Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

Perles, Lívia; Herrera, Heitor Miraglia; Barreto, Wanessa Teixeira Gomes; Macedo, Gabriel Carvalho de; Calchi, Ana Cláudia; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.3390/microorganisms10122379

Cited by 8 publications

(7 citation statements)

References 56 publications

(74 reference statements)

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“…Six sequences (439-519 bp) were obtained and showed 99–100% identity (ID), 89–97% query-coverage (QC) and 0.0 E-value (EV) with Anaplasma sp. sequences previously detected in wild Nasua nasua from Mato Grosso do Sul State 20 , 21 (GenBank accession numbers KY499184, KY499186-87, KY499192-95); and 100% ID, 88% QC and 0.0 EV with Anaplasma sp. detected in a rodent 35 .…”

Section: Resultsmentioning

confidence: 97%

“…In the USA, raccoons ( Procyon lotor ) play a role as reservoirs for Anaplasma phagocytophilum , a zoonotic agent 39 , 41 , 42 . When it comes to coatis, Sousa et al 21 detected an Ehrlichia 16S rDNA genotype phylogenetically related to multiple Ehrlichia spp. previously detected in ticks and wild rodents from Brazil.…”

Section: Discussionmentioning

confidence: 99%

“…chaffeensis, and Anaplasma 16S rRNA genotypes closely related to A. bovis and A. phagocytophilum on coatis in the Pantanal wetlands, state of Mato Grosso do Sul 20 , Collere et al 13 failed to sequence the detected agents. Recently, Perles et al 21 described ‘ Candidatus Ehrlichia dumleri’, a putative novel Anaplasmataceae agent, and Anaplasma sp. closely related to ‘C andidatus Anaplasma brasiliensis’ in blood samples from coatis in peri-urbanized forested areas in central-western Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Perles

Moraes

Silva

et al. 2023

Sci Rep

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The present study aimed to detect molecularly the presence of co-infections by vector-borne agents (VBA) in ring-tailed coatis’ (Nasua nasua) blood samples from Iguaçu National Park (INP), southern Brazil, and assess the phylogenetic positioning of the detected agents. DNA blood samples were submitted to molecular screening and characterization for Anaplasmataceae agents, Piroplasmids, Hepatozoon sp., hemotropic mycoplasmas, and Bartonella spp. In total, 42 (85.7%) coatis were positive for hemotropic Mycoplasma sp., 12 (24.5%) for Bartonella machadoae, 7 (14.3%) for Anaplasma sp. closely related to ‘Candidatus Anaplasma brasiliensis’, and 3 (6%) for Hepatozoon procyonis. The most prevalent co-infections observed was from bacterial VBA: while 18.3% were co-infected by hemotropic Mycoplasma sp. and Bartonella sp., 12.2% were co-infected by Anaplasma sp. and hemotropic Mycoplasma sp. Only two animals (4%) presented co-infections by three VBA (Bartonella sp., Anaplasma sp. and hemotropic Mycoplasma sp.). The coati is a wild carnivore found in INP, mainly in areas visited by tourists. These animals are frequently seen searching for food in garbage dumps or in tourists’ belongings. The present study expands the host specificity range of B. machadoae, which has been isolated only from rodents until the present moment. Since the zoonotic potential and transmission routes of the detected VBA are not yet known, surveillance in this area is much needed.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Perles

Moraes

Silva

et al. 2023

Sci Rep

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“…Ehrlichia genovariant #6 from the nine-banded armadillo ( Dasypus novemcinctus ) and tayra ( Eira barbara ) are distinct, yet they exhibit high pairwise identity to an Ehrlichia genovariant infecting tayra in Brazil (Supplementary Table 1 ) and to a novel putative species, Ca . Ehrlichia dumleri, recently described from ring-tailed coatis ( Nasua nasua ) in Brazil 34 (99.70% pairwise nucleotide identity; GenBank OM530515). Additional gltA gene typing of Ehrlichia genovariant #6 confirms its genetic similarity to the Ehrlichia genovariant infecting tayra in Brazil (99.65% nucleotide pairwise identity; GenBank OM055650) and Ca .…”

