Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach

Khairnar, Krishna; Chandekar, Rajshree; Pal, Preeti; Paunikar, Waman

doi:10.1080/21655979.2015.1134066

Cited by 19 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Khainar et al isolated specific bacteriophages against nocardioforms on active sludge process. The activity of three phages applied in a cocktail at the lab scale reactor reduced foam formation [104]. Choi et al isolated a bacteriophage from sewage infecting Sphaerotilus natans, known to cause filamentous bulking in wastewater treatment systems, and their results showed that phage application diminished the sludge volume index and turbidity of the supernatant, indicating that phages can be used in this concern too [105].…”

Section: Bacteriophages In Bioremediationmentioning

confidence: 99%

Phages in Food Industry Biocontrol and Bioremediation

et al. 2021

View full text Add to dashboard Cite

Bacteriophages are ubiquitous in nature and their use is a current promising alternative in biological control. Multidrug resistant (MDR) bacterial strains are present in the livestock industry and phages are attractive candidates to eliminate them and their biofilms. This alternative therapy also reduces the non-desirable effects produced by chemicals on food. The World Health Organization (WHO) estimates that around 420,000 people die due to a foodborne illness annually, suggesting that an improvement in food biocontrol is desirable. This review summarizes relevant studies of phage use in biocontrol focusing on treatments in live animals, plants, surfaces, foods, wastewaters and bioremediation.

show abstract

Section: Bacteriophages In Bioremediationmentioning

confidence: 99%

Phages in Food Industry Biocontrol and Bioremediation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Phage-mediated mortality has the potential to influence the treatment performance of a system through controlling the abundance of key functional groups, leading to their utilisation as a biocontrol strategy to lyse filamentous bacteria that are responsible for bulking in activated sludge [ 16 , 17 ]. In contrast, bacteriophage predation in wastewater systems has been demonstrated to cause the collapse of reactors [ 18 ] and the failure of bacterial biological processes such as phosphorus removal and nitrification [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial predation accelerates granulation and modulates microbial community composition

et al. 2021

View full text Add to dashboard Cite

Background Bacterial communities are responsible for biological nutrient removal and flocculation in engineered systems such as activated floccular sludge. Predators such as bacteriophage and protozoa exert significant predation pressure and cause bacterial mortality within these communities. However, the roles of bacteriophage and protozoan predation in impacting granulation process remain limited. Recent studies hypothesised that protozoa, particularly sessile ciliates, could have an important role in granulation as these ciliates were often observed in high abundance on surfaces of granules. Bacteriophages were hypothesized to contribute to granular stability through bacteriophage-mediated extracellular DNA release by lysing bacterial cells. This current study investigated the bacteriophage and protozoan communities throughout the granulation process. In addition, the importance of protozoan predation during granulation was also determined through chemical killing of protozoa in the floccular sludge. Results Four independent bioreactors seeded with activated floccular sludge were operated for aerobic granulation for 11 weeks. Changes in the phage, protozoa and bacterial communities were characterized throughout the granulation process. The filamentous phage, Inoviridae, increased in abundance at the initiation phase of granulation. However, the abundance shifted towards lytic phages during the maturation phase. In contrast, the abundance and diversity of protozoa decreased initially, possibly due to the reduction in settling time and subsequent washout. Upon the formation of granules, ciliated protozoa from the class Oligohymenophorea were the dominant group of protozoa based on metacommunity analysis. These protozoa had a strong, positive-correlation with the initial formation of compact aggregates prior to granule development. Furthermore, chemical inhibition of these ciliates in the floccular sludge delayed the initiation of granule formation. Analysis of the bacterial communities in the thiram treated sludge demonstrated that the recovery of ‘Candidatus Accumulibacter’ was positively correlated with the formation of compact aggregates and granules. Conclusion Predation by bacteriophage and protozoa were positively correlated with the formation of aerobic granules. Increases in Inoviridae abundance suggested that filamentous phages may promote the structural formation of granules. Initiation of granules formation was delayed due to an absence of protozoa after chemical treatment. The presence of ‘Candidatus Accumulibacter’ was necessary for the formation of granules in the absence of protozoa.

show abstract

“…Bacteriophages are highly abundant in engineered wastewater systems, appear to be active components of activated sludge systems and are able to infect both planktonic and bio lm associated bacterial cells [13][14][15]. Phage-mediated mortality has the potential to in uence the treatment performance of a system through controlling the abundance of key functional groups, leading to their utilisation as a biocontrol strategy to lyse lamentous bacteria that are responsible for bulking in activated sludge [16,17]. In contrast, bacteriophage predation in wastewater systems has also resulted in failures of bacterial biological processes such as phosphorus removal and nitri cation [18,19].…”

Section: Introductionmentioning

confidence: 99%

Microbial Predation Accelerates Granulation and Modulates Microbial Community Composition

Chan

Ismail

Tan

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundBacterial communities are responsible for biological nutrient removal and flocculation in engineered systems such as activated floccular sludge. Predators such as bacteriophage and protozoa exert significant predation pressure and cause bacterial mortality within these communities. However, the roles of bacteriophage and protozoan predation in impacting granulation process remain limited. Recent studies hypothesised that protozoa, particularly sessile ciliates, could have an important role in granulation as these ciliates were often observed in high abundance on surfaces of granules. Bacteriophages were hypothesized to contribute to granular stability through bacteriophage-mediated extracellular DNA release by lysing bacterial cells. This current study investigated the bacteriophage and protozoan communities throughout the granulation process. In addition, the importance of protozoan predation during granulation was also determined through chemical killing of protozoa in the floccular sludge.ResultsFour independent bioreactors seeded with activated floccular sludge were operated for aerobic granulation for 11 weeks. Changes in the phage, protozoa and bacterial communities were characterized throughout the granulation process. The filamentous phage, Inoviridae, increased in abundance at the initiation phase of granulation. However, the abundance shifted towards lytic phages during the maturation phase. In contrast, the abundance and diversity of protozoa decreased initially, possibly due to the reduction in settling time and subsequent washout. Upon the formation of granules, ciliated protozoa from the class Oligohymenophorea were the dominant group of protozoa based on metacommunity analysis. These protozoa had a strong, positive-correlation with the initial formation of compact aggregates prior to granule development. Furthermore, chemical inhibition of these ciliates in the floccular sludge delayed the initiation of granule formation. Analysis of the bacterial communities in the thiram treated sludge demonstrated that the recovery of ‘Candidatus Accmulibacter’ was positively correlated with the formation of compact aggregates and granules.ConclusionPredation by bacteriophage and protozoa were positively correlated with the formation of aerobic granules. Increases in Inoviridae abundance suggested that filamentous phages may promote the structural formation of granules. Initiation of granules formation was delayed due to an absence of protozoa after chemical treatment. The presence of Candidatus Accumulibacter was necessary for the formation of granules in the absence of protozoa.

show abstract

Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach

Cited by 19 publications

References 7 publications

Phages in Food Industry Biocontrol and Bioremediation

Phages in Food Industry Biocontrol and Bioremediation

Microbial predation accelerates granulation and modulates microbial community composition

Microbial Predation Accelerates Granulation and Modulates Microbial Community Composition

Contact Info

Product

Resources

About