Notch regulates the switch from symmetric to asymmetric neural stem cell division in the <i>Drosophila</i> optic lobe

Egger, Boris; Gold, Katrina S.; Brand, Andrea H.

doi:10.1242/dev.051250

Cited by 151 publications

(207 citation statements)

References 46 publications

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“…E(spl)md, E(spl)m7, and Tom are direct targets of the Notch pathway (Bray 2006), while H is a transcription corepressor involved in repression of Notch signaling activity (Bang et al 1995). The Notch pathway is required for neuroepithelial maintenance and expansion (Egger et al 2010;Ngo et al 2010;Reddy et al 2010;Yasugi et al 2010;Orihara-Ono et al 2011;Wang et al 2011b;Weng et al 2012). Our microarray data support a model in which JAK/STAT acts upstream of Notch signaling in the optic lobe (Wang et al 2011a).…”

Section: Discussionsupporting

confidence: 68%

Evidence for Tissue-Specific JAK/STAT Target Genes inDrosophilaOptic Lobe Development

et al. 2013

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The evolutionarily conserved JAK/STAT pathway plays important roles in development and disease processes in humans. Although the signaling process has been well established, we know relatively little about what the relevant target genes are that mediate JAK/STAT activation during development. Here, we have used genome-wide microarrays to identify JAK/STAT targets in the optic lobes of the Drosophila brain and identified 47 genes that are positively regulated by JAK/STAT. About two-thirds of the genes encode proteins that have orthologs in humans. The STAT targets in the optic lobe appear to be different from the targets identified in other tissues, suggesting that JAK/STAT signaling may regulate different target genes in a tissue-specific manner. Functional analysis of Nop56, a cell-autonomous STAT target, revealed an essential role for this gene in the growth and proliferation of neuroepithelial stem cells in the optic lobe and an inhibitory role in lamina neurogenesis.

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Section: Discussionsupporting

confidence: 68%

Evidence for Tissue-Specific JAK/STAT Target Genes inDrosophilaOptic Lobe Development

et al. 2013

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“…Whereas VNC and central brain NBs originate from embryonic NBs, optic lobe NBs arise only during larval stages (Egger et al, 2007). As excellent recent reviews have covered the special mode of neurogenesis in the optic lobes (Egger et al, 2010), we will focus on larval central brain type I and type II NBs.…”

Section: Introductionmentioning

confidence: 99%

Drosophila neuroblasts: a model for stem cell biology

2012

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SummaryDrosophila neuroblasts, the stem cells of the developing fly brain, have emerged as a key model system for neural stem cell biology and have provided key insights into the mechanisms underlying asymmetric cell division and tumor formation. More recently, they have also been used to understand how neural progenitors can generate different neuronal subtypes over time, how their cell cycle entry and exit are coordinated with development, and how proliferation in the brain is spared from the growth restrictions that occur in other organs upon starvation. In this Primer, we describe the biology of Drosophila neuroblasts and highlight the most recent advances made using neuroblasts as a model system.

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“…Notch signaling is required to regulate the progression of the proneural wave (Egger et al, 2010;Ngo et al, 2010;Reddy et al, 2010;Wang et al, 2011;Weng et al, 2012;Yasugi et al, 2010). Given that Cno and Notch functionally interact in other systems (Carmena et al, 2006;Miyamoto et al, 1995) and that cno mutant phenotypes were very reminiscent of those previously described for Notch mutants in the neuroepithelium (Yasugi et al, 2010), we decided to analyze the relative localization of Cno and Notch in the optic lobe.…”

Section: Cno Colocalizes With Notch At the Ajs Of Ne Cellsmentioning

confidence: 99%

“…3A-B0). (Egger et al, 2010;Wang et al, 2011;Weng et al, 2012). First, we wanted to analyze the Notch activity in wild-type brains.…”

Section: Cno Colocalizes With Notch At the Ajs Of Ne Cellsmentioning

confidence: 99%

“…Differentiation of NE cells to medulla NBs progresses from the medial to the lateral edge of the OPC in a 'proneural wave', which was recently identified by the transient and local expression at the wave front of the proneural gene lethal of scute (l9sc) (Yasugi et al, 2008). Over the past few years multiple signaling pathways have proved to be essential for regulating the proneural wave progression within the neuroepithelium in a tissue-autonomous way, including Notch, JAK-STAT, EGFRRas-PointedP1 (PntP1) and Fat-Hippo signaling pathways (Egger et al, 2010;Ngo et al, 2010;Reddy et al, 2010;Yasugi et al, 2010;Yasugi et al, 2008). However, not much is known about non-autonomous regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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A Serrate-Notch-Canoe complex mediates glial-neuroepithelial cell interactions essential during Drosophila optic lobe development

Pérez-Gómez

Slováková

Rives-Quinto

et al. 2013

Journal of Cell Science

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It is firmly established that neuron-glia interactions are fundamental across species for the correct establishment of a functional brain. Here, we found that the glia of the Drosophila larval brain display an essential non-autonomous role during the development of the optic lobe. The optic lobe develops from neuroepithelial cells that proliferate by dividing symmetrically until they switch to asymmetric/differentiative divisions generating neuroblasts. The proneural gene lethal of scute (l'sc) is transiently activated by the Epidermal Growth Factor Receptor (EGFR)/Ras signal transduction pathway at the leading edge of a proneural wave that sweeps from medial to lateral neuroepithelium promoting this switch. This process is tightly regulated by the tissue-autonomous function within the neuroepithelium of multiple signaling pathways, including EGFR/Ras and Notch. This study shows that the Notch ligand Serrate (Ser) is expressed in the glia and it forms a complex in vivo with Notch and Canoe, which colocalize at the adherens junctions of neuroepithelial cells. This complex is crucial for glial-neuroepithelial cell interactions during optic lobe development. Ser is tissue-autonomously required in the glia where it activates Notch to regulate its proliferation, and non-autonomously in the neuroepithelium where Ser induces Notch signaling to avoid the premature activation of the EGFR/Ras pathway and hence of L'sc. Interestingly, different Notch activity reporters showed very different expression patterns in the glia and in the neuroepithelium, suggesting the existence of tissue-specific factors that promote the expression of particular Notch target genes or/and a reporter response dependent on different thresholds of Notch signaling.

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Notch regulates the switch from symmetric to asymmetric neural stem cell division in the Drosophila optic lobe

Cited by 151 publications

References 46 publications

Evidence for Tissue-Specific JAK/STAT Target Genes inDrosophilaOptic Lobe Development

Evidence for Tissue-Specific JAK/STAT Target Genes inDrosophilaOptic Lobe Development

Drosophila neuroblasts: a model for stem cell biology

A Serrate-Notch-Canoe complex mediates glial-neuroepithelial cell interactions essential during Drosophila optic lobe development

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