Genetic enhancement of phosphorus starvation tolerance through marker assisted introgression of OsPSTOL1 gene in rice genotypes harbouring bacterial blight and blast resistance

Chithrameenal, Kannan; Alagarasan, Ganesh; Raveendran, M.; Robin, S.; Meena, S.; Ramanathan, A.; Ramalingam, Jegadeesan

doi:10.1371/journal.pone.0204144

Cited by 21 publications

(17 citation statements)

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“…Significant phenotypic variation has been observed in rice for different traits related to productivity under low soil P conditions, indicating the possibility of increasing rice production in P poor soils [18][19][20]. Breeders are attempting to develop rice cultivars tolerant to soil P-deficiency using the recently discovered and well characterized major QTL, Pup1 [10,21] and some success has been witnessed in this direction [12,22,23]. However, Pup1 is the only major QTL associated with low soil P tolerance identified so far and there is an imminent need to identify additional, novel, non-Pup1 type QTLs (and the genes underlying them) associated with low P tolerance in rice.…”

Section: Discussionmentioning

confidence: 99%

Novel major QTLs associated with low soil phosphorus tolerance identified from the Indian rice landrace, Wazuhophek

et al. 2021

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With an objective of mapping novel low soil P (Phosphorus) tolerance loci in the non-Pup1 type donor rice line, Wazuhophek, we screened a recombinant inbred line (RIL) mapping population consisting of 330 lines derived from the cross Wazuhophek x Improved Samba Mahsuri (which is highly sensitive to low soil P) in a plot with low soil P for tolerance associated traits. Molecular mapping with SSR markers revealed a total of 16 QTLs (seven major and nine minor QTLs), which are associated with low soil P tolerance related traits. Interestingly, a QTL hotspot, harbouring 10 out of 16 QTLs were identified on the short arm of chromosome 8 (flanked by the makers RM22554 and RM80005). Five major QTLs explaining phenotypic variance to an extent of 15.28%, 17.25%, 21.84%, 20.23%, and 18.50%, associated with the traits, plant height, shoot length, the number of productive tillers, panicle length and yield, respectively, were located in the hotspot. Two major QTLs located on chromosome 1, associated with the traits, total biomass and root to shoot ratio, explaining 15.44% and 15.44% phenotypic variance, respectively were also identified. Complex epistatic interactions were observed among the traits, grain yield per plant, days to 50% flowering, dry shoot weight, and P content of the seed. In-silico analysis of genomic regions flanking the major QTLs revealed the presence of key putative candidate genes, possibly associated with tolerance.

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Section: Discussionmentioning

confidence: 99%

Novel major QTLs associated with low soil phosphorus tolerance identified from the Indian rice landrace, Wazuhophek

et al. 2021

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“…The frequency and probability of getting the desired plant mainly depend on the number of genes being used in the crossing programme, genetic linkage, and the method/nature of the crossing program. The stringent phenotypic selection in combination with genotyping have yielded success in the introgression of R genes and the effective nodulating gene in the target lines as observed in previous studies 31 – 35 . Besides, we have selected the genes that are providing race-nonspecific resistance to both Phytophthora and powdery mildew pathogens.…”

Section: Discussionmentioning

confidence: 82%

“…Combining multiple resistance genes or QTLs for broad-spectrum resistance to various diseases will also guarantees the durability of resistance. For instance, Cithrameenal et al 35 pyramided three BB (xa5 + xa13 + Xa21) resistance genes for broad-spectrum resistance to BB along with OsPSTOL 1 gene in rice.. Hittalmani et al 36 and Castro et al 37 combined genes originating from three different parents for rice blast and stripe rust in barley, respectively. Successful application of maker assisted selection depends on several factors including the type of marker, strength of linkage, inheritance of trait of interest etc.…”

Section: Discussionmentioning

confidence: 99%

Improved host-plant resistance to Phytophthora rot and powdery mildew in soybean (Glycine max (L.) Merr.)

