Novel 20% doxycycline-loaded PLGA nanospheres as adjunctive therapy in chronic periodontitis in type-2 diabetics: randomized clinical, immune and microbiological trial

Lecio, Giovana; Ribeiro, Fernanda Vieira; Pimentel, Suzana Peres; Reis, Aurélio Amorin; Silva, Rafaela Videira Clima da; Nociti-Jr, Francisco Humberto; Moura, Lucas de Almeida; Duek, Eliane; Casati, Márcio Zaffalon; Casarin, Renato Corrêa Viana

doi:10.1007/s00784-019-03005-9

Cited by 32 publications

(33 citation statements)

References 35 publications

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“…In order to determine the impact of CHX containing surfaces on the expression of inflammatory markers, 3×10 4 cells/well were seeded in 24 well culture plates containing or not cpTi discs with or without CHX for 1, 2, 3 and 4 days. After the experimental periods, secreted levels of the interleukin (IL)-4, IL-6, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were determined using the multiplexing technology following a previous protocol [26].…”

Section: Secretome Assaymentioning

confidence: 99%

Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Matos

Almeida

Beline

et al. 2020

Materials Science and Engineering: C

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Our goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups [no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p<0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p<0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p>0.05), whereas cell metabolism (MTT assay) was affected by CHX, with the 35 min of plasma deposition time group displaying the lowest values as compared to bulk cpTi (p<0.05). Moreover, data analysis showed that films, with or without CHX, significantly affected the expression profile of inflammatory cytokines, including IL-4, IL-6, IL-17, IFN-y and TNF-α by NIH-F3T3 cells (p<0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p>0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates.

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Section: Secretome Assaymentioning

confidence: 99%

Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Matos

Almeida

Beline

et al. 2020

Materials Science and Engineering: C

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“…This study was a parallel, double-blind, randomized, placebo-controlled clinical trial and data for 40 individuals was analysed receiving either a placebo of PLGA nanoparticles or doxycycline loaded nanoparticles. Locally applied doxycycline loaded PLGA nanoparticles favoured cytokine modulation and microbial reduction and were able to additionally reduce pockets and bleeding on probing in the patients [133]. This study shows the clinical promise of polymeric drug loaded nanoparticles as dental materials in the treatment of periodontitis.…”

Section: Polymeric Nanoparticles For Drug Delivery and Immunomodulationmentioning

confidence: 65%

Recent Advances in the Development of Antimicrobial and Antifouling Biocompatible Materials for Dental Applications

et al. 2021

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The risk of secondary bacterial infections resulting from dental procedures has driven the design of antimicrobial and antifouling dental materials to curb pathogenic microbial growth, biofilm formation and subsequent oral and dental diseases. Studies have investigated approaches based primarily on contact-killing or release-killing materials. These materials are designed for addition into dental resins, adhesives and fillings or as immobilized coatings on tooth surfaces, titanium implants and dental prosthetics. This review discusses the recent developments in the different classes of biomaterials for antimicrobial and antifouling dental applications: polymeric drug-releasing materials, polymeric and metallic nanoparticles, polymeric biocides and antimicrobial peptides. With modifications to improve cytotoxicity and mechanical properties, contact-killing and anti-adhesion materials show potential for incorporation into dental materials for long-term clinical use as opposed to short-lived antimicrobial release-based coatings. However, extended durations of biocompatibility testing, and adjustment of essential biomaterial features to enhance material longevity in the oral cavity require further investigations to confirm suitability and safety of these materials in the clinical setting. The continuous exposure of dental restorative and regenerative materials to pathogenic microbes necessitates the implementation of antimicrobial and antifouling materials to either replace antibiotics or improve its rational use, especially in the day and age of the ever-increasing problem of antimicrobial resistance.

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“…These factors can be attributed to the effects of DOX, such as inhibition of MMPs (collagenase and gelatinase) and implementation of a positive impact on the bone repair process. In addition, it provides anti-inflammatory effects by suppressing the number of polymorphonuclear leukocytes and blocking the release of prostaglandin E2 (31)(32)(33). Another important factor is the way DOX is used, since authors stated that DOX gel is able to remain in the subgingival site for a minimum period of ten days, and its mechanism of action thus reduced the volume of the gingival crevicular fluid (GCF) and the transforming growth factor beta 1 (TGF-ß1), with improved clinical signs of inflammation, decreased probe depth and clinical gain of insertion (34,35).…”

Section: Discussionmentioning

confidence: 99%

“…A possible explanation for these results is the fact that DOX directly prevents bone resorption, inducing osteoclastic apoptosis and indirectly inhibiting the activating receptor for nuclear binding factor kappa beta (RANKL) in the genesis of osteoclasts. In addition, it benefits the formation of bone tissue by activating osteoblasts (31)(32)(33). This treatment may have inhibited the connection between the RANK receptor and its ligand, preventing its dimerization and causing a decrease in osteoclastogenic activity, as well as inhibiting the formation of multinucleated TRAP-positive cells, which are expressed mainly in osteoclasts (38).…”

Section: Discussionmentioning

confidence: 99%

Influence of Doxycycline and InGaAlP Diode Laser at 660 nm Wavelength in the Treatment of Periodontitis Induced in Rats: In Vivo Study

Silveira

Lima

Cintra

et al. 2021

Photochem & Photobiology

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Novel 20% doxycycline-loaded PLGA nanospheres as adjunctive therapy in chronic periodontitis in type-2 diabetics: randomized clinical, immune and microbiological trial

Cited by 32 publications

References 35 publications

Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Recent Advances in the Development of Antimicrobial and Antifouling Biocompatible Materials for Dental Applications

Influence of Doxycycline and InGaAlP Diode Laser at 660 nm Wavelength in the Treatment of Periodontitis Induced in Rats: In Vivo Study

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