Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

Filippo, Margherita Montalbano Di; Santoro, Maristella; Lovreglio, Piero; Monno, Rosa; Capolongo, Carmen; Calia, Carla; Fumarola, Luciana; D’Alfonso, Rossella; Berrilli, Federica; Cave, David Di

doi:10.3390/ijerph120403417

Cited by 47 publications

(16 citation statements)

References 30 publications

(36 reference statements)

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“…Vermamoeba vermiformis is one of the most common FLA to have been isolated from different water systems (Rhoads et al 2015;Montalbano Di Filippo et al 2015;Lu et al 2015;Armand et al 2016). It is also frequently recovered from hospital water networks as shown by several authors (Rohr et al 1998;Thomas et al 2006;Ovrutsky et al 2013;Pagnier et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

et al. 2016

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Free living amoebae (FLA) are protists ubiquitously present in the environment. Aspergillus fumigatus is a mould responsible for severe deep-seated infections, and that can be recovered in the same habitats as the FLA. By conducting coculture experiments and fungal incubation with amoebal supernatants, we report herein that Vermamoeba vermiformis, a FLA present in hospital water systems, promotes filamentation and growth of A. fumigatus. This finding is of particular importance to institutions whose water systems might harbor FLA and could potentially be used by immunocompromised patients. Also, the relationships between V. vermiformis and A. fumigatus were compared to those between this fungus and two other phagocytic cells: Acanthamoeba castellanii, another FLA, and macrophage-like THP-1 cells. After 4 h of coincubation, the percentages of the three phagocytic cell types with adhered conidia were similar, even though the types of receptors between FLA and macrophagic cell seemed different. However, the percentage of THP-1 with internalized conidia was considerably lower (40 %) in comparison with the two other cell types (100 %). Thus, this study revealed that interactions between A. fumigatus and these three phagocytic cell types show similarities, even though it is premature to extrapolate these results to interpret relationships between A. fumigatus and macrophages.

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Section: Introductionmentioning

confidence: 99%

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

et al. 2016

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“…Di Filippo et al study confirmed that culture method is not precise enough to detect of free-living Amoebae [ 8 ]. In the study, a total of 160 water samples was analyzed for FLA by PCR method.…”

Section: Discussionmentioning

confidence: 99%

“…Acanthamoeba has been divided into different genotypes (T1-T20) based on rRNA gene [ 8 ]. Among Acanthamoeba genotypes, the most prevalent type is T4 that cause disease in human [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters

et al. 2018

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BackgroundFree-living amoeba (FLA) are wide-spread protozoa that are found in different environmental sources including water, soil, dust, hospital units and ventilation areas. These amoebas can act as opportunistic or non-opportunistic pathogens. Among FLAs, some genera such as Acanthamoeba are important because of their potential pathogenic ability in humans. The purpose of this study is to identify of Acanthamoeba isolated from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.From August to December 2015, 90 samples were collected from pond water of parks of 13 regions of Mashhad City. Physicochemical parameters were measured in situ. After filtering, the samples were cultured on Bacto-agar enriched with Escherichia coli. PCR analysis was conducted on the culture-positive samples, and then the PCR products were sequenced. Statistical analysis was performed by SPSS software and Fisher’s Exact and Mann-Whitney test.ResultsAmong the samples that were examined, 19 samples (21.1%) were positive for Acanthamoeba. The sequencing revealed that Acanthamoeba isolates belonged to T4 genotype of Acanthamoeba. There was no significant relationship between physicochemical parameters and the presence of Acanthamoeba.ConclusionThe prevalence of Acanthamoeba in pond water of parks was relatively high, but there was no significant relationship between physicochemical parameters and the presence of Acanthamoeba.

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“…However, even the use of automated extraction, PCR and DNA sequencing requires time-consuming procedures consisting in the preliminary steps of isolation of the species of interest [ 4 ]. The paper by Montalbano et al [ 34 ] describes a study to evaluate the presence of free-living amoebae (FLA). Identification of FLA species/genotypes, based on the 18S rDNA regions, allowed to identify 18 (39.1%) Acanthamoeba isolates (genotypes T4 and T15) and 21 (45.6%) Vermamoeba vermiformis isolates.…”

Section: Rapid and Efficient Methods To Monitor The Presence Of Emmentioning

confidence: 99%

Scientific Symposium “Small Solution for Big Water-Related Problems: Innovative Microarrays and Small Sensors to Cope with Water Quality and Food Security”

Marcheggiani

Spurio

Cimarelli

et al. 2015

IJERPH

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This issue presents the conclusive results of two European Commission funded Projects, namely Universal Microarrays for the Evaluation of Fresh-water Quality Based on Detection of Pathogens and their Toxins (MicroAQUA) and Rationally Designed Aquatic Receptors (RADAR). These projects focused their activities on the quality of drinking water as an extremely important factor for public health of humans and animals. The MicroAQUA Project aimed at developing a universal microarray chip for the detection of various pathogens (cyanobacteria, bacteria, viruses and parasitic protozoa) and their toxins in waters. In addition, the project included the detection of select species of diatoms, which represent reliable bio-indicators to assess overall water quality. Large numbers of compounds are released into the environment; some of these are toxins such as endocrine disrupting compounds (EDCs) and can affect the endocrine, immune and nervous systems of a wide range of animals causing alterations such as reproductive disorders and cancer. Detection of these contaminants in water systems is important to protect sensitive environmental sites and reduce the risk of toxins entering the food chain. A modular platform for monitoring toxins in water and food production facilities, using biosensors derived from aquatic organisms, was the main goal of RADAR Project.

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Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

Cited by 47 publications

References 30 publications

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

Vermamoeba vermiformis-Aspergillus fumigatus relationships and comparison with other phagocytic cells

Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters

Scientific Symposium “Small Solution for Big Water-Related Problems: Innovative Microarrays and Small Sensors to Cope with Water Quality and Food Security”

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