Miniature fluorometric photodiode array detection system for capillary chromatography

Gluckman, Jennifer C.; Shelly, Dennis C.; Novotný, Miloš V.

doi:10.1021/ac00285a012

Cited by 65 publications

(35 citation statements)

References 45 publications

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“…Fluorescence emission provides more selectivity and increased sensitivity compared to UV absorption and RI detection. Further, emission spectra can be utilized to reveal structural information of unknown compounds [148,149], and the ability to measure the packing of the column [150,151].…”

Section: Fluorescence Detectionmentioning

confidence: 99%

“…Most applications deal with laser-induced fluorescence detection or -as mentioned earlier -detection of fluorescence emission in the packing. Exceptions are the work of Gluckman and co-workers [148,149], who used a miniaturized fluorimetric array detector for the detection and identification of large polyaromatic compounds. The utility of fluorescence emission data was represented by comparing mass spectral data and the emission spectra from wellresolved peaks from a separation of neutral polycyclic aromatic compounds extracted from fuel oil.…”

Section: Fluorescence Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Fluorescence Detectionmentioning

confidence: 99%

Section: Fluorescence Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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show abstract

“…It has been employed in many detectors for the transmission of excitation light [26] and the collection of fluorescence [23,25,27]. To obtain a more flexible coupling of light into the separation column, optical fiber was inserted directly into the capillary for multichannel fluorescence detection in capillary chromatography [28,29] and LIF detection in CE [30].…”

Section: -10mentioning

confidence: 99%

Optical fiber light‐emitting diode‐induced fluorescence detection for capillary electrophoresis

2006

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A highly sensitive optical fiber light-emitting diode (LED)-induced fluorescence detector for CE has been constructed and evaluated. In this detector, a violet or blue LED was used as the excitation source and an optical fiber with 40 microm OD was used to transmit the excitation light. The upper end of the fiber was inserted into the separation capillary and was situated right at the detection window. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a cutoff filter before reaching the photomultiplier tube. Output signals were recorded and processed with a computer using in-house written software. The present CE/fluorescence detector deploys a simple and inexpensive optical system that requires only an LED as the light source. Its utility was successfully demonstrated by the separation and determination of amino acids (AAs) labeled with naphthalene-2,3-dicarboxaldehyde (NDA) and FITC. Low detection limits were obtained ranging from 17 to 23 nM for NDA-tagged AAs and 8 to 12 nM for FITC-labeled AAs (S/N=3). By virtue of such valuable features as low cost, convenience, and miniaturization, the presented detection scheme was proven to be attractive for sensitive fluorescence detection in CE.

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“…In order to obtain a more flexible coupling of light into the separation column, a technique for fabricating and employing a 10-cm-wide optical fiber arrayassisted LIF detection for CE has been described [43]. An optical fiber was inserted directly into a capillary for multichannel fluorescence detection in capillary chromatography [44,45] and LIF detection in CE [46].…”

Section: Introductionmentioning

confidence: 99%

In‐column fiber‐optic laser‐induced fluorescence detection for CE

et al. 2007

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A highly sensitive in-column fiber-optic LIF detector for CE has been constructed and evaluated. In this detection system, a 457-nm diode-pumped solid-state blue laser was used as the excitation light source and an optical fiber (40 mum od) was used to transmit the excitation light. One end of the optical fiber was inserted into the separation capillary and was in situ positioned at the detection window. The other end of the fiber was protruded from the capillary to capture the excitation light beam from the blue laser. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a yellow color filter before reaching the photomultiplier tube. The present CE-fluorescence detection is a simple and compact optical system. It reduces the laser scattering effect from the capillary and fiber as compared to the conventional LIF detection for CE. Its utility was successfully demonstrated by the separation and determination of D-penicillamine labeled with naphthalene-2,3-dicarboxaldehyde. The detection limit was 0.8 nM (S/N = 3). The present detection scheme has been proven to be attractive for sensitive fluorescence detection for CE.

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Miniature fluorometric photodiode array detection system for capillary chromatography

Cited by 65 publications

References 45 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Optical fiber light‐emitting diode‐induced fluorescence detection for capillary electrophoresis

In‐column fiber‐optic laser‐induced fluorescence detection for CE

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