Novel amphiphilic compounds effectively inactivate the vaccinia virus

Abstract

a b s t r a c tRecent studies demonstrated the ability of artificial ribonucleases (aRNases, small organic RNA cleaving compounds) to inactivate RNA-viruses via the synergetic effect of viral RNA cleavage and disruption of viral envelope [1,2]. Herein, we describe the antiviral activity of aRNases against DNA-containing vaccinia virus: screening of aRNases of various structures revealed that amphiphilic compounds built of positively charged 1,4-diazabicyclo[2.2.2] octane substituted at the bridge nitrogen atoms with aliphatic residues efficiently inactivate this virus. The first stage was the destruction of viral membrane and structure of surface proteins (electron microscopy data). Thus, 1,4-diazabicyclo[2.2.2] octane-based aRNases are novel universal agents inactivating both RNA-and DNAcontaining viruses.

Self-assembling systems based on diquaternized derivatives of 1,4-diazabicyclo[2.2.2]octane

“…Diquaternized derivatives of DABCO are widely used as ionic liquids, antimicrobial agents, catalysts, etc. [17][18][19][20][21][22][23][24]. In these works, a marked dependence of the functional activity of the DABCO based surfactants on their structure, especially, the hydrophobicity was demonstrated.…”

Glutaraldehyde inactivation of enveloped DNA viruses in the preparation of haemoglobin-based oxygen carriers

“…Thus, after immediate mixing of the virus with GA, 5 mM glycine was added to the suspension, which was then shaken several times. The treated virus samples were then added sequentially to 96well plates (100 lL/well; eight replicates for each sample) [23], in which approximately 100 lL of PK-15 cell suspension had been added to each well. The plates were then incubated for approximately 5-7 days at 37 C under a 5% CO 2 environment.…”

Ribonucleases as antiviral agents

“…Mimetics of RNases of another class have been created based on the conjugates of diazabi cyclo [2.2.2] octane with imidazole; the rate of RNA hydrolysis by these mimetics increases proportionally to the number of positive charges in the molecule [53]. Recently, synthetic RNases were found to act not only on RNA containing viruses through hydrolysis of viral RNA [54], but also on DNA containing viruses, in particular cowpox virus, through the destruction of the virus envelope [55].…”