Novel Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Iron-Saturated Bovine Lactoferrin Nanocarriers for Oral Delivery in Colon Cancer Therapy

Kanwar, Jagat R.; Mahidhara, Ganesh; Kanwar, Rupinder K.

doi:10.2217/nnm.12.29

Cited by 98 publications

(104 citation statements)

References 105 publications

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“…The same dose was then used in our recently published study with a nanoformulated Fe-bLf diet 13 with similar findings. But, in present study instead of Fe-Lf, native form of bLf has been used along with its nanoformulation.…”

Section: In Vivo Studymentioning

confidence: 72%

“…An equal quantity of this diet was given to mice each time to normalize the dosage in metabolic cages. The detailed structure and release profile of NCs has been studied previously 13 and after the success stories of these NCs they have been now targeted against intracellular parasite T. gondii.…”

Section: In Vivo Studymentioning

confidence: 99%

“…13,14 In brief, after euthanizing mice, blood was collected via cardiac puncture and transferred into unheparinized tubes. The blood was allowed to clot by incubating the tubes at RT for 20 minutes.…”

Section: Cytokine Profilementioning

confidence: 99%

“…Therefore, bioavailability of Lf protein at the site of infection does not alter and has shown maximum efficacy. [13][14][15] Earlier successful studies have shown efficacy of these NCs against various tumors 16 and osteoarthritis, 17 and have shown promising results against diseased mice models. Not only Lf protein has been incorporated in these NCs, but also herbal products have been tried against arthritis like diseases, 18 which has divulged multiple availability of these NCs for any drug/molecule/peptide to be loaded.…”

Section: Introductionmentioning

confidence: 99%

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Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Anand¹,

Sehgal²,

Kanwar³

et al. 2015

IJN

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Toxoplasma gondii is a deadly intracellular parasite known to reside in every nucleated cell and known to cause severe complications in immunocompromised host. Standard drugs are cost effective and cause side effects, therefore, there is a necessity for a new drug molecule with immunomodulatory potential. Lactoferrin (Lf) is a natural milk protein, which has shown antimicrobial properties in its nanoformulation using alginate chitosan calcium phosphate bovine lactoferrin nanocapsules (AEC-CCo-CP-bLf-NCs). The present study was aimed to analyze and compare the effect of bovine Lf (bLf) in its native as well as nanoformulation (AEC-CCo-CP-bLf-NC) against coccidian parasite T. gondii. In vitro analysis has shown a significant increase in nitric oxide production and low parasitemia in in vitro cell culture model. In vivo BALB/c mice model have been used to develop human toxoplasmosis model. After treatment with NCs it has substantially increased the bioavailability of the protein and showed comparatively increased levels of reactive oxygen species, nitric oxide production, and Th1 cytokine which helped in parasite clearance. The mechanism of action of NCs has been clarified by immunoreactivity analysis, which showed accumulation of Lf in macrophages of various visceral organs, which is the site of parasite multiplication. Effect of NCs has significantly decreased ( P <0.05) the parasite load in various organs and helped survival of mice till day 25 postinfection. Fe metabolism inside the mice has been found to be maintained even after administration of mono form of Lf, this indicates novelty of Lf protein. From the present study we concluded that nanoformulation did not reduce the therapeutic potential of Lf protein; however, nanoformulation has enhanced the stability of the protein and shown anti-toxoplasmal activity. Our study presents for the first time nanoformulation of Lf protein against Toxoplasma , which has advantages over the standard drug therapy without any side effects.

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Section: In Vivo Studymentioning

confidence: 72%

Section: In Vivo Studymentioning

confidence: 99%

Section: Cytokine Profilementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Anand¹,

Sehgal²,

Kanwar³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…[44][45][46][47][48][49][50]. Another targeting systems for cancer treatment was also investigated with microRNAdirected oncology approach using MRX34 (a liposome-containing miRNA carrier), for usefulness in metastatic liver cancer [51,52].…”

Section: Introductionmentioning

confidence: 99%

Scope of Nanotechnology in Drug Delivery

Maroof¹,

Zafar²,

Ali³

et al. 2015

J Bioequiv Availab

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Advances in Anticancer Protein Delivery using Micro‐/Nanoparticles

Sun

Lü

2014

Part & Part Syst Charact

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Proteins exhibiting anticancer activities, especially those capable of discriminately killing cancer cells, have attracted increasing interest in developing protein-based anticancer therapeutics. This progress report surveys recent advances in delivering anticancer proteins directly to tumor tissue for inducing apoptosis/necrosis or indirectly to antigen presenting cells for provoking immune responses. Protein delivery carriers such as inorganic particles, lipid particles, polymeric particles, DNA/protein based biomacromolecular particles as well as cell based carriers are reviewed with comments on their advantages and limitations. Future challenges and opportunities are also discussed.

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Novel Alginate-Enclosed Chitosan–Calcium Phosphate-Loaded Iron-Saturated Bovine Lactoferrin Nanocarriers for Oral Delivery in Colon Cancer Therapy

Abstract: With the promising results of our study, the future potential of NC-loaded Fe-bLf in chemoprevention and in the treatment of human colon cancer, deserves further investigation for translational research and preclinical studies of other malignancies.

Cited by 98 publications

References 105 publications

Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Scope of Nanotechnology in Drug Delivery

Advances in Anticancer Protein Delivery using Micro‐/Nanoparticles

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