Novel Approach for Quantification of Hepatitis C Virus in Liver Cirrhosis Using Real-Time Reverse Transcriptase PCR

Abstract

The results indicate that using Light Cycler 2.0, following the modified technique described, constitutes a reliable method of quantitative detection and localization of HCV in tissue in "serosilent" HCV infection.

HCV-RNA quantification in liver bioptic samples and extrahepatic compartments, using the abbott Real Time HCV assay

“…Several home-made methods, based on reverse transcription-polymerase chain reaction (RT-PCR), transcription-mediated amplification, or branched-chain DNA, are available for the detection of HCV-RNA in both serum and liver (Sakamoto et al, 1994;Dailey et al, 1999;Maudar et al, 2012;Ramirez et al, 2009;Harouaka et al, 2016;Hedegaard et al, 2017;Gambato et al, 2016). However, a commercial validated assay for the HCV RNA quantification in the liver tissue is still missing.…”

Quantitation assay of hepatitis C virus RNA using real-time PCR technique

“…Hepatitis C virus (HCV) is a single stranded RNA virus belonging to the Flaviviridae family, it has been identified as a major pathogen of post transfusion and community transmitted hepatitis [1,2]. It is estimated that 170 million people worldwide are infected with HCV [3,4].…”

“…Patients with HCV infection develop chronic liver disease, in this group, about 30% progress to cirrhosis, and 1-2% develops hepatocellular carcinoma per year [9,10].…”

“…HCV may also contaminate through tissue transplantation, drug usage via injection, sexual intercourse with infected individuals, and used contaminated tools [4,9].…”