration, supporting the results from in vitro study. This is the first report on the inhibition of mycelial growth, conidiogenesis, conidia germination, and detrimental morphological alterations in germinated conidia, and the suppression of wheat blast disease caused by a Triticum pathotype of M. Oryzae by antimycin A. Further study is required to unravel the precise mode of action of this promising natural compound for considering it as a biopesticide to combat wheat blast.

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“…P. expansum that invades wounded apples, causing bleu mold decay, is the most common postharvest pathogen in apples, by affecting the apple quality (soft rot of the whole fruit, localized dark spots on the fruit) in addition to the production of a mycotoxin PAT (Lončarić et al, 2021). For that reason, special attention was directed to the research of mycotoxin‐degrading methods as promising approaches to control its toxic effects (Rodríguez & Núñez, 2020). To this end, here, we developed a screening method based on the patulin enzyme‐linked immunosorbent assay (ELISA) test kit, which is widely used in the fruit industry, since patulin is the major toxin produced by Penicillium species on apples and in apple products such as juice, cider, and compost (Zhong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antifungal potential of two synthetic vanadium (IV) oxalate compounds to control blue mold of oranges and apples under storage conditions

Essghaier

Smiri

Sehimi

et al. 2022

Food Processing Preservation

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Novel Approaches to Minimizing Mycotoxin Contamination

Abstract: Contamination of foods and agricultural commodities by various types of toxigenic fungi is a concerning issue for human and animal health [...]

Cited by 14 publications

References 15 publications

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

Probiotic and Antifungal Attributes of Levilactobacillus brevis MYSN105, Isolated From an Indian Traditional Fermented Food Pozha

Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused by Magnaporthe oryzae Triticum

Antifungal potential of two synthetic vanadium (IV) oxalate compounds to control blue mold of oranges and apples under storage conditions

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