Novel Approach to Track the Lifecycle of Inflammation from Chemokine Expression to Inflammatory Proteins in Sweat Using Electrochemical Biosensor

Jagannath, Badrinath; Pali, Madhavi; Lin, Kai‐Chun; Sankhala, Devangsingh; Naraghi, Pejman; Muthukumar, Sriram; Prasad, Shalini

doi:10.1002/admt.202101356

Cited by 21 publications

(15 citation statements)

References 36 publications

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“…The sensor platform has been described in detail elsewhere [ 15 , 28 , 31 ]. Briefly, the sensor platform was designed with four zinc oxide (ZnO)-coated electrode pairs on a porous substrate.…”

Section: Resultsmentioning

confidence: 99%

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A Proof-of-Concept Electrochemical Skin Sensor for Simultaneous Measurement of Glial Fibrillary Acidic Protein (GFAP) and Interleukin-6 (IL-6) for Management of Traumatic Brain Injuries

et al. 2022

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This work demonstrates the use of a noninvasive, sweat-based dual biomarker electrochemical sensor for continuous, prognostic monitoring of a Traumatic Brain Injury (TBI) with the aim of enhancing patient outcomes and reducing the time to treatment after injury. A multiplexed SWEATSENSER was used for noninvasive continuous monitoring of glial fibrillary acidic protein (GFAP) and Interleukin-6 (IL-6) in a human sweat analog and in human sweat. Electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) were used to measure the sensor response. The assay chemistry was characterized using Fourier Transform Infrared Spectroscopy (FTIR). The SWEATSENSER was able to detect GFAP and IL-6 in sweat over a dynamic range of 3 log orders for GFAP and 2 log orders for IL-6. The limit of detection (LOD) for GFAP detection in the sweat analog was estimated to be 14 pg/mL using EIS and the LOD for IL-6 was estimated to be 10 pg/mL using EIS. An interference study was performed where the specific signal was significantly higher than the non-specific signal. Finally, the SWEATSENSER was able to distinguish between GFAP and IL-6 in simulated conditions of a TBI in human sweat. This work demonstrates the first proof-of-feasibility of a multiplexed TBI marker combined with cytokine and inflammatory marker detection in passively expressed sweat in a wearable form-factor that can be utilized toward better management of TBIs. This is the first step toward demonstrating a noninvasive enabling technology that can enable baseline tracking of an inflammatory response.

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Section: Resultsmentioning

confidence: 99%

“…The description of the sweat sensor has been provided in detail previously [ 15 , 28 , 31 ]. Briefly, the sensor device had a sensor strip and a wearable reader.…”

Section: Methodsmentioning

confidence: 99%

A Proof-of-Concept Electrochemical Skin Sensor for Simultaneous Measurement of Glial Fibrillary Acidic Protein (GFAP) and Interleukin-6 (IL-6) for Management of Traumatic Brain Injuries

et al. 2022

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“…In addition, the detection of genetic material and structural proteins is directly related to the viral load, considered a predictor of the severity and progression of the disease, presenting biomarkers that predict disease prognosis, progression, and severity. In addition, moving from conventional detection to using (c) different robust commercial detection kits requiring specialized equipment and personnel to (d) a single multiparametric device based on sensitive, specific and portable nanobiosensors [48,49] the most critical states and higher probability of death in patients with higher viral load [110]. Serological tests have also been used to detect SARS-CoV-2 infection, an indirect method that detects antibodies generated by the body of infected people.…”

Section: Biomarkers For Diagnosismentioning

confidence: 99%

“…It must be clarified that they were not interested in specific biomarker detection (only 50%) but in indicating disease severity with biomarkers of inflammation in blood samples, so they did not use bioreceptors in the detection. And finally, Jagannath et al [49] developed an electrochemical biosensor for the detection of inflammatory proteins interferon-inducible protein 10 (IP-10), TNF-related apoptosis-inducing ligand (TRAIL), and CrP in sweat as a strategy for monitoring infections such as COVID-19 in a noninvasive and portable way, achieving LOD of 1 pg/mL (IP-10 and TRAIL) and 0.2 ng/mL (CrP). The biosensor was based on a zinc oxide semiconductor electrode system modified with specific monoclonal antibodies, detecting the biomarkers by EIS.…”

