Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate

Magnet, Ángela; Henriques‐Gil, Nuno; Galván-Díaz, Ana Luz; Izquiedo, F.; Fenoy, S.; Águila, C. del

doi:10.1007/s00436-014-3945-2

Cited by 45 publications

(16 citation statements)

References 21 publications

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“…K2P has also been applied to study taxonomic divergence in different taxa such as bacteria and protozoa [74,75]. The phylogenetic analysis allowed us to propose that the TS2 isolate belongs to the Cadophora genus, with TS2 grouping directly with other Cadophora species (Fig 1).…”

Section: Resultsmentioning

confidence: 99%

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

et al. 2017

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Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities. Molecular based taxonomic approaches identified these strains as Cadophora sp. TS2, Emericellopsis sp. TS11 and Pseudogymnoascus sp. TS 12. These three fungi displayed psychrotolerance and halotolerant growth on CMC and xylan as sole carbon sources, with optimal growth rates at 20°C. They produced CMCase and xylanase activities, which displayed optimal temperature and pH values of between 50–70°C and pH 5–8 respectively, together with good thermostability and halotolerance. In solid-state fermentations TS2, TS11 and TS12 produced CMCases, xylanases and peroxidase/phenol oxidases when grown on corn stover and wheat straw. This is the first time that CMCase, xylanase and peroxidase/phenol oxidase activities have been reported in these three fungal genera isolated from a marine sponge. Given the biochemical characteristics of these ligninolytic enzymes it is likely that they may prove useful in future biomass conversion strategies involving lignocellulosic materials.

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Section: Resultsmentioning

confidence: 99%

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

et al. 2017

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“…Evolutionary studies of Acanthamoeba have led to the identification of 19 different genotypes (T1-T19) based on rRNA gene sequencing (Lorenzo- Morales et al, 2013;Magnet et al, 2014;Qvarnstrom et al, 2013). Attempts to correlate pathogenicity with certain genotypes continue to be made although it has been shown that 90% of Acanthamoeba isolates that produce infections belong to the T4 genotype, suggesting that the abundance of T4 isolates in human infections is most likely due to their greater virulence and/or other properties that enhance their transmissibility, as well as to their decreased susceptibility to chemotherapeutic agents (Khan, 2006;Lorenzo-Morales et al, 2013, 2015.…”

Section: Introductionmentioning

confidence: 99%

Detection of Acanthamoeba on the ocular surface in a Spanish population using the Schirmer strip test: pathogenic potential, molecular classification and evaluation of the sensitivity to chlorhexidine and voriconazole of the isolated Acanthamoeba strains

Rocha-Cabrera

Reyes-Batllé

Martín-Navarro

et al. 2015

Journal of Medical Microbiology

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“…To date, on the basis of rDNA sequences, the genus has been divided into 20 genotypes (T1–T20) [ 4 ]. To date, several genotypes are described as the causative agent of different diseases, the genotypes T4, T3, and T11 are the most often associated with keratitis [ 5 , 6 ]. Moreover, the species Hartmannella vermiformis, rarely associated with AK [ 7 , 8 ], has been renamed as Vermamoeba vermiformis, because its significant differentiation from all other Hartmannella species [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

Filippo

Santoro

Lovreglio

et al. 2015

IJERPH

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Free-living amoebae (FLA) are protozoa ubiquitous in Nature, isolated from a variety of environments worldwide. In addition to their natural distribution, some species have been found to be pathogenic to humans. In the present study a survey was conducted in order to evaluate the presence and to characterize at molecular level the isolates of amoebic organisms collected from different water sources in Italy. A total of 160 water samples were analyzed by culture and microscopic examination. FLA were found in 46 (28.7%) of the investigated water samples. Groundwater, well waters, and ornamental fountain waters were the sources with higher prevalence rates (85.7%, 50.0%, and 45.9%, respectively). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates. Other FLA species, including Vahlkampfia sp. and Naegleria spp., previously reported in Italy, were not recovered. The occurrence of potentially pathogenic free-living amoebae in habitats related to human population, as reported in the present study, supports the relevance of FLA as a potential health threat to humans.

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Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate

Cited by 45 publications

References 21 publications

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

Detection of Acanthamoeba on the ocular surface in a Spanish population using the Schirmer strip test: pathogenic potential, molecular classification and evaluation of the sensitivity to chlorhexidine and voriconazole of the isolated Acanthamoeba strains

Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

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