Norovirus outbreaks in Beijing, China, from 2014 to 2017

Gao, Zhiyong; Liu, Baiwei; Yan, Huan; Li, Weihong; Jia, Lei; Tian, Yao; Chen, Yanwei; Wang, Quanyi; Pang, Xinghuo

doi:10.1016/j.jinf.2019.05.019

Cited by 31 publications

(37 citation statements)

References 35 publications

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“…Based on the genetic and sequence data, we conclude that the outbreak involved multiple genotypes, including GII.4[P4], GII.2[P16], and GII. 17[P17], which have been reported frequently in previous outbreaks [10]. These results reveal that the prevalent genotypes continue to infect humans.…”

Section: Discussionsupporting

confidence: 56%

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Full-genome sequences of GII.13[P21] recombinant norovirus strains from an outbreak in Changsha, China

et al. 2020

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On 31 March 2019, 68 school students suffered from vomiting, diarrhea, and abdominal pain after participating in a group activity at a commercial park. In this outbreak, multiple norovirus genotypes were observed, including GII.2[P16], GII.17[P17], and GII.13[P21]. Further, we determined the full-genome sequences of two strains of GII.13[P21] recombinant noroviruses, which were 7434 nt long. Phylogenetic analysis based on open reading frames (ORFs) 1 and 2 revealed that these recombinants were related to stains of different genotypes from different countries. The full genome nucleotide sequences of the two isolates were 97.0% and 98.0% identical to those of strains from London and Thailand, respectively. Simplot analysis revealed the presence of a break point at nt 5059 in the ORF1 region. The histo-blood group antigen binding sites were conserved in both recombinant viruses. Our findings not only provide valuable genetic information about a recombinant norovirus but also contribute to our general understanding of the evolution, genetic diversity, and distribution of noroviruses.

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Section: Discussionsupporting

confidence: 56%

“…The GII. 4[P4] genotype has been reported in many countries and is the prevalent strain in most outbreaks and human infections [9][10][11]. Previous reports have shown that GII.…”

Section: Introductionmentioning

confidence: 99%

Full-genome sequences of GII.13[P21] recombinant norovirus strains from an outbreak in Changsha, China

et al. 2020

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“…At least eight genotypes were identified during the study period, including GI.6[P11], GI. [30]. In Jinan, a city in eastern China, seven genotypes were prevalent in outbreaks of AGE from 2015 to 2017, while no GII.4 was identified [31].…”

Section: Discussionmentioning

confidence: 97%

An increasing prevalence of non-GII.4 norovirus genotypes in acute gastroenteritis outbreaks in Huzhou, China, 2014-2018

Chen

et al. 2020

Arch Virol

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Since 2014, novel non-GII.4 norovirus (NoV) genotypes continue to be reported as the main cause of outbreaks worldwide. In this study, we analyzed the epidemiological and genetic features of NoV outbreaks from July 2014 to June 2018 in Huzhou, China. A total of 450 stool samples collected from 51 AGE outbreaks were tested for NoVs by real-time RT PCR. Partial polymerase and capsid sequences of NoV-positive samples were amplified and sequenced for phylogenetic analysis. NoVs were found to be responsible of 84.3% of AGE outbreaks in Huzhou over the past 5 years. Most NoV outbreaks were reported in the cool months (November-March) and occurred in primary schools and kindergartens. Changes in the diversity of genotypes and the distribution of predominant types were observed in recent years. At least eight genotypes were identified, and 91.9% of the genotyped outbreaks were caused by non-GII.4 strains. The top three circulating genotypes during the study period were GII.2[P16], GII.3[P12], and GII.17[P17]. The predominant NoV genotypes in outbreaks have changed from GII.4 variants to GII.17[

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“…Molecular typing for HAdVs was also performed via conventional PCR using specific primers targeting the complete coding sequences of hexon, fiber and penton genes [25].Viral DNA was extracted from cultured medium with QIAamp RNA mini kits (Qiagen, Hilden, Germany) according to the manufacturer's instructions [26]. Conventional PCR was conducted using high-fidelity DNA polymerase (Takara, Dalian, China) according to the manufacturer's instructions.…”

Section: Hadv Isolation and Typingmentioning

confidence: 99%

An Outbreak of Acute Respiratory Illness at a Training Base in Beijing, China, Due to Human Adenovirus Type B55

Peng

et al. 2019

Preprint

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Background: An outbreak of acute respiratory illness (ARI) occurred at a training base in Beijing. A total of 12 students were diagnosed with symptoms of ARI from August 26 to August 30, 2015. The cause of the ARI outbreak was investigated. Methods: In partnership with the local center for disease control, we conducted an epidemiological investigation on the outbreak, collecting a total of twelve pharyngeal swab specimens as well as patient demographic for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate the causative virus, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber and penton genes of the isolated adenoviruses. In addition, we analyzed the whole genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students by multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven strains from the pharyngeal swab specimens. Genetic analysis showed that the coding sequences of the hexon, fiber, and penton genes obtained from those seven HAdV strains were identical, suggesting that they represented seven isolates of a single virus strain. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. Phylogenetic analysis showed that the genome of BJDX-01-2015 clustered with HAdV-B55 and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the culprit of the ARI outbreak. This was the first reported outbreak in Beijing due to HAdV-B55 virus. Continuous surveillance of respiratory adenoviruses is urgently needed for understanding the epidemiological and evolutionary features of HAdV-B55 and could also find value in an epidemiological modeling approach.

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Norovirus outbreaks in Beijing, China, from 2014 to 2017

Cited by 31 publications

References 35 publications

Full-genome sequences of GII.13[P21] recombinant norovirus strains from an outbreak in Changsha, China

Full-genome sequences of GII.13[P21] recombinant norovirus strains from an outbreak in Changsha, China

An increasing prevalence of non-GII.4 norovirus genotypes in acute gastroenteritis outbreaks in Huzhou, China, 2014-2018

An Outbreak of Acute Respiratory Illness at a Training Base in Beijing, China, Due to Human Adenovirus Type B55

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