Novel Alleles of Phosphorus-Starvation Tolerance 1 Gene (PSTOL1) from Oryza rufipogon Confers High Phosphorus Uptake Efficiency

Abstract

Limited phosphorus availability in the soil is one of the major constraints to the growth and productivity of rice across Asian, African and South American countries, where 50% of the rice is grown under rain-fed systems on poor and problematic soils. With an aim to determine novel alleles for enhanced phosphorus uptake efficiency in wild species germplasm of rice Oryza rufipogon, we investigated phosphorus uptake1 (Pup1) locus with 11 previously reported SSR markers and sequence characterized the phosphorus-starvation tolerance 1 (PSTOL1) gene. In the present study, we screened 182 accessions of O. rufipogon along with Vandana as a positive control with SSR markers. From the analysis, it was inferred that all of the O. rufipogon accessions undertaken in this study had an insertion of 90 kb region, including Pup1-K46, a diagnostic marker for PSTOL1, however, it was absent among O. sativa cv. PR114, PR121, and PR122. The complete PSTOL1 gene was also sequenced in 67 representative accessions of O. rufipogon and Vandana as a positive control. From comparative sequence analysis, 53 mutations (52 SNPs and 1 nonsense mutation) were found in the PSTOL1 coding region, of which 28 were missense mutations and 10 corresponded to changes in the amino acid polarity. These 53 mutations correspond to 17 haplotypes, of these 6 were shared and 11 were scored only once. A major shared haplotype was observed among 44 accessions of O. rufipogon along with Vandana and Kasalath. Out of 17 haplotypes, accessions representing 8 haplotypes were grown under the phosphorus-deficient conditions in hydroponics for 60 days. Significant differences were observed in the root length and weight among all the genotypes when grown under phosphorus deficiency conditions as compared to the phosphorus sufficient conditions. The O. rufipogon accession IRGC 106506 from Laos performed significantly better, with 2.5 times higher root weight and phosphorus content as compared to the positive control Vandana. In terms of phosphorus uptake efficiency, the O. rufipogon accessions IRGC 104639, 104712, and 105569 also showed nearly two times higher phosphorus content than Vandana. Thus, these O. rufipogon accessions could be used as the potential donor for improving phosphorus uptake efficiency of elite rice cultivars.

Genetic enhancement of phosphorus starvation tolerance through marker assisted introgression of OsPSTOL1 gene in rice genotypes harbouring bacterial blight and blast resistance

“…One of the key nutrient sources for crop growth is phosphorus which is vital for root growth and for various key metabolic processes including synthesis of membranes and nucleic acids [ 1 , 2 ]. Low P availability is a major constraint for the production of rice in Asia, Africa, and South America [ 3 ]. Soil P deficiency is normally scattered, identifiable by sick patches across field plots.…”

Molecular Analysis and Phenotypic Performances of BC3F2 Upland Rice Lines Containing Alt and Pup1 Loci

“…Lokus tersebut ialah Phosphorus uptake 1 (Pup1) yang di dalam lokus tersebut terdapat gen Phosphorus-Starvation Tolerance (PSTOL1) (Wissuwa et al 1998;Heuer et al 2009;Gamuyao et al 2012). Analisis berbagai genotipe padi menunjukkan bahwa gen PSTOL juga terdapat pada genotipe lain, seperti padi liar Oryza rufipogon (Neelam et al 2017) dan varietas unggul Indonesia (Tasliah et al 2016). Bahkan, gen PSTOL1 diketahui berkaitan dengan peningkatan toleransi tanaman padi terhadap penyakit blas dan hawar daun bakteri (Chithrameenal et al 2018).…”

Deployment of wild relatives for genetic improvement in rice (Oryza sativa)

“…(2018) identified five novel genes linked to two in‐del markers (12‐7 and 12‐16) on chromosome 12 conferring cold tolerance from O. rufipogon . This species is also a source of genes/QTLs for drought tolerance (Luo et al., 2019; Zhang et al., 2017), heat tolerance (Cao et al., 2020; Lei et al., 2013), high phosphorous uptake efficiency (Deng et al., 2018; Neelam, et al., 2017; Neelam, et al., 2017), and tolerance to acidic soils, iron toxicity and the phosphorous deficiency (Brar & Khush, 2006). Recently Quan et al.…”