Norovirus molecular detection in Uruguayan sewage samples reveals a high genetic diversity and GII.4 variant replacement along time

Victoria, Matías; Tort, Luis Fernando López; Lizasoain, Andrés; González-García, María J.; Castells, Matías; Berois, Mabel; Divizia, M; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira; Cristina, Juan; Colina, Rodney

doi:10.1111/jam.13058

Cited by 20 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The screening of sewage water for human viruses began with the detection and monitoring of polioviruses (Sinclair et al 2008;, and since then it has been successfully extended to study the circulation of different enteric and nonenteric viruses in the human population (La Rosa et al 2010Cantalupo et al 2011;Di Bonito et al 2014;Smith et al 2015;Victoria et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

et al. 2016

Self Cite

View full text Add to dashboard Cite

The prevalence and genetic diversity of human bocaviruses (HBoVs) in sewage water samples are largely unknown. In this study, 134 raw sewage samples from 25 wastewater treatment plants (WTPs) in Italy were analyzed by nested PCR and sequencing using species-specific primer pairs and broad-range primer pairs targeting the capsid proteins VP1/VP2. A large number of samples (106, 79.1 %) were positive for HBoV. Out of these, 49 were classified as HBoV species 2, and 27 as species 3. For the remaining 30 samples, sequencing results showed mixed electropherograms. By cloning PCR amplicons and sequencing, we confirmed the copresence of species 2 and 3 in 29 samples and species 2 and 4 in only one sample. A real-time PCR assay was also performed, using a newly designed TaqMan assay, for quantification of HBoVs in sewage water samples. Viral load quantification ranged from 5.51E+03 to 1.84E+05 GC/L (mean value 4.70E+04 GC/L) for bocavirus 2 and from 1.89E+03 to 1.02E+05 GC/L (mean value 2.27E+04 GC/L) for bocavirus 3. The wide distribution of HBoV in sewages suggests that this virus is common in the population, and the most prevalent are the species 2 and 3. HBoV-4 was also found, representing the first detection of this species in Italy. Although there is no indication of waterborne transmission for HBoV, the significant presence in sewage waters suggests that HBoV may spread to other water environments, and therefore, a potential role of water in the HBoV transmission should not be neglected.

show abstract

Section: Discussionmentioning

confidence: 99%

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite the lack of studies describing in depth the impact of NoV and HAdV in the health of Uruguayan children, all the above-mentioned viruses have been reported as acute gastroenteritis causing agents in Uruguayan paediatric population, with incidences according the expected for RVA (37%) and HAstV (10%). Moreover, a wide dispersion as well as a wide genetic diversity in the Uruguayan environment has been recently reported for these viral pathogens (Victoria et al 2014(Victoria et al , 2016Lizasoain et al 2015;Tort et al 2015;Varela et al 2015;Lopez et al 2017). This information raises the need to evaluate the wastewater treatment process existing in the country to assure viral removal from wastewater and reach effluent of good quality.…”

Section: Introductionmentioning

confidence: 97%

“…Moreover, a wide dispersion as well as a wide genetic diversity in the Uruguayan environment has been recently reported for these viral pathogens (Victoria et al . , ; Lizasoain et al . ; Tort et al .…”

Section: Introductionmentioning

confidence: 99%

Human enteric viruses in a wastewater treatment plant: evaluation of activated sludge combined with UV disinfection process reveals different removal performances for viruses with different features

Lizasoain

Tort

González-García

et al. 2018

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent environmental surveillance studies revealed that human noroviruses in the environment are sometimes more genetically diverse than are outbreak strains, suggesting that the genotype distribution of noroviruses associated with sporadic or asymptomatic infections is higher. In sewage samples, the proportion of GI versus GII noroviruses is often similar (30, 62), which could also indicate different survival characteristics for GI and GII viruses.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Human Noroviruses in Commercial Food Establishment Bathrooms

Leone

Dharmasena

Tang

et al. 2018

Journal of Food Protection

View full text Add to dashboard Cite

Although transmission of human norovirus in food establishments is commonly attributed to consumption of contaminated food, transmission via contaminated environmental surfaces, such as those in bathrooms, may also play a role. Our aim was to determine the prevalence of human norovirus on bathroom surfaces in commercial food establishments in New Jersey, Ohio, and South Carolina under nonoutbreak conditions and to determine characteristics associated with the presence of human norovirus. Food establishments (751) were randomly selected from nine counties in each state. Four surfaces (underside of toilet seat, flush handle of toilet, inner door handle of stall or outer door, and sink faucet handle) were swabbed in male and female bathrooms using premoistened macrofoam swabs. A checklist was used to collect information about the characteristics, materials, and mechanisms of objects in bathrooms. In total, 61 (1.5%) of 4,163 swabs tested were presumptively positive for human norovirus, 9 of which were confirmed by sequencing. Some factors associated with the presence of human norovirus included being from South Carolina (odd ratio [OR], 2.4; 95% confidence interval [CI], 1.2 to 4.9; P < 0.05) or New Jersey (OR, 1.7; 95% CI, 0.9 to 3.3; 0.05 < P < 0.10), being a chain establishment (OR, 1.9; 95% CI, 1.1 to 3.3; P < 0.05), being a unisex bathroom (versus male: OR, 2.0; 95% CI, 0.9 to 4.1; 0.05 < P < 0.10; versus female: OR, 2.6; 95% CI, 1.2 to 5.7; P < 0.05), having a touchless outer door handle (OR, 3.3; 95% CI, 0.79 to 13.63; 0.05 < P < 0.10), and having an automatic flush toilet (OR, 2.5, 95% CI, 1.1 to 5.3; 0.05 < P < 0.10). Our findings confirm that the presence of human norovirus on bathroom surfaces in commercial food establishments under nonoutbreak conditions is a rare event. Therefore, routine environmental monitoring for human norovirus contamination during nonoutbreak periods is not an efficient method of monitoring norovirus infection risk.

show abstract

Norovirus molecular detection in Uruguayan sewage samples reveals a high genetic diversity and GII.4 variant replacement along time

Abstract: This approach can be used as an indicator of the presence of a new GII.4 variant which can originate an increase in acute gastroenteritis outbreaks worldwide.

Cited by 20 publications

References 31 publications

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

Human enteric viruses in a wastewater treatment plant: evaluation of activated sludge combined with UV disinfection process reveals different removal performances for viruses with different features

Prevalence of Human Noroviruses in Commercial Food Establishment Bathrooms

Contact Info

Product

Resources

About