Ribonucleases as antiviral agents

Ilinskaya, О. N.; Mahmud, Rashed

doi:10.1134/s0026893314040050

Cited by 51 publications

(37 citation statements)

References 54 publications

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“…Binase is a promising therapeutic agent with anticancer [32–36] and antivirus effects [37, 38]. Secretion of RNase by bacilli in the intestinal flora is a natural defense mechanism that counters oncogenesis and viral infections.…”

Section: Discussionmentioning

confidence: 99%

The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

Sokurenko

Ulyanova

Zelenikhin

et al. 2016

Bioinorganic Chemistry and Applications

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Extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. Several species of the Bacillus genus, like Bacillus pumilus, a common producer of extracellular RNase binase, can populate the intestinal microbiome as a colonizing organism. Without involving metal ions as cofactors, binase depolymerizes RNA by cleaving the 3′,5′-phosphodiester bond and generates 2′,3′-cyclic guanosine phosphates in the first stage of a catalytic reaction. Maintained in the reaction mixture for more than one hour, such messengers can affect the human intestinal microflora and the human body. In the present study, we found that the rate of 2′,3′-cGMP was growing in the presence of transition metals that stabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2′,3′-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.

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Section: Discussionmentioning

confidence: 99%

The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

Sokurenko

Ulyanova

Zelenikhin

et al. 2016

Bioinorganic Chemistry and Applications

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“…This in combination with the rich abundance and high activity of ribonucleases, which actively degrade RNA (Ilinskaya and Mahmud 2014), make RNA less stable than DNA. Hence, specific care has to be taken when working with RNA to avoid degradation, both in the laboratory and in the field.…”

Section: Preservation Methodsmentioning

confidence: 99%

Avian transcriptomics: opportunities and challenges

Jax

Kraus

2018

J Ornithol

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Recent developments in next-generation sequencing technologies have greatly facilitated the study of whole transcriptomes in model and non-model species. Studying the transcriptome and how it changes across a variety of biological conditions has had major implications for our understanding of how the genome is regulated in different contexts, and how to interpret adaptations and the phenotype of an organism. The aim of this review is to highlight the potential of these new technologies for the study of avian transcriptomics, and to summarise how transcriptomics has been applied in ornithology. A total of 81 peer-reviewed scientific articles that used transcriptomics to answer questions within a broad range of study areas in birds are used as examples throughout the review. We further provide a quick guide to highlight the most important points which need to be take into account when planning a transcriptomic study in birds, and discuss how researchers with little background in molecular biology can avoid potential pitfalls. Suggestions for further reading are supplied throughout. We also discuss possible future developments in the technology platforms used for ribonucleic acid sequencing. By summarising how these novel technologies can be used to answer questions that have long been asked by ornithologists, we hope to bridge the gap between traditional ornithology and genomics, and to stimulate more interdisciplinary research.

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“…We proposed earlier that mechanisms of antiviral activity of RNases include both the direct action on nucleic acid and indirect effects, that is, intervention into the RNA interference, immunomodulation, and induction of infected cell apoptosis (for review see [8]). We have demonstrated that binase reduced the titer of pandemic influenza A/Hamburg/4/2009 (H1N1pdm), reovirus serotype 1 (Reo 1-Lang), herpes virus type I (pseudorabies), Middle East respiratory syndrome coronavirus (MERS-CoV), and human corona virus (HCoV-229E) in infected Madin-Darby canine kidney (MDCK) epithelial cells, African green monkey kidney (Vero) cells, Madin-Darby bovine kidney (MDBK) epithelial cells, human fetal lung fibroblast (MRC5) cells, and hepatocellular carcinoma (Huh7) cells, respectively [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Ribonuclease fromBacillusActs as an Antiviral Agent against Negative- and Positive-Sense Single Stranded Human Respiratory RNA Viruses

Mahmud

Müller

Romanova

et al. 2017

BioMed Research International

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Bacillus pumilus ribonuclease (binase) was shown to be a promising antiviral agent in animal models and cell cultures. However, the mode of its antiviral action remains unknown. To assess the binase effect on intracellular viral RNA we have selected single stranded negative- and positive-sense RNA viruses, influenza virus, and rhinovirus, respectively, which annually cause respiratory illnesses and are characterized by high contagious nature, mutation rate, and antigen variability. We have shown that binase exerts an antiviral effect on both viruses at the same concentration, which does not alter the spectrum of A549 cellular proteins and expression of housekeeping genes. The titers of influenza A (H1N1pdm) virus and human rhinovirus serotype 1A were reduced by 40% and 65%, respectively. A preincubation of influenza virus with binase before infection significantly reduced viral titer after single-cycle replication of the virus. Using influenza A virus mini genome system we showed that binase reduced GFP reporter signaling indicating a binase action on the expression of viral mRNA. Binase reduced the level of H1N1pdm viral NP mRNA accumulation in A549 cells by 20%. Since the viral mRNA is a possible target for binase this agent could be potentially applied in the antiviral therapy against both negative- and positive-sense RNA viruses.

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Ribonucleases as antiviral agents

Cited by 51 publications

References 54 publications

The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

Avian transcriptomics: opportunities and challenges

Ribonuclease fromBacillusActs as an Antiviral Agent against Negative- and Positive-Sense Single Stranded Human Respiratory RNA Viruses

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