Not CD68 but stabilin‐1 expression is associated with the risk of recurrence in patients with oral cavity squamous cell carcinoma

Abstract

Background: This study's aim was to identify CD68 and stabilin-1 expressions and their prognostic implications in patients with oral cavity cancer (OCC). Methods: Stabilin-1 and CD68 immunochemical staining was conducted in samples from patients with OCC who underwent curative surgery in our institute. We retrospectively analyzed patients' clinical information. We investigated the relationship between CD68 and stabilin-1 expression levels and their effect on prognostic factors and survival. Results: Fifty-four patients' data were analyzed. CD68 and stabilin-1 expressions were positively correlated (P = .008). CD68 and stabilin-1 expressions were not correlated with other prognostic factors (eg, T classification or lymph node metastasis). Stabilin-1 expression and tumor recurrence risk were positively correlated (P = .015). CD68 expression was neither associated with recurrence-free nor OCC-specific survival. Conclusion: CD68 and stabilin-1 expressions in OCC were positively correlated. Stabilin-1 expression was significantly associated with OCC recurrence risk, but the prognostic value of CD68 was not confirmed. K E Y W O R D SCD68, oral cavity cancer, recurrence, stabilin-1, tumor-associated macrophage

CD68+ Macrophage Infiltration Associates With Poor Outcome of HPV Negative Oral Squamous Carcinoma Patients Receiving Radiation: Poly(I:C) Enhances Radiosensitivity of CAL-27 Cells but Promotes Macrophage Recruitment Through HMGB1

“…Some studies showed that high CD68 + macrophage associated with poor survival of OSCC ( 23 – 27 ) or with favorable survival of OSCC ( 28 ). Consistent with our results, there are also studies demonstrated no association of CD68 + macrophage infiltration with prognosis of OSCC patients ( 29 , 30 ). However, we observed a poor OS and a poor DFS in radiation treated OSCC patients with high CD68 + macrophage infiltration, especially in HPV negative subgroup, but not in HPV positive subgroup.…”

New tools to prevent cancer growth and spread: a ‘Clever’ approach

“…42 Similarly, in another cohort of patients with bladder cancer, intratumoural Clever-1 TAM density is an independent prognostic factor for poor overall survival, 43 and increases the risk of recurrence in patients with oral cavity cancers. 44 Gene expression analyses using multiple datasets have also identified high STAB1 expression as a prognostic factor for poor outcome in patients with cytogenetically normal acute myeloid leukaemia (CN-AML). 45 The same study shows that not only does Clever-1 suppression in AML cell lines (KG-1 and NB4) inhibit their proliferation, but it also makes them more sensitive to the BCL2 inhibitor, venetoclax, resulting in increased apoptosis of the cells.…”

“…This notion is also supported by data showing that targeting Clever-1 in combination with anti-PD-1 in refractory (immunologically cold, such as colon [CT26] and triple-negative breast [4T1]) tumour models improves treatment efficacy. 47 Relatively few reports have been published addressing Clever-1 expression as a predictive or prognostic factor for patient outcome, 40,41,43,44 and there are no reports of Clever-1 expression during ICI treatments; we, therefore, sought to investigate the significance of Clever-1 expression (at the mRNA level) in immunotherapy resistance, T-cell exclusion and dysfunction by extracting data from publicly available clinical datasets. Generally, a high expression of STAB1 in cancer patients of The Cancer Genome Atlas pan-cancer cohort (n = 11768) is significantly associated with reduced survival, regardless of patient characteristics, tumour type, therapy or clinical manifestations (Fig.…”

“… 42 Similarly, in another cohort of patients with bladder cancer, intratumoural Clever-1 TAM density is an independent prognostic factor for poor overall survival, 43 and increases the risk of recurrence in patients with oral cavity cancers. 44 …”

Prognostic Values of Stabilin-2 in Hepatocellular Carcinoma

“…However, in more advanced satges (Stage IV), patients with a high number of peritumoral or intratumoral Stabilin-1 macrophages showed a shorter disease-specific survival (p=0.05, and p=0.008, respectively) [28]. In addition, previous studies have reported that Stabilin-1 is associated with the prognosis of bladder urothelial carcinoma and oral squamous cell carcinoma [29,30]. Riabov V demonstrated that Stabilin-1 is expressed on tumor-associated macrophages (TAM) in human breast cancer, and with higher expression levels recorded in stage I [31].…”