NOTES: Stomatococcus mucilaginosus gen.nov., sp.nov., ep. rev., a Member of the Family Micrococcaceae

NOTEAn unknown, Gram-positive, ovoid-shaped bacterium isolated from the nose of a mouse was subjected to a polyphasic taxonomic analysis. Comparative 16S rRNA gene sequencing demonstrated that the unknown organism was a member of the family Micrococcaceae and possessed a specific phylogenetic association with Rothia dentocariosa and Stomatococcus mucilaginosus. Phenotypically, the bacterium closely resembled R. dentocariosa and S. mucilaginosus but could be distinguished from these species by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified in the genus Rothia, as Rothia nasimurium sp. nov. In addition, it is proposed that S. mucilaginosus be reclassified in the genus Rothia, as Rothia mucilaginosa comb. nov. Keywords : Rothia, Stomatococcus, taxonomy, phylogeny, 16S rRNARothia dentocariosa is found in the oral cavity and pharynx of man where it forms part of the normal microflora (Schaal, 1992). This species is also now recognized as an opportunistic pathogen causing septicaemia and endocarditis, as well as other serious infections (e.g. Schafer et al., 1979 ;Pape et al., 1979 ;Pers et al., 1987 ;Minato & Abiko, 1984 ; Schiff & Kaplan, 1987). The taxonomic affinities of R. dentocariosa have always been controversial. Primarily because of its cellular morphology, the species has historically been associated with Actinomyces and related genera (Schaal, 1992). In recent years it has become apparent, however, that it is only remotely related to these taxa, and is phylogenetically a member of the family Micrococcaceae (Stackebrandt et al., 1997), exhibiting a specific association with Stomatococcus mucilaginosus. Like R. dentocariosa, S. mucilaginosus is a resident of the human oral cavity and pharynx. R. dentocariosa is presently the only recognized species of the genus Rothia. There is, however, a considerable body of evidence indicating some bio- chemical and serological heterogeneity within the species (e.g. Lesher et al., 1974 ; Schofield & Schaal, 1981 ;Fotos et al., 1984 ;Kronvall et al., 1998). In addition, the existence of a second genomovar of R. dentocariosa has recently been described from humans (Kronvall et al., 1998). It is currently not known whether R. dentocariosa or similar organisms occur in other animal species (Schaal, 1992). During the course of a study of unusual Actinobacteria from animals, we have characterized a novel Gram-positive, facultatively anaerobic coccus from the nose of a healthy mouse which phenotypically resembles the genus Rothia. Based on the results of a polyphasic taxonomic study, we propose a new species, Rothia nasimurium, for this bacterium.Strain CCUG 35957 T was isolated from the nose of a healthy mouse. The unidentified organism was cultured on Columbia blood agar base supplemented with 5 % defribrinated horse blood at 37 mC in air plus 5% CO # . The strain was biochemically characterized by using the API rapid ID32 STREP, API CORYNE and...

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“…The description of Rothia mucilaginosa is as given for Stomatococcus mucilaginosa by Bergan & Kocur (1982).…”

Section: Description Of Rothia Nasimurium Sp Novmentioning

confidence: 99%

“…nov. In addition, we propose the reclassification of S. mucilaginosus (Bergan and Kocur 1982) in the genus Rothia, as Rothia mucilaginosa comb. nov.…”

mentioning

confidence: 99%

Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov.

Collins¹,

Hutson²,

Båverud³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

113

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“…mucilaginosa, formerly classified as Stomatococcus mucilaginosus [1], is a gram-positive, coagulase-negative, nonspore-forming coccus considered as a part of the commensal flora of the oral cavity and the upper respiratory tract in humans [2]. There have been an increasing number of infections reported for R. mucilaginosa, especially among immunocompromised patients [3,4].…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence of Rothia mucilaginosa DY-18: A Clinical Isolate with Dense Meshwork-Like Structures from a Persistent Apical Periodontitis Lesion

et al. 2010

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Rothia mucilaginosa is an opportunistic pathogen in the human oral cavity and pharynx. We found that R. mucilaginosa DY-18, a clinical isolate from a persistent apical periodontitis lesion, had biofilm-like structures. Similar structures were also observed on R. mucilaginosa ATCC25296. To further study these structures, we determined the complete genome sequence of DY-18 and found it a 2.26-Mb chromosome. Regarding stress responsive systems known to affect biofilm formation in many bacteria, DY-18 genome possessed only two sigma factor genes. One of these encoded an additional sigma factor whose promoter-binding activity may be regulated in response to environmental stimuli. Additionally, several genes assigned to two-component signal transduction systems were presented in this genome. To the best of our knowledge, this is the first complete genome of R. mucilaginosa species and our data raise the possibility that this organism regulates the biofilm phenotype through these stress responsive systems.

