Novel Anaplasmataceae agents <i>Candidatus</i> Ehrlichia hydrochoerus and <i>Anaplasma</i> spp. Infecting Capybaras, Brazil

Vieira, Thállitha Samih Wischral Jayme; Collere, Flávia Carolina Meira; Ferrari, Larissa Dantas Roeder; Baggio, Rafael Antunes; Lange, Rogério Ribas; Ferrari, Marcos Vinícius; Duque, Juan Carlos; Sanches, Gustavo Seron; Pereira, Nathalia Assis; Aguiar, Daniel Moura de; Labruna, Marcelo B.; Vieira, Rafael Felipe da Costa

doi:10.3201/eid2802.210705

Cited by 11 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…'Candidatus Ehrlichia pampeana' was detected in Haemaphysalis juxtakochi and in gray Brocket Deer (Mazama gouazoubira) from Uruguay using three loci (16S rRNA, dsb, and groEL) [56]. 'Candidatus Ehrlichia hydrochoerus' was detected in blood samples from capybaras (Hydrochoerus hydrochaeris) from Brazil, using four loci (16S rRNA, dsb, groEL and sodB) [57]. In the present study, we were able to perform the molecular characterization using six different molecular targets, including a large fragment of 16S rRNA, dsb, sodB, groEL, gltA genes and the 23S-5S intergenic space region (ITS).…”

Section: Discussionmentioning

confidence: 99%

Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

et al. 2022

View full text Add to dashboard Cite

The Anaplasmataceae family encompasses obligate intracellular α-proteobacteria of human and veterinary medicine importance. This study performed multi-locus sequencing to characterize Ehrlichia and Anaplasma in coati’s blood samples in Midwestern Brazil. Twenty-five samples (25/165—15.1%) were positive in the screening PCR based on the dsb gene of Ehrlichia spp. and were characterized using 16S rRNA, sodB, groEL, and gltA genes and the 23S-5S intergenic space region (ITS). Phylogenetic analyses based on all six molecular markers positioned the sequences into a new clade, with a common origin of Ehrlichia ruminantium. Haplotype analyses of 16S RNA sequences revealed the presence of two distinct Ehrlichia genotypes. Six samples (6/165, 3.6%) were positive in the screening nPCR for the 16S rRNA gene of Anaplasma spp. and were submitted to an additional PCR targeting the ITS for molecular characterization. Phylogenetic analyses based on both 16S rRNA gene and ITS positioned the Anaplasma sp. detected in the present study in a large clade with other Anaplasma sp. previously detected in ticks and wild animals and in a clade with ‘Candidatus Anaplasma brasiliensis’, respectively. Based on distinct molecular markers, the present work described a putative novel Anaplasmataceae agent, namely ‘Candidatus Ehrlichia dumleri’, and Anaplasma sp. closely related to the previously described ‘Candidatus Anaplasma brasiliensis’.

show abstract

Section: Discussionmentioning

confidence: 99%

Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The remaining 16SrRNA-positive samples amplified only the groEL gene. The obtained sequences (952–1109 bp) showed 99.22% to 99.40% identity to " Candidatus Ehrlichia hydrochoerus" (MW816535), which was previously reported to infect capybaras in Brazil 18 . Phylogenetic analysis using these groEL sequences placed the detected ehrlichial agent, hereinafter referred to as " Ca.…”

Section: Resultsmentioning

confidence: 60%

“…The genus Ehrlichia comprises six formally recognized tick-transmitted species: Ehrlichia canis , Ehrlichia muris , E. chaffeensis , Ehrlichia ewingii , Ehrlichia minasensis , and Ehrlichia ruminantium . Additionally, several different strains of putative novel Ehrlichia species have been molecularly detected recently, although their taxonomic positions are still not clearly defined 13 – 18 .…”

Section: Introductionmentioning

confidence: 99%

Capybara density and climatic factors as modulators of Ehrlichia prevalence in questing ticks in the Iberá wetlands, Argentina

Eberhardt

Manzoli

Fernández

et al. 2023

Sci Rep

View full text Add to dashboard Cite

We evaluated the presence of Ehrlichia spp. in unfed capybara ticks, Amblyomma dubitatum, and explored its association with capybaras density, ticks density and environmental variables. We observed that in the Iberá wetlands ecoregion A. dubitatum is infected by "Candidatus Ehrlichia hydrochoerus” and in a lesser extent with an Ehrlichia species closely related to Ehrlichia chaffeensis. The frequency of "Ca. Ehrlichia hydrochoerus" presence in A. dubitatum was not associated with vector abundance, but the probability of finding "Ca. Ehrlichia hydrochoerus"-infected ticks increased when the density of capybaras was low two months before. We hypothesize that when the density of capybaras decreases, A. dubitatum immature stages may seek out alternative hosts one of which could exhibit high realized reservoir competence for "Ca. Ehrlichia hydrochoerus", leading to an increased prevalence of this ehrlichiae in questing A. dubitatum. High minimum temperatures and high cumulative rainfall in the time period previous to tick collection (15 to 60 days) were positively correlated with the prevalence of "Ca. Ehrlichia hydrochoerus" infection in A. dubitatum. Our results suggest that a combination of factors (both biological and abiotic) could raise the risk of human exposure to tick-borne Ehrlichia in the Iberá wetlands ecoregion.

show abstract

“…romitii , displays a substantial genetic dissimilarity to Ca . Ehrlichia hydrochoerus, a recently proposed species found in Brazilian capybaras 36 (96.11% pairwise nucleotide identities; GenBank MW785879 and MW785880). This diversity pattern suggests that significant differences in the nature of infections can exist among animals of the same vertebrate species originating from distinct regions of South America.…”

Section: Resultsmentioning

confidence: 99%

Detection of Anaplasma and Ehrlichia bacteria in humans, wildlife, and ticks in the Amazon rainforest

Buysse,

Koual,

Binetruy

et al. 2024

Nat Commun

View full text Add to dashboard Cite

Tick-borne bacteria of the genera Ehrlichia and Anaplasma cause several emerging human infectious diseases worldwide. In this study, we conduct an extensive survey for Ehrlichia and Anaplasma infections in the rainforests of the Amazon biome of French Guiana. Through molecular genetics and metagenomics reconstruction, we observe a high indigenous biodiversity of infections circulating among humans, wildlife, and ticks inhabiting these ecosystems. Molecular typing identifies these infections as highly endemic, with a majority of new strains and putative species specific to French Guiana. They are detected in unusual rainforest wild animals, suggesting they have distinctive sylvatic transmission cycles. They also present potential health hazards, as revealed by the detection of Candidatus Anaplasma sparouinense in human red blood cells and that of a new close relative of the human pathogen Ehrlichia ewingii, Candidatus Ehrlichia cajennense, in the tick species that most frequently bite humans in South America. The genome assembly of three new putative species obtained from human, sloth, and tick metagenomes further reveals the presence of major homologs of Ehrlichia and Anaplasma virulence factors. These observations converge to classify health hazards associated with Ehrlichia and Anaplasma infections in the Amazon biome as distinct from those in the Northern Hemisphere.

show abstract

Novel Anaplasmataceae agents Candidatus Ehrlichia hydrochoerus and Anaplasma spp. Infecting Capybaras, Brazil

Cited by 11 publications

References 11 publications

Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

Capybara density and climatic factors as modulators of Ehrlichia prevalence in questing ticks in the Iberá wetlands, Argentina

Detection of Anaplasma and Ehrlichia bacteria in humans, wildlife, and ticks in the Amazon rainforest

Contact Info

Product

Resources

About