Section: Biosensors For Prognosis and Prediction Of The Course Of Cov...mentioning

confidence: 99%

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Vásquez

Orozco

2022

Anal Bioanal Chem

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As a more efficient and effective way to address disease diagnosis and intervention, cutting-edge technologies, devices, therapeutic approaches, and practices have emerged within the personalized medicine concept depending on the particular patient's biology and the molecular basis of the disease. Personalized medicine is expected to play a pivotal role in assessing disease risk or predicting response to treatment, understanding a person's health status, and, therefore, health care decision-making. This work discusses electrochemical biosensors for monitoring multiparametric biomarkers at different molecular levels and their potential to elucidate the health status of an individual in a personalized manner. In particular, and as an illustration, we discuss several aspects of the infection produced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a current health care concern worldwide. This includes SARS-CoV-2 structure, mechanism of infection, biomarkers, and electrochemical biosensors most commonly explored for diagnostics, prognostics, and potentially assessing the risk of complications in patients in the context of personalized medicine. Finally, some concluding remarks and perspectives hint at the use of electrochemical biosensors in the frame of other cutting-edge converging/emerging technologies toward the inauguration of a new paradigm of personalized medicine. Graphical abstract

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“…Eccrine sweat is an attractive class of biofluid suitable for the noninvasive monitoring of body chemistry. Sweat contains a rich composition of biomarkers relevant to physiological health status including electrolytes (1), metabolites (2)(3)(4), hormones (5,6), proteins (7), and exogenous agents (8). Studies demonstrate the intermittent or continuous assessment of these, and other sweat biomarkers offer time dynamic insight into the metabolic processes of the body relevant to applications ranging from athletic performance (9)(10)(11) to medical diagnostics (2,(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Skin-interfaced microfluidic systems with spatially engineered 3D fluidics for sweat capture and analysis

Hing

Baessler

et al. 2023

Sci. Adv.

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Skin-interfaced wearable systems with integrated microfluidic structures and sensing capabilities offer powerful platforms for monitoring the signals arising from natural physiological processes. This paper introduces a set of strategies, processing approaches, and microfluidic designs that harness recent advances in additive manufacturing [three-dimensional (3D) printing] to establish a unique class of epidermal microfluidic (“epifluidic”) devices. A 3D printed epifluidic platform, called a “sweatainer,” demonstrates the potential of a true 3D design space for microfluidics through the fabrication of fluidic components with previously inaccessible complex architectures. These concepts support integration of colorimetric assays to facilitate in situ biomarker analysis operating in a mode analogous to traditional epifluidic systems. The sweatainer system enables a new mode of sweat collection, termed multidraw, which facilitates the collection of multiple, independent sweat samples for either on-body or external analysis. Field studies of the sweatainer system demonstrate the practical potential of these concepts.

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Novel Approach to Track the Lifecycle of Inflammation from Chemokine Expression to Inflammatory Proteins in Sweat Using Electrochemical Biosensor

Abstract: only be of significant value as an early warning system but also for better evidence-based prognosis and patient-centric therapeutic intervention.Research data are not shared.

Cited by 21 publications

References 36 publications

A Proof-of-Concept Electrochemical Skin Sensor for Simultaneous Measurement of Glial Fibrillary Acidic Protein (GFAP) and Interleukin-6 (IL-6) for Management of Traumatic Brain Injuries

A Proof-of-Concept Electrochemical Skin Sensor for Simultaneous Measurement of Glial Fibrillary Acidic Protein (GFAP) and Interleukin-6 (IL-6) for Management of Traumatic Brain Injuries

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Skin-interfaced microfluidic systems with spatially engineered 3D fluidics for sweat capture and analysis

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