Evidence for Tissue-Specific JAK/STAT Target Genes in<i>Drosophila</i>Optic Lobe Development

Wang, Hongbin; Chen, Xi; Teng, Hui; Zhou, Yanna; Luo, Hong

doi:10.1534/genetics.113.155945

Cited by 29 publications

(27 citation statements)

References 56 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have described shep as homologous to the vertebrate genes, Rbms2/Scr3 (Armstrong et al 2006) or Rbms1/Scr2/MSSP-2 (Wang et al 2013). Our phylogenetic analysis supported the placement of SHEP in the MSSP family, with the ELAV family of RNA-binding proteins being the next most closely related ( Figure 3B).…”

Section: Identification Of Shep Through Splice-trap Screeningsupporting

confidence: 79%

Neuronal Remodeling During Metamorphosis Is Regulated by the alan shepard (shep) Gene in Drosophila melanogaster

Chen

Bjorum

et al. 2014

Genetics

View full text Add to dashboard Cite

Peptidergic neurons are a group of neuronal cells that synthesize and secrete peptides to regulate a variety of biological processes. To identify genes controlling the development and function of peptidergic neurons, we conducted a screen of 545 splicetrap lines and identified 28 loci that drove expression in peptidergic neurons when crossed to a GFP reporter transgene. Among these lines, an insertion in the alan shepard (shep) gene drove expression specifically in most peptidergic neurons. shep transcripts and SHEP proteins were detected primarily and broadly in the central nervous system (CNS) in embryos, and this expression continued into the adult stage. Loss of shep resulted in late pupal lethality, reduced adult life span, wing expansion defects, uncoordinated adult locomotor activities, rejection of males by virgin females, and reduced neuropil area and reduced levels of multiple presynaptic markers throughout the adult CNS. Examination of the bursicon neurons in shep mutant pharate adults revealed smaller somata and fewer axonal branches and boutons, and all of these cellular phenotypes were fully rescued by expression of the most abundant wild-type shep isoform. In contrast to shep mutant animals at the pharate adult stage, shep mutant larvae displayed normal bursicon neuron morphologies. Similarly, shep mutant adults were uncoordinated and weak, while shep mutant larvae displayed largely, although not entirely, normal locomotor behavior. Thus, shep played an important role in the metamorphic development of many neurons. P EPTIDERGIC neurons produce small peptides, called neuropeptides, which are secreted within the nervous system to influence the activity of other neurons or into the blood to act on other tissues. Through these targets, neuropeptides regulate a wide range of processes, which include development, feeding, growth, aggression, reproduction, and learning and memory (McShane et al. 1992;Park et al. 2003;Luquet et al. 2005;Crown et al. 2007;Nephew et al. 2009;Slaidina et al. 2009;Goncalves et al. 2012).One of the first genes identified to play a specific role in the development of peptidergic neurons was dimmed (dimm), which encodes a basic helix-loop-helix transcription factor that is required for the differentiation of diverse peptidergic neurons (Hewes et al. 2003;Park et al. 2008;Hamanaka et al. 2010). DIMM is a key regulator of expression of the neuropeptide biosynthetic enzyme, peptidylglycine-a-hydroxylating monooxygenase (PHM) (Park et al. 2008), and it promotes the differentiation of neurosecretory properties in many neurons (Hamanaka et al. 2010). Both DIMM and PHM are expressed widely and specifically in peptidergic neurons (Acampora et al. 1999;Michaud et al. 1998;Jiang et al. 2000;Hewes et al. 2003). In fact, DIMM was first identified by virtue of its pattern of peptidergic neuron expression through an enhancer-trap screen (Hewes et al. 2003). Similar expression pattern-based strategies may be useful for identification of other factors critical for peptidergic neuron de...

show abstract

Section: Identification Of Shep Through Splice-trap Screeningsupporting

confidence: 79%

Neuronal Remodeling During Metamorphosis Is Regulated by the alan shepard (shep) Gene in Drosophila melanogaster

Chen

Bjorum

et al. 2014

Genetics

View full text Add to dashboard Cite

show abstract

“…The Janus Kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays a dual role in promoting initial NE expansion in the OPC and inhibiting proneural wave progression (Yasugi et al, 2008;Ngo et al, 2010;Wang et al, 2011a;Wang et al, 2013). Loss of JAK/STAT signaling leads to loss of epithelial integrity, whereas over-activation of this pathway induces NE overgrowth (Wang et al, 2011a).…”

