NOTES: Campylobacter jejuni subsp. doylei subsp. nov., a Subspecies of Nitrate-Negative Campylobacters Isolated from Human Clinical Specimens

Steele, T W; Owen, Robert J.

doi:10.1099/00207713-38-3-316

Cited by 56 publications

(31 citation statements)

References 12 publications

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“…doylei strains. The inability of many C. jejuni PCR tests to recognize strains of the latter taxon suggests that the genetic difference between the two subspecies (23,30) contributes to PCR failure. Furthermore, genetic heterogeneity in C. jejuni subsp.…”

Section: Discussionmentioning

confidence: 99%

“…Suspect C. jejuni subsp. doylei isolates should be confirmed by examining for their inability to reduce nitrate (30) or by alternative genetic methods such as AFLP fingerprinting (23). We now routinely use the multiplex PCR for concurrent identification and discrimination of C. jejuni and C. coli (38) as a first-line identification method and specific assays for these two species (13) as required.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

On¹,

Jordan²

2003

J Clin Microbiol

104

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We examined the sensitivity and specificity of 11 PCR assays described for the species identification of Campylobacter jejuni and Campylobacter coli by using 111 type, reference, and field strains of C. jejuni, C. coli, and Campylobacter lari. For six assays, an additional 21 type strains representing related Campylobacter, Arcobacter, and Helicobacter species were also included. PCR tests were initially established in the laboratory by optimizing conditions with respect to five type and reference strains of C. jejuni, C. coli, and C. lari. One PCR test for C. coli failed to give appropriate results during this initial setup phase and was not evaluated further. The remaining 10 assays were used to examine heated lysate and purified DNA templates as appropriate of well-characterized type, reference, and field strains of C. jejuni (n ‫؍‬ 62), C. coli (n ‫؍‬ 34), and C. lari (n ‫؍‬ 15). The tests varied considerably in their sensitivity and specificity for their respective target species. No assay was found to be 100% sensitive and/or specific for all C. jejuni strains tested, but four assays for C. coli gave appropriate responses for all strains examined. Between one and six strains of C. jejuni gave amplicons in four of seven C. jejuni PCR tests only where purified DNA was used as the template; corresponding results were seen with one strain of C. coli in each of three assays for the latter species. Our findings indicate that a polyphasic strategy for PCR-based identification should be used to identify C. jejuni and C. coli strains. The data may assist laboratories in selecting assays suited for their needs and in designing evaluations of future PCR tests aimed to identify these species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

On¹,

Jordan²

2003

J Clin Microbiol

104

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“…The name Campylobacter jejuni subsp. doylei was given to the nitrate-negative Campylobacter strains (51). Several of these recently described Campylobacter taxa have characteristics that do not correspond to the characteristics given in the description of the genus Campylobacter in Bergey 's Manual of Systematic Bacteriology (48).…”

Section: Discussionmentioning

confidence: 99%

Revision of Campylobacter, Helicobacter, and Wolinella Taxonomy: Emendation of Generic Descriptions and Proposal of Arcobacter gen. nov.

Vandamme

Falsen

Rossau

et al. 1991

International Journal of Systematic Bacteriology

673

355

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Hybridization experiments were carried out between DNAs from more than 70 strains of Campylobacter spp. and related taxa and either 3H-labeled 23s rRNAs from reference strains belonging to Campylobacter fetus, Campylobacter concisus, Campylobacter sputorum, Campylobacter coli, and Campylobacter nitrofigilis, an unnamed Campylobacter sp. strain, and a Wolinella succinogenes strain or 3H-or 14C-labeled 23s rRNAs from 13 gram-negative reference strains. An immunotyping analysis of 130 antigens versus 34 antisera of campylobacters and related taxa was also performed. We found that all of the named campylobacters and related taxa belong to the same phylogenetic group, which we name rRNA superfamily VI and which is far removed from the gram-negative bacteria allocated to the five rRNA superfamilies sensu De Ley. There is a high degree of heterogeneity within this rRNA superfamily. Organisms belonging to rRNA superfamily VI should be reclassified in several genera. We propose that the emended genus Campylobacter should be limited to Campylo bacter fetus, Campylo bacter hy oin testinalis , Campylo bacter concisus, Campylo bacter m ucosalis , Campylobacter sputorum, Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and "Campylobacter upsaliensis. " Wolinella curva and Wolinella recta are transferred to the genus Campylobacter as Campylobacter curvus comb. nov. and Campylobacter rectus comb. nov., respectively. Bacteroides gracilis and Bacteroides ureolyticus are generically misnamed and are closely related to the genus Campylobacter. Campylobacter nitrofigilis, Campylobacter cryaerophila, and an unnamed Campylobacter sp. strain constitute a new genus, for which the name Arcobacter is proposed; this genus contains two species, Arcobacter nitrofigilis comb. nov. (type species) and Arcobacter cryaerophilus comb. nov. Wolinellu succinogenes so far is the only species of the genus Wolinella. The genus Helicobacter is also emended; Campylobacter cinaedi and Campylobacter fennelliae are included in this genus as Helicobacter cinaedi comb. nov. and Helicobacter fennelliae comb. nov., respectively. The genus "Flexispira," with "Flexispira rappini" as the only species, is closely related to the genus Helicobacter. The free-living, sulfur-reducing campylobacters do not belong to any of these genera; they probably constitute a distinct genus within rRNA superfamily VI.At present, the genus Campylobacter consists of 13 welldefined species (40). Recently, two additional species, Campylobacter pylori and Campylobacter mustelae, were included in the new genus Helicobacter as Helicobacter pylori and Helicobacter mustelae, respectively (20). The clinical significance of all of these organisms was reviewed recently by Penner (40). A study of the taxonomic structure of the genus Campylobacter in which partial 16s rRNA sequence analysis was used revealed that the Campylobacter species can be divided into three major rRNA homology groups (58 It was the aim of this study to include all known campylobacters and possible relatives in...