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“…; both genera are members of the family Micrococcaceae (12,13,16). S. mucilaginosus is considered part of the normal flora of the human mouth and the upper respiratory tract (3). Though it appears to be an organism of low virulence, the recent increase in the number of reported cases of S. mucilaginosus bacteremia has established the pathogenic potential of this organism.…”

mentioning

confidence: 99%

“…When heart infusion broth was used as a basal medium, the isolates hydrolyzed esculin and produced acid from glucose, fructose, sucrose, trehalose, and mannose (3,16). Identification as Micrococcus spp.…”

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confidence: 99%

Antimicrobial susceptibilities of Stomatococcus mucilaginosus and of Micrococcus spp

Eiff

Herrmann

Peters

1995

Antimicrob Agents Chemother

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The in vitro susceptibilities of 63 isolates of Stomatococcus mucilaginosus and of 188 isolates of Micrococcus spp. to 18 antimicrobial agents were determined by the agar dilution method. Many ␤-lactams, imipenem, rifampin, and the glycopeptides were shown to be active in vitro against Stomatococcus and Micrococcus isolates, whereas the activities of antibiotics such as some aminoglycosides, erythromycin, and fosfomycin against an important number of these microorganisms are limited.The predominant class of microorganisms causing bacteremia, especially in neutropenic patients, has changed over the past 15 years from aerobic gram-negative bacilli to gram-positive cocci. Although Staphylococcus aureus remains the most commonly isolated agent, the list of gram-positive pathogens now includes a growing number of commensal bacteria: coagulase-negative staphylococci, viridans group streptococci, and, most recently, Stomatococcus mucilaginosus and Micrococcus spp.; both genera are members of the family Micrococcaceae (12,13,16). S. mucilaginosus is considered part of the normal flora of the human mouth and the upper respiratory tract (3). Though it appears to be an organism of low virulence, the recent increase in the number of reported cases of S. mucilaginosus bacteremia has established the pathogenic potential of this organism. The most frequent clinical presentations include septicemia, endocarditis, and catheter-related sepsis (2,8,9,12,13,15). Micrococcus spp. are found as normal inhabitants of human skin and mucous membranes and are usually disregarded as contaminants in clinical isolates (6). Nevertheless, strains identified as Micrococcus spp. have been involved as causative organisms in septicemia, endocarditis, central nervous system infection, peritonitis, and pneumonia (1,10,12,18).Previous studies have examined the susceptibilities either of single isolates or of a strain collection against selected compounds only (5,12,13,17). However, data on susceptibility patterns of a large number of isolates against a panel of antimicrobial agents are so far not available. Therefore, we tested the in vitro activities of a representative number of commonly used antibiotics against 63 isolates of S. mucilaginosus and against 188 isolates of micrococci.Bacterial strains. S. mucilaginosus was isolated from the mucous membrane of the cheek and gingiva; Micrococcus spp. were isolated additionally from the skin. A total of 36 isolates of S. mucilaginosus and 104 isolates of micrococci were obtained from swabs of 50 healthy persons; a total of 27 isolates of S. mucilaginosus and 84 isolates of micrococci were obtained from swabs of 50 neutropenic patients. At the time of specimen isolation, patients did not reveal any clinical signs of infections and had not been treated previously with antibiotics for at least 7 days.Isolates of S. mucilaginosus were identified on the basis of the following characteristics: the presence of gram-positive cocci in clusters; strong adherence of the usually mucoid, transparent, or whitish orga...

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NOTES: Stomatococcus mucilaginosus gen.nov., sp.nov., ep. rev., a Member of the Family Micrococcaceae

Cited by 85 publications

References 4 publications

Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov.

Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov.

Complete Genome Sequence of Rothia mucilaginosa DY-18: A Clinical Isolate with Dense Meshwork-Like Structures from a Persistent Apical Periodontitis Lesion

Antimicrobial susceptibilities of Stomatococcus mucilaginosus and of Micrococcus spp

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