Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Özer, Ayşegül; Rakici, Erva; İnan, Kadriye; Çanakçı, Sabriye; Beldüz, Ali Osman

doi:10.35206/jan.554729

Cited by 3 publications

(2 citation statements)

References 40 publications

(17 reference statements)

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“…Research has already proved the lignindegrading ability of bacteria under the Enterobacter genus such as E. cancerogenus, E. ludwigii etc. 35 yet no studies have been done on E. oryzendophyticus (reclassified as K. oryzendophytica 36 ) and P. chengduensis. Microbes have developed quite a lot of enzymes for mortifying the diverse constituents of lignocellulosic material.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus

Rajan¹,

Sudhakaran²,

Ravindranath³

et al. 2022

J. Pure Appl. Microbiol.

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The mounting disquiets about the usage of precarious chemicals in the textile industry have steered to the development of eco-friendly and biological methods of fiber processing in the ever-escalating horizon of textile fibers. The current study targets the isolation, identification, and screening of lignin-degrading bacteria from termite gut microflora which could be employed in the textile trade, especially in coir industries for developing a biological method for softening coir fibers. Based on the morphology and taxonomic keys, termites used in the study were identified as Odontotermes obesus. The bacteria isolated from the termite gut having lignolytic activity were picked by using the methylene blue dye decolorizing method. The same was confirmed by using tannic acid. The isolates were then identified as Kosakonia oryzendophytica and Pseudomonas chengduensis by 16s rRNA sequencing. The isolates were further checked for their ability to produce extracellular lignolytic enzymes. The enzyme concentration was found to be significantly higher in the medium containing the microbial consortium than in those with the individual cultures. The consortium filtrate has MnP activity of 41.6 U/mL, LiP activity of 114.3 U/mL, and laccase activity of 61.85 U/mL at 72 hours of incubation. It was found that the enzyme activities were increased considerably until 72 hours of incubation but showed an insignificant increase at 96 hours.

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Section: Resultsmentioning

confidence: 99%

Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus

Rajan¹,

Sudhakaran²,

Ravindranath³

et al. 2022

J. Pure Appl. Microbiol.

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“…E. cancerogenus and E. ludwigii were isolated from petroleum-contaminated soil and animal manure samples in Turkey. They showed ligninolytic activities by degradation of Kraft lignin and RBBR decolorization [ 65 ]. Several Enterobacter species were employed in ligninolytic applications and showed successful performance.…”

Section: Resultsmentioning

confidence: 99%

Klebsiella and Enterobacter Isolated from Mangrove Wetland Soils in Thailand and Their Application in Biological Decolorization of Textile Reactive Dyes

Chantarasiri

2020

IJERPH

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Wastewater released from textile and dye-based industries is one of the major concerns for human and aquatic beings. Biological decolorization using ligninolytic bacteria has been considered as an effective and alternative approach for the treatment of dyeing wastewater. This study aimed to assess the isolation, characterization and application of soil bacteria isolated from mangrove wetlands in Thailand. Four active bacteria were genetically identified and designated as Klebsiella pneumoniae strain RY10302, Enterobacter sp. strain RY10402, Enterobacter sp. strain RY11902 and Enterobacter sp. strain RY11903. They were observed for ligninolytic activity and decolorization of nine reactive dyes under experimental conditions. All bacteria exhibited strong decolorization efficiency within 72 h of incubation at 0.01% (w/v) of reactive dyes. The decolorization percentage varied from 20% (C.I. Reactive Red 195 decolorized by K. pneumoniae strain RY10302) to 92% (C.I. Reactive Blue 194 decolorized by Enterobacter sp. strain RY11902) in the case of bacterial monoculture, whereas the decolorization percentage for a mixed culture of four bacteria varied from 58% (C.I. Reactive Blue 19) to 94% (C.I. Reactive Black 1). These findings confer the possibility of using these bacteria for the biological decolorization of dyeing wastewater.

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Looking into the world’s largest elephant population in search of ligninolytic microorganisms for biorefineries: a mini-review

Rammala

Zhou

2022

Biotechnol Biofuels

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Gastrointestinal tracts (GIT) of herbivores are lignin-rich environments with the potential to find ligninolytic microorganisms. The occurrence of the microorganisms in herbivore GIT is a well-documented mutualistic relationship where the former benefits from the provision of nutrients and the latter benefits from the microorganism-assisted digestion of their recalcitrant lignin diets. Elephants are one of the largest herbivores that rely on the microbial anaerobic fermentation of their bulky recalcitrant low-quality forage lignocellulosic diet given their inability to break down major components of plant cells. Tapping the potential of these mutualistic associations in the biggest population of elephants in the whole world found in Botswana is attractive in the valorisation of the bulky recalcitrant lignin waste stream generated from the pulp and paper, biofuel, and agro-industries. Despite the massive potential as a feedstock for industrial fermentations, few microorganisms have been commercialised. This review focuses on the potential of microbiota from the gastrointestinal tract and excreta of the worlds’ largest population of elephants of Botswana as a potential source of extremophilic ligninolytic microorganisms. The review further discusses the recalcitrance of lignin, achievements, limitations, and challenges with its biological depolymerisation. Methods of isolation of microorganisms from elephant dung and their improvement as industrial strains are further highlighted.

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Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Cited by 3 publications

References 40 publications

Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus

Isolation, Screening and Identification of Lignin Degraders from the Gut of Termites Odontotermes obesus

Klebsiella and Enterobacter Isolated from Mangrove Wetland Soils in Thailand and Their Application in Biological Decolorization of Textile Reactive Dyes

Looking into the world’s largest elephant population in search of ligninolytic microorganisms for biorefineries: a mini-review

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