Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused by Magnaporthe oryzae Triticum

Paul, Sanjoy; Chakraborty, Moutoshi; Rahman, M.; Gupta, Dipali Rani; Mahmud, Nur Uddin; Rahat, Abdullah Al Mahbub; Sarker, Aniruddha; Hannan, Md. Abdul; Rahman, Mujibur; Akanda, Abdul Mannan; Ahmed, Jalal Uddin; Islam, Tofazzal

doi:10.3390/jof8060618

Cited by 17 publications

(15 citation statements)

References 83 publications

(111 reference statements)

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“…Wheat seeds cv. BARI Gom-24 (Prodip) was surface sterilized by following the protocol described by Paul et al [38]. Fifteen seeds were sown in a plastic pot (12 cm × 7.5 cm) containing sterilized field soil amended with NPK fertilizer and grown for 15 days, maintaining 12/12 h light dark alternations and 65% humidity.…”

Section: Growing Of Seedlingsmentioning

confidence: 99%

“…Sterilized water was sprayed on inoculated seedlings every day at 4.0 pm to maintain high humidity. Disease development was recorded at 7 d after inoculation, and each treatment was replicated six times [72,73].…”

Section: In Vivo Assay For Biocontrol Activity Of Bacteriamentioning

confidence: 99%

“…Previous studies suggest that the probiotic bacteria can protect rice plants from blast fungus [27] and bacterial species under the genera of Serratia, Pseudomonas [28,29], Streptomyces [30,31], Paraburkholderia [32], and Bacillus [15,33,34]) have been reported as effective antagonists for biological control of rice blast fungus [6,35]. There are only a few reports describing that the pure compounds from some bacteria can suppress the growth of MoT in vitro [1,36,37,38,39]. However, no reports have been published on the biological control of wheat blast by seed endophytic bacteria.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Probiotic Bacillus Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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Plant diseases are among the major factors affecting plant productivity. Biological control of plant diseases is preferred over chemical control as it is environment-friendly, cost-effective, and sustainable. Among many microbes capable of providing biological control of plant diseases, probiotic Bacillus species are most promising as they can survive in adverse conditions, provide plants with a wide range of benefits including protection from phytopathogens. Wheat blast caused by Magnaporthe oryzae Triticum pathotype (MoT) has emerged as a potential threat to global wheat production. Due to unreliability of fungicides and limited cultivar resistance, we aimed to screen and identify potential antagonist bacteria collected from internal tissues of rice and wheat seeds to determine their in vitro and in vivo inhibitory effects against MoT. Dual culture and seedling assays were performed to evaluate the efficacy of probiotic bacteria. Out of 170 bacterial isolates, three bacteria (BTS-3, BTS-4, and BTLK6A) were screened as potential antagonists against MoT in vitro. Artificial inoculation at the seedling stage showed that the isolates BTS-4, BTS–3, and BTLK6A reduced 89, 88, and 85% of wheat blast disease severity, respectively, compared to mock-inoculated control. The bacterial isolates were identified as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) through genome phylogeny. The whole genome sequence of these three bacterial strains decoded a number of orthologs to intrinsic genes of antimicrobial peptides, antioxidant defense enzymes, cell wall degrading enzymes, compounds involved in the induction of systemic resistance (ISR) in host plants, and volatile compounds to make them promising biologicals to control MoT in wheat. Combined data of in vitro and in vivo along with genome analysis suggest that Bacillus spp. suppress the destructive wheat blast disease likely through antibiosis and ISR in the host plants. Further field evaluation and characterization of antimicrobial compounds are needed for a better understanding of the mode of action and practical recommendation of these bacteria for wheat blast control in the farmers’ fields.

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Section: Growing Of Seedlingsmentioning

confidence: 99%

Section: In Vivo Assay For Biocontrol Activity Of Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Probiotic Bacillus Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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“…Direct suppression of target fungi by BCAs can be achieved by the production of antibiotics, volatile organic compounds (VOCs), or other bioactive secondary metabolites [21] that have an antagonistic effect on pathogens [19]. Inhibitory activity and biocontrol potential of some pure microbial non-volatile secondary metabolites against the wheat blast fungus MoT have been reported recently [24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast

