Novel applications of multichannel spectroscopy in pharmaceutical analysis

Fell, Anthony F.; Clark, Brian J.; Scott, Hugh P.

doi:10.1016/0731-7085(83)80069-7

Cited by 30 publications

(4 citation statements)

References 53 publications

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“…For selection of the second key row, note that application of eq 3 is identical with determining the cosine between the test vector b and the first key row. Figures 10 and 11 and Tables III and IV present the POSS results before and after application of the SES reordering procedure. The cumulative information curves for the POSS selections are generally inferior to the KSFA results.…”

Section: Methodsmentioning

confidence: 99%

“…For example, deconvolution of overlapped peak profiles (1,2), supression of selected peaks (3), and identification of sample components by computerized searching of a library of stored spectra (4) have been investigated. The monitoring of absorbance ratiograms (5-9) and the collection of spectra throughout the course of a chromatographic run (10,11) have also been described. Additional applications are summarized in a recent review (12).…”

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confidence: 99%

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Selection of wavelengths for absorbance ratio monitoring in liquid chromatography

Fv¹,

Ba²,

Mf³

1987

Anal. Chem.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Selection of wavelengths for absorbance ratio monitoring in liquid chromatography

Fv¹,

Ba²,

Mf³

1987

Anal. Chem.

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“…The application of the spectral suppression method has so far only been described for binary systems. [6][7][8] Therefore, for multiple peak overlap systems in HPLC a slightly different approach is required.…”

Section: Theorymentioning

confidence: 99%

Short papers in Pharmaceutical Analysis

Marr¹,

Horváth²,

Clark³

et al. 1986

Anal. Proc.

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The plant Cutharunthus roseus is an important source of the naturally occurring antineoplastic alkaloids vincristine and vinblastine. The quality control of the extraction process has routinely relied on a two-dimensional thin-layer chromatographic (TLC) densitometric method.' However, the precision obtained by TLC is less than satisfactory. Consequently, an attempt was made to develop a suitable HPLC method.A review of the methods reported in the recent literature revealed that most were unsatisfactory for routine quality control, as they required a complex separatory process or resulted in lengthy retention time~.2-~ However, Gorog er ~1 . ~ proposed an HPLC system, which, although not able to separate completely all of the constituents, was simple, rapid and apparently robust. Preliminary investigations of various packing materials in our laboratories showed that a 10-cm column packed with SAS Hypersil was approximately equivalent to the 25-cm Lichrosorb RP-8 column used in the original method. The optimum eluent composition and the elution order of the different alkaloids were similar for the two columns. It was not possible, however, to separate completely vincristine, ajmalacine and catharanthine from one another using the shorter column, and this observation was consistent with the results from the original 25-cm Lichrosorb RP-8 column reported by Gorog et ul.5In a collaborative study with Professor Goriig and his co-workers, digital methods have been examined to address the problem of deconvoluting overlapping peaks. The present work reports the development of a multi-component suppression system for this purpose that is capable of routine application. The system is based o n a linear photodiode array detector and represents a logical extension of the spectral suppression method originally ExperimentalReagents and Materials A 0.01 M solution of ammonium carbonate (AnalaR grade, BDH Chemicals Ltd., Poole) was prepared with glass distilled water and filtered through a Millipore 0.45 pm filter using an all-glass apparatus. Acetonitrile (HPLC "S" grade, Rathburn Chemicals, Walkerburn) was used as received. The eluent was de-gassed under vacuum in an ultrasonic bath for 15 min. Catharanthine sulphate, vincristine sulphate and ajmalacine were provided by Gedeon Richter Chemical Works (Budapest, Hungary). Standard solutions of each of the analytes alone, in binary and in ternary mixtures were prepared in the HPLC mobile phase. The combination solutions contained vincristine sulphate (84.15 pg ml-l), ajmalacine (89.48 pg ml-1) and catharanthine sulphate (2.14-85.4 pg ml-I ) .

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“…Optical multichannel imaging spectroscopy (18) has recently undergone many technological improvements (19) which have allowed both the silicon vidicon (20, 21) and the linear photodiode array (PDA) (19,(22)(23)(24)(25) to be successfully employed as detectors in conventional scale LC. While vidicon tubes have traditionally been used for fluorometric detection, the more recent linear photodiode arrays, or their intensified counterparts, are rapidly gaining in popularity as UV-visible absorbance detectors.…”

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confidence: 99%