Novel alk-1-enyl ether lipids isolated from Clostridium acetobutylicum

Oulevey, J.; Bahl, Hubert; Thiele, O. W.

doi:10.1007/bf00414729

Cited by 23 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular the wide diversity of glycolipids documented here is not present in those strains assigned to Clostridium cluster I. In addition, the presence of phosphatidylethanolamine in all the strains of Clostridium cluster I examined in previous studies (Goldfine & Johnston, 2005;Guan et al, 2011Guan et al, , 2012Guan et al, , 2013Johnston & Goldfine, 1983;Johnston et al, 2004Johnston et al, , 2010Oulevey et al, 1986;Thiele et al, 1985;Tian et al, 2013) clearly distinguishes them from the strains examined here that do not synthesize phosphatidylethanolamine, which was supported by the absence of phospholipids with free amino groups in the polar lipid analyses. Care should, however, be taken with regard to the conclusions drawn in some of these publications because links are made to some strains that in the past were incorrectly identified.…”

supporting

confidence: 66%

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

Gerritsen

Fuentes

Grievink

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

275

191

View full text Add to dashboard Cite

A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C12–C19 fatty acids, with C16 : 0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1σ lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium , including Clostridium lituseburense (97.2 %), Clostridium glycolicum (96.2 %), Clostridium mayombei (96.2 %), Clostridium bartlettii (96.0 %) and Clostridium irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (<85 %) to the type strain of Clostridium butyricum , the type species of the genus Clostridium . DNA–DNA hybridization with closely related reference strains indicated reassociation values below 32 %. On the basis of phenotypic and genetic studies, a novel genus, Romboutsia gen. nov., is proposed. The novel isolate CRIBT ( = DSM 25109T = NIZO 4048T) is proposed as the type strain of the type species, Romboutsia ilealis gen. nov., sp. nov., of the proposed novel genus. It is proposed that C. lituseburense is transferred to this genus as Romboutsia lituseburensis comb. nov. Furthermore, the reclassification into novel genera is proposed for C. bartlettii , as Intestinibacter bartlettii gen. nov., comb. nov. (type species of the genus), C. glycolicum , as Terrisporobacter glycolicus gen. nov., comb. nov. (type species of the genus), C. mayombei , as Terrisporobacter mayombei gen. nov., comb. nov., and C. irregulare , as Asaccharospora irregularis gen. nov., comb. nov. (type species of the genus), on the basis of additional data collected in this study. In addition, an emendation of the species Peptostreptococcus anaerobius and the order Eubacteriales is provided.

show abstract

supporting

confidence: 66%

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

Gerritsen

Fuentes

Grievink

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

275

191

View full text Add to dashboard Cite

show abstract

“…Recent studies of clostridia have demonstrated that these are major components of the lipidome in C. difficile [3], Clostridiumtetani [8], Clostridium sporogenes , some strains of C. botulinum Group I that are related to C. sporogenes [6], Clostridium psychrophilum [4], and Clostridium acetobutylicum [5,29,30]. We note that three of the glycolipids common to Romboutsia , MHDAG, DHDAG and TriHDAG, were also found in C. difficile .…”

Section: Discussionmentioning

confidence: 99%

The cellular lipids of Romboutsia

Guan

Chen

Gerritsen

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

View full text Add to dashboard Cite

We have examined the lipids of three isolates, Romboutsia lituseburensis, Romboutsia ilealis, and Romboutsia sp. strain FRIFI, of the newly described genus Romboutsia by two-dimensional thin-layer chromatography (2D-TLC) and by liquid chromatography/mass spectrometry (LC/MS). We have found three phospholipids, phosphatidylglycerol (PG), cardiolipin and phosphatidic acid in all three species. A fourth phospholipid, lysyl-PG, was found in R. lituseburensis and strain FRIFI. Polyprenyl-phosphates were identified in the lipid extracts of all three species. Three glycolipids, mono-, di- and tri-hexosyldiacylglycerol, were common to all three species. An additional glycolipid, tetrahexosyl-diacylglycerol was identified in strain FRIFI. Acylated trihexosyldiacylglycerol and acyl-tetrahexosydiacylglycerol were also found in R. ilealis and strain FRIFI. Remarkably, no alk-1-enyl ether lipids (plasmalogens) were present in Romboutsia as distinct from bacteria of the related genus Clostridium in which these ether lipids are common. We have compared the lipidome of Romboutsia with that recently described for Clostridium difficile, which has plasmalogens, no lysyl-PG, and no tetrahexosyl-diacylglycerol. According to 16S rRNA gene sequencing, Romboutsia spp. and Cosmarium difficile are closely related (>95% sequence identity).

show abstract

“…Glycosyl diradyl glycerols containing glucose and galactose in either monoglycosyl or diglycosyl forms were previously identifi ed in C. acetobutylicum ( 17 ) and C. innocuum ( 18,19 ). An unusual feature of the polar lipids of C. tetani is the presence of N-acetyl glucosaminyl diradylglycerol.…”

Section: Discussionmentioning

confidence: 99%

A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani

Johnston

Aygun‐Sunar

Guan

et al. 2010

Journal of Lipid Research

View full text Add to dashboard Cite

Novel alk-1-enyl ether lipids isolated from Clostridium acetobutylicum

Cited by 23 publications

References 7 publications

The cellular lipids of Romboutsia

A phosphoethanolamine-modified glycosyl diradylglycerol in the polar lipids of Clostridium tetani

Contact Info

Product

Resources

About