Novel alternative therapy for spinal tuberculosis during surgery: reconstructing with anti-tuberculosis bioactivity implants

Dong, Junfeng; Zhang, Shengmin; Liu, HaoMing; Li, Xinzhi; Liu, Yonghui; Du, Yingying

doi:10.1517/17425247.2014.872625

Cited by 30 publications

(18 citation statements)

References 41 publications

(40 reference statements)

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“…Autologous bone graft implantation has been considered the main technique to successfully repair bone defects. However, in clinical practice, invasiveness at the donor site and inability to absorb large grafts are problematic (Dong et al, 2014;Ghassemi et al, 2018;Weisz and Errico, 2000).…”

Section: Introductionmentioning

confidence: 99%

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Aragón

Feoli

Irusta

et al. 2019

International Journal of Pharmaceutics

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Bone infection is a devastating condition resulting from implant or orthopaedic surgery. Therapeutic strategies are extremely complicated and may result in serious side effects or disabilities. The development of enhanced 3D scaffolds, able to promote efficient bone regeneration, combined with targeted antibiotic release to prevent bacterial colonization, is a promising tool for the successful repair of bone defects. Herein, polymeric electrospun scaffolds composed of polycaprolactone (PCL) nanofibres decorated with poly(lactic-co-glycolic acid) (PLGA) particles loaded with rifampicin were fabricated to achieve local and sustained drug release for more efficient prevention and treatment of infection. The release profile showed an initial burst of rifampicin in the first six hours, enabling complete elimination of bacteria. Sustained and long-term release was observed until the end of the experiments (28 days), facilitating a prolonged effect on the inhibition of bacterial growth, which is in agreement with the common knowledge concerning the acidic degradation of the microparticles. In addition, bactericidal effects against gram negative (Escherichia coli) and gram positive (Staphylococcus aureus) bacteria were demonstrated at concentrations of released rifampicin up to 58 ppm after 24 h, with greater efficacy against S. aureus (13 ppm vs 58 ppm for E. coli). Cell morphology and cytocompatibility studies highlighted the suitability of the fabricated scaffolds to support cell growth, as well as their promising clinical application for bone regeneration combined with prevention or treatment of bacterial infection.

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Section: Introductionmentioning

confidence: 99%

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Aragón

Feoli

Irusta

et al. 2019

International Journal of Pharmaceutics

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“…PDLLA and nHA are two good drug carriers for osteomyelitis, however, the usage of them in osteoarticular TB remains to be further explored [12]. In this study, PDLLA and nHA were used not only as reconstructing materials but also vehicles for drug delivery.…”

Section: Discussionmentioning

confidence: 99%

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Dong

Zhang

et al. 2014

PLoS ONE

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BackgroundReconstruction materials currently used in clinical for osteoarticular tuberculosis (TB) are unsatisfactory due to a variety of reasons. Rifampicin (RFP) is a well-known and highly effective first-line anti-tuberculosis (anti-TB) drug. Poly-DL-lactide (PDLLA) and nano-hydroxyapatite (nHA) are two promising materials that have been used both for orthopedic reconstruction and as carriers for drug release. In this study we report the development of a novel anti-TB implant for osteoarticular TB reconstruction using a combination of RFP, PDLLA and nHA.MethodsRFP, PDLLA and nHA were used as starting materials to produce a novel anti-TB activity implant by the solvent evaporation method. After manufacture, the implant was characterized and its biodegradation and drug release profile were tested. The in vitro cytotoxicity of the implant was also evaluated in pre-osteoblast MC3T3-E1 cells using multiple methodologies.ResultsA RFP/PDLLA/nHA composite was successfully synthesized using the solvent evaporation method. The composite has a loose and porous structure with evenly distributed pores. The production process was steady and no chemical reaction occurred as proved by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD). Meanwhile, the composite blocks degraded and released drug for at least 12 weeks. Evaluation of in vitro cytotoxicity in MC3T3-E1 cells verified that the synthesized composite blocks did not affect cell growth and proliferation.ConclusionIt is feasible to manufacture a novel bioactive anti-TB RFP/PDLLA/nHA composite by the solvent evaporation method. The composite blocks showed appropriate properties such as degradation, drug release and biosafety to MC3T3-E1 cells. In conclusion, the novel composite blocks may have great potential for clinical applications in repairing bone defects caused by osteoarticular TB.

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“…A poly-DL-lactide (PDLLA) nHA (nano-hydroxyapatite) scaffold can be constructed, which is impregnated with antibiotics to function as a drug delivery system (DDS) in spinal TB. 50 The scaffold was found to possess excellent mechanical properties with no cytotoxic effects, encouraging mesenchymal stem cell growth, as well as functioning as a DDS. In the future, it may be possible to deliver other treatments, such as chemotherapy and radiotherapy, to treat cancer patients who are currently considered terminal.…”

Section: Current Limitations and Futurementioning

confidence: 99%

Three-dimensional printing in surgery: a review of current surgical applications

Malik

Darwood

Shaunak

et al. 2015

Journal of Surgical Research

331

220

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Novel alternative therapy for spinal tuberculosis during surgery: reconstructing with anti-tuberculosis bioactivity implants

Cited by 30 publications

References 41 publications

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Composite scaffold obtained by electro-hydrodynamic technique for infection prevention and treatment in bone repair

Preparation, Characterization, and In Vitro Cytotoxicity Evaluation of a Novel Anti-Tuberculosis Reconstruction Implant

Three-dimensional printing in surgery: a review of current surgical applications

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