Surovy¹,

Rahman²,

Rostás³

et al. 2023

Preprint

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Magnaporthe oryzae Triticum (MoT) pathotype is the causal agent of wheat blast, which has caused significant economic losses and threatens wheat production in South America, Asia and Africa. Three bacterial strains from rice and wheat seeds (B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) were used to explore the antifungal effects of volatile organic compounds (VOCs) of Bacillus spp. as a potential biocontrol mechanism against MoT. All bacterial treatments significantly inhibited both mycelial growth and sporulation of MoT in vitro. We found that this inhibition was caused by Bacillus VOCs in a dose-dependent manner. In addition, biocontrol assays using detached wheat leaves infected with MoT showed reduced leaf lesions and sporulation compared to the untreated control. VOCs from B. velezensis BTS-4 alone or a consortium (mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) treatments consistently suppressed MoT in vitro and in vivo. Compared to the untreated control, VOCs from BTS-4 and Bacillus consortium reduced MoT lesions in vivo by 85% and 81.25%, respectively. A total of thirty-nine VOCs (from 9 different VOC groups) from 4 Bacillus treatments were identified by gas chromatography-mass spectrometry (GC-MS), of which 11 were produced in all Bacillus treatments. Alcohols, fatty acids, ketones, aldehydes, and S-containing compounds were detected in all four bacterial treatments. In vitro assays using pure VOCs revealed that hexanoic acid, 2-methylbutanoic acid, and phenylethyl alcohol are potential VOCs emitted by Bacillus spp. that are suppressive for MoT. The minimum inhibitory concentrations for MoT sporulation were 250 mM for phenylethyl alcohol and 500 mM for 2-methylbutanoic acid and hexanoic acid. Therefore, our results indicate that VOCs from Bacillus spp. are effective compounds to suppress the growth and sporulation of MoT. Understanding the MoT sporulation reduction mechanisms by Bacillus VOCs may provide novel options to manage the further spread of wheat blast by spores.

show abstract

“…Direct suppression of target fungi by BCAs can be achieved by the production of antibiotics, volatile organic compounds (VOCs), or other bioactive secondary metabolites [ 21 ] that have an antagonistic effect on pathogens [ 19 ]. The inhibitory activity and biocontrol potential of some pure microbial non-volatile secondary metabolites against the wheat blast fungus MoT have been reported recently [ 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast

et al. 2023

Self Cite

View full text Add to dashboard Cite

The Magnaporthe oryzae Triticum (MoT) pathotype is the causal agent of wheat blast, which has caused significant economic losses and threatens wheat production in South America, Asia, and Africa. Three bacterial strains from rice and wheat seeds (B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) were used to explore the antifungal effects of volatile organic compounds (VOCs) of Bacillus spp. as a potential biocontrol mechanism against MoT. All bacterial treatments significantly inhibited both the mycelial growth and sporulation of MoT in vitro. We found that this inhibition was caused by Bacillus VOCs in a dose-dependent manner. In addition, biocontrol assays using detached wheat leaves infected with MoT showed reduced leaf lesions and sporulation compared to the untreated control. VOCs from B. velezensis BTS-4 alone or a consortium (mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) of treatments consistently suppressed MoT in vitro and in vivo. Compared to the untreated control, VOCs from BTS-4 and the Bacillus consortium reduced MoT lesions in vivo by 85% and 81.25%, respectively. A total of thirty-nine VOCs (from nine different VOC groups) from four Bacillus treatments were identified by gas chromatography–mass spectrometry (GC–MS), of which 11 were produced in all Bacillus treatments. Alcohols, fatty acids, ketones, aldehydes, and S-containing compounds were detected in all four bacterial treatments. In vitro assays using pure VOCs revealed that hexanoic acid, 2-methylbutanoic acid, and phenylethyl alcohol are potential VOCs emitted by Bacillus spp. that are suppressive for MoT. The minimum inhibitory concentrations for MoT sporulation were 250 mM for phenylethyl alcohol and 500 mM for 2-methylbutanoic acid and hexanoic acid. Therefore, our results indicate that VOCs from Bacillus spp. are effective compounds to suppress the growth and sporulation of MoT. Understanding the MoT sporulation reduction mechanisms exerted by Bacillus VOCs may provide novel options to manage the further spread of wheat blast by spores.

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Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused by Magnaporthe oryzae Triticum

Cited by 17 publications

References 83 publications

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Suppressive Effects of Volatile Compounds from <em>Bacillus </em>spp. on <em>Magnaporthe oryzae Triticum</em> (MoT) Pathotype, Causal Agent of Wheat Blast

Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast

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