Novel advances in the design of three-dimensional bio-scaffolds to control cell fate: translation from 2D to 3D

Santos, Edorta; Hernández, Rosa Marı́a; Pedraz, José Luís; Orive, Gorka

doi:10.1016/j.tibtech.2012.03.005

Cited by 123 publications

(116 citation statements)

References 89 publications

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“…Moreover, they can play an important role in the continuous production of antibiotics and other therapeutic molecules, the development of synthetic organs, bone regeneration, or the treatment of neurodegenerative diseases [13][14][15].…”

Section: Resultsmentioning

confidence: 99%

Massive, Generic, and Controlled Microencapsulation by Flow Focusing: Some Physicochemical Aspects and New Applications

et al. 2015

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Massive, nearly monodisperse, true microencapsulation of a wide variety of active ingredients within biocompatible shells can be achieved using flow focusing at moderate-high Reynolds numbers, a paradigmatic tool for highly controlled flow chemistry processes whose flexibility and physical aspects are briefly illustrated here. We show that the natural, regular capillary breakup of a laminar high-speed microjet produced by gentle mechanical means alone allows the production of true microcapsules with controlled dimensions. The process versatility is shown in a variety of examples including encapsulation of different materials as proteins and/or microorganisms in biocompatible polymers as poly-L-glutamic acid (PLGA). Microcapsules produced show nearly homogeneous size, well-centered core, and their size and structure are well predicted by simple theoretical models.

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Section: Resultsmentioning

confidence: 99%

Massive, Generic, and Controlled Microencapsulation by Flow Focusing: Some Physicochemical Aspects and New Applications

et al. 2015

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“…There has been a push in recent years to move away from 2D culture systems, and this has been prompted by strong arguments in terms of biological relevance 51,59 . It is worth appreciating at 21 this point that the distinction between 2D and 3D materials becomes immaterial as the molecular scale decreases.…”

Section: The Surface; Between the Bulk Materials And Medium (E)mentioning

confidence: 99%

“…Depending on solvent composition and surface properties, other entities such as counter-ions may also be present. In the biological context, most surfaces adsorb a wide variety of biomolecules such as proteins from a media.There has been a push in recent years to move away from 2D culture systems, and this has been prompted by strong arguments in terms of biological relevance 51,59 . It is worth appreciating at 21 this point that the distinction between 2D and 3D materials becomes immaterial as the molecular scale decreases.…”

mentioning

confidence: 99%

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Hickman

Boocock

Pockley

et al. 2016

ACS Biomater. Sci. Eng.

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Cell and tissue culture has evolved from the use of simple glassware for the propagation of cells and tissues into a comprehensive platform for interrogating complex biological systems, directing cell fate and deriving products with clinical and therapeutic value.However, despite significant advances, current in vitro culture approaches remain limited in their capacity to model the clinical/biological complexities of disease, in part at least due to the deficiencies of existing culture materials. The challenge is therefore to identify innovative materials-based solutions that have greater control over cells in vitro, while better representing biological systems in vivo. Such platforms would be suitable for biomarker discovery and tissue engineering applications. This review examines the development of tissue culture materials, advances in our understanding of cell-surface interactions and the application of this knowledge towards the development of new approaches for better examining biological events.3 he ability to culture cells and tissues in vitro is a fundamental aspect of modern science. Established early in the twentieth century, notably through the work of Harrison R.G. of John Hopkins University 1 , the ability to culture cells and tissues has markedly improved during the intervening period. The field has progressed from an ability to maintain and culture tissue for extended periods, through the discovery and establishment of immortal cell lines, to today, where tissue engineering is making considerable progress in the production of artificial tissues and organs in vitro [2][3][4] . Key to these successes have been advances in the culture surfaces on which cells and tissues are grown.This review summarizes progress in the development of tissue culture materials, highlights current requirements and existing limitations for in vitro culture, and examines their relevance to clinical questions and our current understanding of tissue culture materials design. Although long-established, current culture materials may not always be appropriate for modelling in vivo conditions, and innovative strategies are therefore required in order to overcome existing limitations. Current Issues with Tissue Culture:Numerous articles have highlighted the drawbacks and limitations of current in vitro culture systems 5,6 . Concerns revolve around deficiencies in the culture systems and the tissue they generate. Although the latter can be linked to the quality of the initial cellular material, contamination and/or poor maintenance of historical cell lines 6,7 , it can also result from deficiencies in the culture systems i.e. not all cell populations are amenable to in vitro culture.Problems are compounded once tissue enters in vitro culture, as derived populations are expected to maintain their in vivo relevance. However, cells naturally adapt to the local environment and T 4 prolonged culture of immortalized cell lines results in a progressive divergence from the parental population 6,8,9 . Although loss or gain of ab...

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“…1). [3][4][5] This discrepancy between traditional in vitro culture conditions and the in vivo environment has been recognized among a variety of research areas, including the area of neuroscience and more specifically neurological disease, the latter presenting a great challenge in modern medicine. [6] A glance at our nervous system Our nervous system encompasses two main cell types, neurons and glial cells, which both have a crucial role in nervous system functioning.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

et al. 2017

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To gain a better understanding of the underlying mechanisms of neurological disease, relevant tissue models are imperative. Over the years, this realization has fuelled the development of novel tools and platforms, which aim at capturing in vivo complexity. One example is the field of biofabrication, which focuses on fabrication of three-dimensional (3D) biologically functional products in a controlled and automated manner. Herein, we provide a general overview of classical 3D cell culture platforms, particularly in the context of neurodegenerative disease. Subsequently, the focus is put on bioprinting-based biofabrication, its potential to advance 3D neuronal cell culture and, to conclude, the relevant translational bottlenecks, which will need to be considered as the field evolves.

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Novel advances in the design of three-dimensional bio-scaffolds to control cell fate: translation from 2D to 3D

Cited by 123 publications

References 89 publications

Massive, Generic, and Controlled Microencapsulation by Flow Focusing: Some Physicochemical Aspects and New Applications

Massive, Generic, and Controlled Microencapsulation by Flow Focusing: Some Physicochemical Aspects and New Applications

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

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