Novel approach to enhancing remineralization of tooth surface by magnetic field exposure

El-Kaliuoby, Mai. I.; Hamouda, Rasha; El‐Khatib, Ahmed M.

doi:10.1680/jsuin.20.00018

Cited by 3 publications

(1 citation statement)

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“…In a different way, exposure to a magnetic field of 1.8 mT for 20 min was adopted to develop the kinetics of dentin/enamel re-mineralization in combination with nano-chitosan gels. The results established the applicability of using a magnetic field as an accelerator for tooth remineralization [22]. In addition to the ability of PEF to decrease the bacteria growth rates it also accelerates the wound healing process [23,24].…”

Section: Introductionmentioning

confidence: 83%

Enhancement of Nano-Biopolymer Antibacterial Activity by Pulsed Electric Fields

2021

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Chronic wounds are commonly colonized with bacteria in a way that prevents full healing process and capacity for repair. Nano-chitosan, a biodegradable and nontoxic biopolymer, has shown bacteriostatic activity against a wide spectrum of bacteria. Effectively, pulsed electromagnetic fields are shown to have both wound healing enhancement and antibacterial activity. This work aimed to combine the use of nano-chitosan and exposure to a pulsed electric field to overcome two common types of infectious bacteria, namely P. aeruginosa and S. aureus. Here, bacteria growing rate, growth kinetics and cell cytotoxicity (levels of lactate dehydrogenase, protein leakage and nucleic acid leakage) were investigated. Our findings confirmed the maximum antibacterial synergistic combination of nano-chitosan and exposure against P. aeruginosa than using each one alone. It is presumed that the exposure has influenced bacteria membrane charge distribution in a manner that allowed more chitosan to anchor the surface and enter inside the cell. Significantly, cell cytotoxicity substantiates high enzymatic levels as a result of cell membrane disintegration. In conclusion, exposure to pulsed electromagnetic fields has a synergistic antibacterial effect against S. aureus and P. aeruginosa with maximum inhibitory effect for the last one. Extensive work should be done to evaluate the combination against different bacteria types to get general conclusive results. The ability of using pulsed electromagnetic fields as a wound healing accelerator and antibacterial cofactor has been proved, but in vivo experimental work in the future to verify the use of such a new combination against infectious wounds and to determine optimum treatment conditions is a must.

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Section: Introductionmentioning

confidence: 83%

Enhancement of Nano-Biopolymer Antibacterial Activity by Pulsed Electric Fields

2021

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Enhancing of teeth remineralization by electric field aid (in vitro study)

Khalil

El‐Khatib

Hamouda

et al. 2023

Bioinspired, Biomimetic and Nanobiomaterials

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One of the major challenges in teeth re-mineralization is the slow rate of mineralization. In this paper, we developed electric field-aided mineralization system for rapidly regenerating teeth-like structure rapidly. The efficacy of application of electric field combined with Nano-chitosan gel, casein phosphopeptide amorphous calcium phosphate gel and their mixture was investigated as re-mineralizing accelerator for artificially demineralized teeth. Teeth samples have been demineralized by using ethylenediaminetetraacetic acid and subsequently subjected to each gel alone and combined with electric field (20 mA- 5 min). The structural analyses of teeth samples were examined by using scanning electron microscope and X-ray diffraction patterns and its hardness was obtained by Vickers micro-hardness test. The results revealed that the addition of chitosan to casein phosphopeptide amorphous calcium phosphate enhanced the re-mineralization of the tooth structure rather than each one individually. Furthermore, the application of electric field has enhanced teeth hardness and showed highest rate of re-mineralization. In conclusion, the proposed technique could be safely used to reduce the consuming time taken in re-mineralization and efficiently it may be used to increase tooth quality. It is suggested to study other biophysical parameters of using electric fields for in vivo applications.

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