Section: Resultsmentioning

confidence: 99%

Detection of Anaplasma and Ehrlichia bacteria in humans, wildlife, and ticks in the Amazon rainforest

Buysse,

Koual,

Binetruy

et al. 2024

Nat Commun

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Tick-borne bacteria of the genera Ehrlichia and Anaplasma cause several emerging human infectious diseases worldwide. In this study, we conduct an extensive survey for Ehrlichia and Anaplasma infections in the rainforests of the Amazon biome of French Guiana. Through molecular genetics and metagenomics reconstruction, we observe a high indigenous biodiversity of infections circulating among humans, wildlife, and ticks inhabiting these ecosystems. Molecular typing identifies these infections as highly endemic, with a majority of new strains and putative species specific to French Guiana. They are detected in unusual rainforest wild animals, suggesting they have distinctive sylvatic transmission cycles. They also present potential health hazards, as revealed by the detection of Candidatus Anaplasma sparouinense in human red blood cells and that of a new close relative of the human pathogen Ehrlichia ewingii, Candidatus Ehrlichia cajennense, in the tick species that most frequently bite humans in South America. The genome assembly of three new putative species obtained from human, sloth, and tick metagenomes further reveals the presence of major homologs of Ehrlichia and Anaplasma virulence factors. These observations converge to classify health hazards associated with Ehrlichia and Anaplasma infections in the Amazon biome as distinct from those in the Northern Hemisphere.

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“…Magellanica, detected in a Magellanic penguin (Spheniscus magellanicus) from Chile [24]. In the phylogeny based on the dsb gene, Ehrlichia sequences detected in specimens of A. cyanopus (#389) and B. flaveolus (#418) were grouped with Ehrlichia minasensis, suggesting that Ehrlichia minasensis or a closely related genotype might be transmitted by ticks to a variety of hosts besides cattle, such as sloths [68] and birds.…”

Section: Discussionmentioning

confidence: 99%

Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland

Alabí Córdova,

Fecchio,

Calchi

et al. 2024

Microorganisms

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Despite numerous reports of Anaplasmataceae agents in mammals worldwide, few studies have investigated their occurrence in birds. The present study aimed to investigate the occurrence and molecular identity of Anaplasmataceae agents in birds from the Pantanal wetland, Brazil. Blood samples were collected from 93 different species. After DNA extraction, samples positive for the avian β-actin gene were subjected to both a multiplex quantitative real-time (q)PCR for Anaplasma and Ehrlichia targeting the groEL gene and to a conventional PCR for Anaplasmataceae agents targeting the 16S rRNA gene. As a result, 37 (7.4%) birds were positive for Anaplasma spp. and 4 (0.8%) for Ehrlichia spp. in the qPCR assay; additionally, 13 (2.6%) were positive for Anaplasmataceae agents in the PCR targeting the 16S rRNA gene. The Ehrlichia 16S rRNA sequences detected in Arundinicola leucocephala, Ramphocelus carbo, and Elaenia albiceps were positioned closely to Ehrlichia sp. Magellanica. Ehrlichia dsb sequences detected in Agelasticus cyanopus and Basileuterus flaveolus grouped with Ehrlichia minasensis. The 16S rRNA genotypes detected in Crax fasciolata, Pitangus sulphuratus and Furnarius leucopus grouped with Candidatus Allocryptoplasma. The 23S-5S genotypes detected in C. fasciolata, Basileuterus flaveolus, and Saltator coerulescens were related to Anaplasma phagocytophilum. In conclusion, novel genotypes of Anaplasma, Ehrlichia, and Candidatus Allocryptoplasma were detected in birds from the Pantanal wetland.

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Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, ‘Candidatus Ehrlichia dumleri’ and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil

Cited by 8 publications

References 56 publications

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

Detection of Anaplasma and Ehrlichia bacteria in humans, wildlife, and ticks in the Amazon rainforest

Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland

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