Notes: Ninhydrin Degradation of Hexosamines and Periodic Acid Oxidation of Hexoses

Studies of lipid A fractions from the lipopolysaccharides of Pseudomonas aeruginosa and Pseudomonas alcaligenes

“…The Morgan-Elson reaction, as described by Strominger et al (1959), was applied to N-acetylated glucosamine disaccharides: the reaction was repeated after acidic hydrolysis (6.1 M-HCl at 105°C for 4h) of the compounds and N-acetylation of the glucosamine liberated. Ninhydrin degradation of free glucosamine disaccharide was carried out by the method of Stoffyn (1959). The disaccharide and related compounds were reduced by treatment with 2% (w/v) NaBH4 for 24h at room temperature.…”