Nosema adaliae sp. nov., a new microsporidian pathogen from the two-spotted lady beetle, Adalia bipunctata L. (Coleoptera: Coccinellidae) and its relationship to microsporidia that infect other coccinellids

Steele, T.; Bjørnson, S.

doi:10.1016/j.jip.2013.09.008

Cited by 19 publications

(7 citation statements)

References 32 publications

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“…Among the other microsporidians detected in our study, N. adaliae was found in Adalia bipunctata (Coleoptera) (Steele & Bjørnson, 2014), while N. pieriae , N. chrysorrhoeae , N. portugal and Nosema sp. CHW‐2007a were noted in different lepidopteran hosts (Huang et al., 2008; Hyliš et al., 2006; Maddox et al., 1999; Yaman, Bekircan, Radek, & Linde, 2014).…”

Section: Discussionmentioning

confidence: 70%

A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae)

Trzebny

Słodkowicz-Kowalska

Becnel

et al. 2020

Molecular Ecology Resources

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DNA metabarcoding offers new perspectives, especially with regard to the highthroughput identification and diagnostics of pathogens. Microsporidia are an example of widely distributed, opportunistic and pathogenic microorganisms in which molecular identification is important for both environmental research and clinical diagnostics. We have developed a method for parallel detection of both microsporidian infection and the host species. We designed new primer sets: one specific for the classical Microsporidia (targeting the hypervariable V5 region of small subunit [ssu] rDNA), and a second one targeting a shortened fragment of the COI gene (standard metazoan DNA-barcode); both markers are well suited for next generation sequencing. Analysis of the ssu rDNA data set representing 607 microsporidian species (120 genera) indicated that the V5 region enables identification of >98% species in the data set (596/607). To test the method, we used microsporidians that infect mosquitoes in natural populations. Using mini-COI data, all field-collected mosquitoes were unambiguously assigned to seven species; among them almost 60% of specimens were positive for at least 11 different microsporidian species, including a new microsporidian ssu rDNA sequence (Microsporidium sp. PL01). Phylogenetic analysis showed that this species belongs to one of the two main clades in the Terresporidia. We found a high rate of microsporidian co-infections (9.4%). The numbers of sequence reads for the operational taxonomic units suggest that the occurrence of Nosema spp. in co-infections could benefit them; however, this observation should be retested using a more intensive host sampling. Our results show that DNA barcoding is a rapid and cost-effective method for deciphering sample diversity in greater resolution, including the hidden biodiversity that may be overlooked using classical methodology.

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Section: Discussionmentioning

confidence: 70%

A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae)

Trzebny

Słodkowicz-Kowalska

Becnel

et al. 2020

Molecular Ecology Resources

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“…The microsporidium Nosema adaliae has recently been described from field-collected A. bipunctata (Steele & Bjørnson 2014), and because lady beetles are known to be susceptible to more than one species of microsporidia, Steele and Bjørnson (2012) examined the effects of two microsporidian pathogens on the fitness of A. bipunctata under laboratory conditions.…”

Section: Adalia Bipunctata (Coleoptera: Coccinellidae)mentioning

confidence: 99%

Microsporidia Biological Control Agents and Pathogens of Beneficial Insects

Bjørnson

2014

Microsporidia

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“…However, the relationship between the newly identified microsporidia and known pathogens will remain unclear until the genome sequence is available. The genus Nosema comprises 80 species (Kirk et al ., ) including some that are common in coccinellid hosts (Cali & Briggs, ; Saito & Bjørnson, ; Bjørnson et al ., ; Steele & Bjørnson, ). Several previous studies have identified 2 morphologically distinct microsporidial spore types (primary and environmental spores) with different functions (Weiser et al ., , ; Kohlmayr et al ., ).…”

Section: Microsporidia In the Coleopteramentioning

confidence: 99%

“…However, the use of microsporidia to control introduced pests is controversial (Solter & Maddox, 1988). Microsporidia are frequent parasites of ladybird beetles and the identification of novel species is far from complete (Table 1, listed in Bjørnson et al, 2011;Steele & Bjørnson, 2014). The release of ladybird species as biological control agents may have promoted the spread of microsporidia into new areas and potentially to new coccinellid hosts (Joudrey & Bjørnson, 2007;Bjørnson, 2008;Bjørnson et al, 2011).…”

Section: Microsporidia In the Coleopteramentioning

confidence: 99%

Evolutionary ecology of microsporidia associated with the invasive ladybird Harmonia axyridis

et al. 2014

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Invasive species are characterized by the rapid growth and spread of their populations after establishing a foothold in new habitats, and there are now many examples of such species negatively affecting biodiversity and the economy. It is unclear why some species can become successful invaders, whereas most (even if closely related) remain noninvasive. We previously proposed a hypothesis that parasites associated with invading species can promote their invasive success if they are harmless toward the invaders but harmful to their competitors and/or predators in the newly colonized habitat. Here we discuss whether microsporidia that have recently been discovered in the invasive ladybird Harmonia axyridis contribute to its invasive success. We show that all H. axyridis beetles sourced from diverse collection sites all over the world carry abundant microsporidia. This suggests that both native and invasive H. axyridis populations are associated with these tolerated parasites, which were likely to have existed in native populations before expansion rather than being acquired in newly colonized areas. We describe the pathogenesis of the microsporidia during different developmental stages of H. axyridis and we address the possibility that the predation of its infected eggs and larvae by competing native ladybird species may lead to their infection and ultimately to their decline. Finally, we discuss our initial hypothesis: microsporidia that are tolerated by an invasive vector insect can be active against susceptible native competitors and/or predator species.

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Nosema adaliae sp. nov., a new microsporidian pathogen from the two-spotted lady beetle, Adalia bipunctata L. (Coleoptera: Coccinellidae) and its relationship to microsporidia that infect other coccinellids

Cited by 19 publications

References 32 publications

A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae)

A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae)

Microsporidia Biological Control Agents and Pathogens of Beneficial Insects

Evolutionary ecology of microsporidia associated with the invasive ladybird Harmonia axyridis

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