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“…Mter addition of the test substrates, dispensing, steriziliation and inoculation we carried out incubation under microaerophilic conditions at 42 °C. The isolated strains were identified according to the latest edition of Bergey's Manual (Krieg and Holt 1984) and in the light ofthe published data on newly accepted campylobacters (Benjamin et al 1983;Gebhart et al 1985;McClung et al 1983;Neil et al 1985;Roop et al 1985;Steele and Owen 1988;Tanner et al 1981 andTotten et al 1985). See Table 1.…”

Section: Methodsmentioning

confidence: 99%

Campylobacter jejuni in Slaughtered Chickens from the Viewpoint of Food Hygiene

Maťašovská¹,

Sladka²,

Mráz³

et al. 1992

Acta Vet. Brno

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MatdovsU Norika, Olga SladU, o. Mdz, Z. MatyU and Iva Tomancova: Campylobacter jejuni in Slaughtered Chickens from the Viewpoint of Food Hygiene. Acta vet. Bmo, 61, 1992: 61-67.A total of 440 slaughtered chickens coming from 27 farms were examined for presence of Campylobacter jejuni between 1st Feb. 1990 and 31st Tan. 1991. The relevant specimens were taken from the outer and inner carcass surfaces, ileum contents, liver parenchyma and bile from groups of 10 birds each week. Isolation attempts yielded 366 C. jejuni strains. Of the 38 and 47 (10.3 % and 12.8 %) were isolated from the outer and inner carcass surfaces, respectively, 121 (35 %) from ileum contents, 92 (25 %) from liver parenchyma and 68 (18.6 %) from bile.The proportions of contaminated chicken carcasses showed two peaks, reaching 62.5 % and 62 % in Mayand October, respectively. An almost parallel trend vas recorded for the isolations from ileum contents and liver parenchyma where the highest number of strains (45 % and 30 % respectively) coincided with the May peak, whereas the highest proportions of isolations from bile (30 %) coincided rather with the second peak. The proportions of C. jejuni isolated from the outer and inner carcass surfaces were relatively low, averaging 9.4 % and 11.4 % respectively, and did not show much fluctuation. The proportions of C. jejuni isolated from bile averaged 13.8 %.In 82 (22.4 %) C. jejuni strains solitary colonies were observed also after control aerobic incubation at 42 °c, but attempts at their further passage under these conditions yielded negative results. C. jejuni carriers and shedders as well as the incidence of asymptomatic C. jejuni infection among chickens do not urge either the farmer or the veterinarian to take any measures, but the consumption of C.jejuni-infected chicken meat constitutes a major health hazard for man. Campylobacter jejuni, slaughtered chickens, food hygieneCampylobacter jejuni was first described as Vibrio jejuni a ones et a1. 1931) and is also known under the names of Vibrio hepaticus (Mathey and Rissberger 1964) or Campylobacter fetus subsp. jejuni (Smibert 1974). It is a causative agent of abortion in sheep, diarrhoea in calves and lambs, campylobacter hepatitis in chickens and febrile enteritis in man.A point of particular epidemiological importance is the fact that C. jejuni can also be iso'ated from the intestinal tract of clinically healthy cattle, sheep, goats, pigs, rabbits, domestic fowls, nirkeys, ducks, pigeons, dogs, cats, monkeys, sparrows, blackbirds and starlings (Smibert 1984). From the intestinal tract it can make its way to the liver and, upon evisceration, also to the outer and inner surfaces of farm animal carcasses. In the domestic fowl this possibility has been demonstrated:(1) on the outer sUrface after scalding and plucking (Baker et al. 1987) in 20 % of the carcasses;(2) on the inner surface after evisceration (Pl!gfimkova 1986, Marinescu et al. 1987 Mfl!-kova 1987) in 28.7 % to 92 % of the carcasses; (3) in the liver (Khalafalla 1990) in 40...

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NOTES: Campylobacter jejuni subsp. doylei subsp. nov., a Subspecies of Nitrate-Negative Campylobacters Isolated from Human Clinical Specimens

Cited by 56 publications

References 12 publications

Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

Revision of Campylobacter, Helicobacter, and Wolinella Taxonomy: Emendation of Generic Descriptions and Proposal of Arcobacter gen. nov.

Campylobacter jejuni in Slaughtered Chickens from the Viewpoint of Food Hygiene

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