Note: Comparison of antibody- and genome-based diagnostic techniques forSugarcane mosaic virus in sugarcane

Balamuralikrishnan, M.; Doraisamy, Sabitha; Ganapathy, T.; Viswanathan, R.

doi:10.1007/bf02980859

Cited by 12 publications

(4 citation statements)

References 5 publications

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“…Serological detection is a simple, rapid, and low-cost method for plant viruses, including agar gel immunodiffusion (AGID), electroblot immunoassay (EBIA), double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), direct antigen coating ELISA (DAC-ELISA), indirect ELISA, and dot enzyme-linked immunosorbent assay (Dot-ELISA) (also dot immunobinding assay, DIBA; or dot-bolt immunobinding assays, DBIA) [ 74 , 130 , 131 ]. Hema et al [ 74 ] detected SCSMV by AGID, DAC-ELISA and EBIA.…”

Section: Diagnosis/identificationmentioning

confidence: 99%

Section: Diagnosis/identificationmentioning

confidence: 99%

“…Wang et al [134] established a high-throughput DAC-ELISA method for the detection of SrMV. However, serological detection requires specific antiserums, and its sensitivity is relatively lower than molecular detection techniques [130,131] [83]. These molecular detection techniques have a high sensitivity and specificity and can identify virus species rapidly and accurately.…”

Section: Serological Detectionmentioning

confidence: 99%

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Sugarcane Mosaic Disease: Characteristics, Identification and Control

Wang

et al. 2021

Microorganisms

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Mosaic is one of the most important sugarcane diseases, caused by single or compound infection of Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and/or Sugarcane streak mosaic virus (SCSMV). The compound infection of mosaic has become increasingly serious in the last few years. The disease directly affects the photosynthesis and growth of sugarcane, leading to a significant decrease in cane yield and sucrose content, and thus serious economic losses. This review covers four aspects of sugarcane mosaic disease management: first, the current situation of sugarcane mosaic disease and its epidemic characteristics; second, the pathogenicity and genetic diversity of the three viruses; third, the identification methods of mosaic and its pathogen species; and fourth, the prevention and control measures for sugarcane mosaic disease and potential future research focus. The review is expected to provide scientific literature and guidance for the effective prevention and control of mosaic through resistance breeding in sugarcane.

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Section: Diagnosis/identificationmentioning

confidence: 99%

Section: Diagnosis/identificationmentioning

confidence: 99%

Section: Serological Detectionmentioning

confidence: 99%

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Sugarcane Mosaic Disease: Characteristics, Identification and Control

Wang

et al. 2021

Microorganisms

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“…They also found that this virus moved more slowly in the moderately resistant sugarcane cultivars than in the susceptible ones. Furthermore, by comparing antibody-based tests with genome-based techniques, Balamuralikrishnan et al (2004) determined that the RT-PCR assay method was more sensitive for detecting SCMV in sugarcane plants with less predominant symptoms than ELISA tests.…”

Section: Introductionmentioning

confidence: 99%

Incidence of virus diseases in maize fields in the Trakya region of Turkey

İlbağı

Rabenstein

Habekuß

et al. 2007

phyto

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A survey on maize virus diseases was conducted in the Trakya region of Turkey by examining 32 496 and 46 871 plants in 2004 and 2005, respectively. Rates of symptomatic plants were estimated at 3.7 to 63.6%, depending on locations. Biological and serological test results revealed the presence of barley yellow dwarf virus-PAV (BYDV-PAV), maize dwarf mosaic virus (MDMV), sugarcane mosaic virus (SCMV), and Johnson grass mosaic virus (JGMV). One hundred forty-two samples were collected randomly from 6492 symptomatic plants in 2004. Seventy-two out of the 142 samples were infected with MDMV, two were infected with BYDV-PAV, 19 with MDMV and BYDV-PAV, two with MDMV, BYDV-PAV and SCMV, and only one sample contained the four viruses. In 2005, 100 other leaf samples were collected randomly from 11 739 symptomatic maize plants. Serological tests revealed that 50% of the samples were infected with MDMV and SCMV; however, five showed mixed infections of two or three combinations of tested viruses. Individual MDMV, SCMV, BYDV-PAV and JGMV infections were detected in five, three, two and four samples, respectively. Presence of MDMV was confirmed by Western blot analysis and IC-RT-PCR. SCMV was also detected by IC-RT-PCR. This is the first study reporting the detection of SCMV and JGMV on maize plants in Turkey.Une enquête sur les maladies virales du maïs a été menée dans la région de Trakya en Turquie avec l’examen, en 2004 et 2005, de respectivement 32 496 et 46 871 plantes. Selon l’endroit, le taux de plantes symptomatiques a varié entre 3,7 et 63,6 %. Les résultats de tests biologiques et sérologiques ont révélé la présence de la souche PAV du virus de la jaunisse nanisante de l’orge (BYDV-PAV), du virus de la mosaïque nanisante du maïs (MDMV), du virus de la mosaïque de la canne à sucre (SCMV) et du virus de la mosaïque du sorgho d’Alep (JGMV). En 2004, 142 échantillons foliaires ont été recueillis aléatoirement sur 6492 plantes présentant des symptômes. Soixante-douze des 142 échantillons étaient infectés par le MDMV, deux étaient infectés par le BYDV-PAV, 19 par le MDMV et le BYDV-PAV, deux par le MDMV, le BYDV-PAV et le SCMV, alors qu'un seul contenait les quatre virus. En 2005, 100 nouveaux échantillons foliaires ont été recueillis aléatoirement de 11 739 plants de maïs présentant des symptômes. Des tests sérologiques ont montré que 50 % des échantillons étaient infectés par le MDMV et le SCMV; cependant, cinq échantillons étaient infectés par des combinaisons de deux ou trois des virus testés. Des infections par un seul virus parmi le MDMV, le SCMV, le BYDV-PAV et le JGMV ont été respectivement détectées dans cinq, trois, deux et quatre échantillons. La présence du MDMV a été confirmée par buvardage Western et par immunocapture suivie de transcription inverse et réaction en chaîne de la polymérase (IC-RT-PCR). Le SCMV a aussi été détecté par IC-RT-PCR. La présente étude est la première à signaler, en Turquie, la présence du SCMV et du JGMV dans le maïs

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Diagnosis and Detection of Plant Virus and Viroid Diseases

Sastry

2013

Plant Virus and Viroid Diseases in the Tropics

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Note: Comparison of antibody- and genome-based diagnostic techniques forSugarcane mosaic virus in sugarcane

Cited by 12 publications

References 5 publications

Sugarcane Mosaic Disease: Characteristics, Identification and Control

Sugarcane Mosaic Disease: Characteristics, Identification and Control

Incidence of virus diseases in maize fields in the Trakya region of Turkey

Diagnosis and Detection of Plant Virus and Viroid Diseases

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