Ramalingam

Alagarasan

Palanisamy

et al. 2020

Sci Rep

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Soybean is an important oilseed cum vegetable crop, susceptible to various biotic stresses which is attributed to recent decline in crop productivity. The emergence of virulent biotypes/strains of different plant pathogens necessitates the development of new crop varieties with enhanced host resistance mechanisms. Pyramiding of multiple disease-resistant genes is one of the strategies employed to develop durable disease-resistant cultivars to the prevailing and emerging biotypes of pathogens. The present study, reports the successful introgression of two major R-genes, including Rps2 (Phytophthora rot resistance), Rmd-c (complete-powdery mildew resistance) and effective nodulating gene (rj2) through functional Marker-Assisted Backcross Breeding (MABB) in the genetic background of well-adapted and high yielding soybean varieties, CO 3 and JS 335. We have identified several promising introgressed lines with enhanced resistance to Phytophthora rot and powdery mildew. The improved soybean lines have exhibited medium to high level of resistance against powdery mildew and Phytophthora rot as well as displayed effective nodulation capacity. Our study has proven the generation of resistant genotypes to realize the potential of MABB for achieving host plant resistance in soybean. the improved lines developed can greatly assist the soybean breeding programs in India and other soybean growing countries for evolving disease-resistant varieties. Phytophthora root and stem rot and powdery mildew are the widespread diseases of soybean crop affecting all major plant parts including seeds, stems, leaves, and roots. Many of the high yielding soybean mega-varieties are highly susceptible to both diseases 1-3. Infections by these pathogens cause severe tissue collapse in host plant that negatively impacts plant growth and productivity. Many of the chemical, cultural and biological methods suggested are practically inadequate to combat these diseases successfully 1,4,5. However, a sustainable approach for combating pathogenesis is to introgress the disease resistant (R-genes) and QTL from the identified soybean lines in to cultivars 5. Although many research groups have successfully introgressed R-genes into an elite variety 6,7 through marker-assisted breeding, there are very few reports of utilizing marker-assisted backcross breeding (MABB) method. Successful MABB was reported in soybean for mobilizing the null allele of β-conglycinin α-subunit 8 and Kunitz trypsin inhibitor 9. Following this example, it will be valuable to introgress the R-gene(s) through MABB to achieve high recurrent parent genome recovery (RPG) while eliminating the linkage-drag effect on target chromosome(s).

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“…Pup1 or phosphorus uptake 1 locus is located in chromosome 12, which encodes major Quantitative Trait Locus (QTL) for phosphorus (P) tolerance (Heuer et al, 2009). Chithrameenal et al (2018) stated that majority of the modern rice cultivars lacked Pup1 loci, yet highly susceptibility to P starvation. Therefore, genetic introgression of both P tolerant gene and blast resistance gene into new cultivars is a strategy to yield a higher rice productivity in the future.…”

Section: Discussionmentioning

confidence: 99%

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Hannum

Wahyuningsih

Sinaga

et al. 2020

OnLine Journal of Biological Sciences

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Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease based on the practices by the local farmers. Molecular screening of blast resistance (R) genes was initiated to the 13 accessions using the Polymerase Chain Reaction (PCR) targeting five blast resistance (R) genes, Pup1, Pi-37, Pi-d2, Pi-ta 2 , Pib and Pi-kh. Out of the 13 accessions, the Pi-d2, Pi-ta 2 and Pib were detected in all cultivars, showing 100% of R gene frequency. The second dominant, Pup1 and Pi-37 gene frequencies were 76.9 and 61.5%, respectively while the least dominant, Pi-kh gene frequency was 15.4%. The number of R genes detected among cultivars was ranged between 3 to 6 genes. The genetic associations among thirteen germplasms were determined using unweighted pair group method with arithmetic mean (UPGMA) analysis. Cluster analysis revealed that the least blast-resistant cultivars: Putri Kembar, Silatian and Siganteng, were grouped into cluster 1, showing polymorphism for Pi-d2, Pi-ta 2 and Pi-b. The high-resistant cultivars, Martabe-Sicondong and Sayuti, Sitolas, Pulo Raja, Pulo Pandan, Siporang and Pulo Manggis, were grouped into cluster 2 and 3, respectively. The medium blast-resistant cultivars, IR 64 and Sitampan were grouped into cluster 4, showing polymorphism for Pup1, Pi-d2, Pi-ta 2 and Pi-b. These results indicated that the utilization of blast-resistant cultivars in North Tapanuli was supported by the presence of R genes.

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Genetic enhancement of phosphorus starvation tolerance through marker assisted introgression of OsPSTOL1 gene in rice genotypes harbouring bacterial blight and blast resistance

Cited by 21 publications

References 56 publications

Novel major QTLs associated with low soil phosphorus tolerance identified from the Indian rice landrace, Wazuhophek

Novel major QTLs associated with low soil phosphorus tolerance identified from the Indian rice landrace, Wazuhophek

Improved host-plant resistance to Phytophthora rot and powdery mildew in soybean (Glycine max (L.) Merr.)

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

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