Section: Coordinated Actions Of Notch Egfr Jak/stat and Hippo/fat mentioning

confidence: 99%

“…Loss of JAK/STAT signaling leads to loss of epithelial integrity, whereas over-activation of this pathway induces NE overgrowth (Wang et al, 2011a). NE growth is mediated in part by the direct STAT target gene Nop56, a regulator of ribosome biogenesis and cell growth (Wang et al, 2013). In clones that are mutant for components of the JAK/STAT pathway, NE cells prematurely convert into NBs (Yasugi et al, 2008;Ngo et al, 2010;Wang et al, 2011a).…”

Section: Coordinated Actions Of Notch Egfr Jak/stat and Hippo/fat mentioning

confidence: 99%

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

Apitz¹,

Salecker²

2014

Journal of Neurogenetics

View full text Add to dashboard Cite

The brain areas that endow insects with the ability to see consist of remarkably complex neural circuits. Reiterated arrays of many diverse neuron subtypes are assembled into modular yet coherent functional retinotopic maps. Tremendous progress in developing genetic tools and cellular markers over the past years advanced our understanding of the mechanisms that control the stepwise production and differentiation of neurons in the visual system of Drosophila melanogaster. The postembryonic optic lobe utilizes at least two modes of neurogenesis that are distinct from other parts of the fly central nervous system. In the first optic ganglion, the lamina, neuroepithelial cells give rise to precursor cells, whose proliferation and differentiation depend on anterograde signals from photoreceptor axons. In the second optic ganglion, the medulla, the coordinated activity of four signaling pathways orchestrates the gradual conversion of neuroepithelial cells into neuroblasts, while a specific cascade of temporal identity transcription factors controls subtype diversification of their progeny.

show abstract

“…Our previous study showed that Treh mRNA is expressed in the optic lobe NEs [27]. To study the function of Treh , we knocked down Treh activity by RNA interference (RNAi) using either c768-Gal4 or c855a-Gal4 , two drivers that are active in the optic lobe NEs from the first instar stage onward [4], [18].…”

Section: Resultsmentioning

confidence: 99%

Trehalase Regulates Neuroepithelial Stem Cell Maintenance and Differentiation in the Drosophila Optic Lobe

et al. 2014

Self Cite

View full text Add to dashboard Cite

As one of the major hydrolases in Drosophila, trehalase (Treh) catalyzes the hydrolysis of trehalose into glucose providing energy for flight muscle activity. Treh is highly conserved from bacteria to humans, but little is known about its function during animal development. Here, we analyze the function of Treh in Drosophila optic lobe development. In the optic lobe, neuroepithelial cells (NEs) first divide symmetrically to expand the stem cell pool and then differentiate into neuroblasts, which divide asymmetrically to generate medulla neurons. We find that the knockdown of Treh leads to a loss of the lamina and a smaller medulla. Analyses of Treh RNAi-expressing clones and loss-of-function mutants indicate that the lamina and medulla phenotypes result from neuroepithelial disintegration and premature differentiation into medulla neuroblasts. Although the principal role of Treh is to generate glucose, the Treh loss-of-function phenotype cannot be rescued by exogenous glucose. Thus, our results indicate that in addition to being a hydrolase, Treh plays a role in neuroepithelial stem cell maintenance and differentiation during Drosophila optic lobe development.

show abstract

Evidence for Tissue-Specific JAK/STAT Target Genes inDrosophilaOptic Lobe Development

Cited by 29 publications

References 56 publications

Neuronal Remodeling During Metamorphosis Is Regulated by the alan shepard (shep) Gene in Drosophila melanogaster

Neuronal Remodeling During Metamorphosis Is Regulated by the alan shepard (shep) Gene in Drosophila melanogaster

A Challenge of Numbers and Diversity: Neurogenesis in theDrosophilaOptic Lobe

Trehalase Regulates Neuroepithelial Stem Cell Maintenance and Differentiation in the Drosophila Optic Lobe

Contact Info

Product

Resources

About