Notes on Sugar Determination

Somogyi, Michael

doi:10.1016/s0021-9258(18)84465-9

Cited by 1,607 publications

(212 citation statements)

References 3 publications

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“…The enzyme-catalyzed reaction was measured with 2.5mg/ml soluble laminarin as a substrate in a 40mM potassium phosphate buffer, pH 7.0, at 30°C, for 15 min to obtain the reaction-time course. The reducing power caused by the enzymatic reaction from the substrate was determined by Somogyi-Nelson a method by which absorbance at 600 nm was measured (Somogyi, 1945). One unit of the enzyme was defined as the ˶ amount of enzyme ˵ , which liberates 1μmol of reducing sugar equivalent to glucose per one min under the standard assay conditions.…”

Section: 3β-13 Glucanase (Laminarinase)mentioning

confidence: 99%

Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)

Gaafar

Baka

Abou‐Dobara

et al. 2021

Egyptian Journal of Phytopathology

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Roselle (Hibiscus sabdariffa L.) is one of the most crucial medicinal plants all over the world. The present study was carried out with the intention of solving some problems related to soil-borne diseases. Fusarium oxysporum is associated with wilt disease of roselle plant. The application of some microbiota i.e., a mixture of Pseudomonas fluorescens (PSR-11), Bacillus subtilis (BSR-8), Pleurotus ostreatus and mycorrhizeen® individually or in combination were investigated for controlling wilt disease, growth, and yield of roselle plant. The study was conducted in sandy textured soil under sprinkler irrigation of Ismailia Agricultural Research Station, Ismailia, Egypt. Under greenhouse conditions, all the tested bioagents reduced wilt incidence and increased the percentage of survived plants. The conglomeration of the bacterial mixture of Pseudomonas fluorescens and Bacillus subtilis, Pleurotus ostreatus and mycorrhizeen® was more effective in controlling wilt disease than using any of them individually. The application of the investigated bioagents showed a significant increase in phenol, photosynthetic pigments, and dehydrogenase contents, as well as root colonization percent. The obtained results showed the ability of B. subtilis and P. fluorescens to produce lytic enzymes such as β-1-3 glucanase, β-1-4 glucanase, chitinase and protease, in addition to their production of siderophore and HCN. The consortium of all microbiota gave the best results for growth and yield parameters of roselle plant.

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Section: 3β-13 Glucanase (Laminarinase)mentioning

confidence: 99%

Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)

Gaafar

Baka

Abou‐Dobara

et al. 2021

Egyptian Journal of Phytopathology

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“…For quanti cation of reducing and total soluble sugars, powdered seeds incubated in 80 % ethanol (70 0 C for 90 min) were centrifuged at 14000 rpm for 10 min followed by supernatant extraction in rotary evaporator. The residuals obtained were dissolved in 0.5 mg/ml de-ionized water and the absorbance was noted at 620 nm and 490 nm, respectively (Nelson 1944;Somogyi 1952;Focks and Benning, 1998). For determination of galactomannan content, the dried residuals of powdered seeds extracted in phosphorus pentoxide (P 2 O 5 ) and ethanol were hydrolyzed in 2 M tri uoroacetic acid (TFA) followed by membrane lteration and de-ionization in C 18 column.…”

Section: Quanti Cation Of Proteins Sugars and Galactomannanmentioning

confidence: 99%

Increased Seed Size Mediated by Cyamopsis Tetragonoloba Big SEEDS LIKE Silencing Is Positively Co-related to Galactomannan Content, Phytochemical Biosynthesis and Anti-diabetic Potential of Guar

Thakur

Prasad

2021

Preprint

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In higher plants, seed size is an important agronomic trait that determines the crop yield and evolutionary fitness. Guar is an economically important legume crop because of the presence of galactomannan in its seed endosperm which is used as a stabilizer and thickener in pharmaceutical, paper, cosmetic and textile industries. The current study demonstrate the role of CtBSL, in regulation of seed size, weight and galactomannan content in two independent BSL12-25-15-31-3 and BSL70-21-25-4-16 T4 transgenic lines of guar, via. pCMKCtBSL mediated post transcriptional CtBSL silencing approach. The upregulation of CtCYCA2;4, CtCYCD3;2, CtCDKE-1, CtCYCT1;5, CtH2A, CtH2B, CtH3, CtH4, CtERF, CtWRKY43, CtGRF5 and CtGIF1 genes in transgenic lines of guar contributed to enhanced cell proliferation and final seed size which was further strongly and positively correlated to phytochemical biosynthesis and their anti-diabetic, anti-AGEs and lipase inhibitory potential against α-glucosidase, α-amylase, AGEs and lipase enzymes, respectively. Therefore, here we conclude the potential role of CtBSL silencing in improving the crop yield and therapeutic treatment of diabetes and obesity by positive regulation of seed size and phytochemical biosynthesis, respectively, in guar.

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“…The 16S rRNA genes were selectively amplified (Somogyi, 1952). PCR reaction conditions were carried out according to Beffa et al (1996), and the PCR product was cloned into the pGEM-T vector system.…”

Section: Dna Isolation and 16s Rrna Gene Sequence Analysismentioning

confidence: 99%

Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Özer

Rakici

İnan

et al. 2020

Journal of Apitherapy and Nature

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Nine lignin-degrading bacteria were isolated from petroleum-contaminated soil and animal manure samples and characterized by 16S rRNA sequence analysis. Three isolates were identified as Enterobacter cancerogenus, two as Enterobacter ludwigii, one as Citrobacter sedlakii, one as Citrobacter farmeri, one as Klebsiella pneumoniae, and one as Citrobacter murliniae. These bacteria used ligno sulphate as the sole carbon source but did not utilize kraft lignin (KL) as the sole source of carbon and energy. For this reason, basic nutrients, such as 1.0% glucose (w/v) and 0.5% peptone (w/v), were used as additional carbon and nitrogen sources to stimulate bacterial growth for KL decolorization. Under these conditions, the isolates L1, L2, L3, L4, PT21, PT22, PT41, G1, and C1 degraded kraft lignin by 37 %, 14 %, 20%, 43%, 48%, 51%, 28%, 60%, and %99, respectively. The decolorization of Remazol Brilliant Blue R (RBBR) by the isolates was analyzed. The isolates were decolorized at 20-90 % of RBBR, respectively.

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Notes on Sugar Determination

Cited by 1,607 publications

References 3 publications

Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)

Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)

Increased Seed Size Mediated by Cyamopsis Tetragonoloba Big SEEDS LIKE Silencing Is Positively Co-related to Galactomannan Content, Phytochemical Biosynthesis and Anti-diabetic Potential of Guar

Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

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