Normoglycemia after reflux of islet-containing pancreatic fragments into the splenic vascular bed in dogs

Warnock, Garth L.; Rajotte, Ray V.; Procyshyn, A. W.

doi:10.2337/diabetes.32.5.452

Cited by 28 publications

(19 citation statements)

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“…Others have found that injection of dispersed hepatocytes into dog spleen was followed by a small transient increase of ASAT. Similar observations have been made after intraportal islet transplantation in rats and dogs [10]. This was not observed during hepatocyte transplantation into the pancreas [14] but the proportion of hepatocytes surviv ing in the early posttransplantation period is probably higher by intrasplenic transplanta tion.…”

Section: Discussionsupporting

confidence: 72%

Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Briand¹,

Centeno²,

Astre³

et al. 1993

Eur Surg Res

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Autologous hepatocellular transplantation using reflux into the spleen via the venous tributaries was compared in 65% hepatectomized dogs to direct injection into the splenic pulp. Transplantation into the spleen via the inferior terminal polar splenic vein led to a better survival rate (50 vs. 0%). Hepatocytes embolization to the intrahepatic portal vein system was observed. Although hepatocyte transplantation into the spleen can be accomplished either by direct punction of the spleen or by retrograde injection into a splenic vein, the second method decreases the frequency of cell embolization.

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Section: Discussionsupporting

confidence: 72%

Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Briand¹,

Centeno²,

Astre³

et al. 1993

Eur Surg Res

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“…The site of splenic injury was closed with a pursestring suture. The prepared tissue of group 2 dogs was injected through a 19-gauge needle into the trabecular system of the spleen by reflux into the splenic venous tributaries as described by Warnock et al, 16 who used the Horaguchi technique. The punctured veins were ligated thereafter.…”

Section: Implantation Techniquementioning

confidence: 99%

Comparison of Two Methods of Islet Preparation and Transplantation in Dogs

et al. 1986

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Seventy-nine mongrel dogs underwent total pancreatectomy. Fifteen dogs served as apancreatic controls and died 7.0 +/- 4.2 days later (mean +/- SD). The pancreases of 44 dogs (group 1) were intraductally distended by manual injection of Hanks' balanced salt solution (HBSS). Thereafter each organ was mechanically disrupted and subjected to collagenase digestion as described by Mirkovitch et al. The pancreases of 20 dogs (group 2) were intraductally distended and subsequently perfused with collagenase by a roller pump. The organs were then mechanically disrupted and filtered through screens as described by Horaguchi et al. The resulting tissue suspensions were injected into the spleens of the dogs as autotransplants in both groups, by direct punction of the splenic capsule in group 1 and by retrograde infusion via a splenic vein tributary in group 2. The functional outcome was better in group 2 than in group 1, as assessed by the number of animals that became normoglycemic after transplantation [15/20 (75%) vs. 13/44 (30%); P = .0025]. The degree of islet purification, as measured by an increase in the tissue insulin/amylase ratio, was higher in group 2, and in both groups it was higher in normoglycemic than in hyperglycemic animals. The percent engraftment [i.e., amount of insulin recovered from spleen as percent of tissue transplanted (mean, 15.4% in group 1 and 14.5% in group 2) or as percent of original pancreas (mean, 4.9% in group 1 and 4.4% in group 2)] was low in both groups but again was higher in normoglycemic than in hyperglycemic animals within each group. In conclusion, both the degree of engraftment and purification and the route of implantation influenced the functional outcome after dispersed pancreatic islet autotransplantation to the spleen of totally pancreatectomized dogs, with purified tissue injected retrogradely functioning better than unpurified tissue injected directly.

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“…Numerous studies have been conducted using such preparations of islets in dogs after either direct injections of the splenic pulp (2, [4][5][6][7][8] or after retrograde reflux into splenic venous tributaries (9,10). Although there have been a few citations (7)(8)(9) of long-term functional survival of islet autografts prepared by these techniques, continuing islet autograft function has not yet been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

“…Although there have been a few citations (7)(8)(9) of long-term functional survival of islet autografts prepared by these techniques, continuing islet autograft function has not yet been studied in detail. Also, islet preparations by these techniques have not usually been transplanted into the portal venous system due to the threat of inducing portal hypertension or other complications associated with exocrine secretions (1 [1][2][3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Natural history of intrahepatic canine islet cell autografts.

Alejandro¹,

Cutfield²,

Shienvold³

et al. 1986

J. Clin. Invest.

143

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We have serially followed the function of intrahepatic canine islet autografts in 15 beagle dogs for up to 24 mo. Of these, only 20% sustained normal levels of fasting blood glucose for >15 mo posttransplant. Failure of autograft function was accompanied by a preferential loss of well-granulated beta cells in the engrafted islets. The chronic stimulation of an initially marginal intrahepatic beta-cell mass ultimately resulted in metabolic deterioration and loss of beta cells below the minimal threshold required to maintain normal fasting blood glucose levels. It is possible that transplantation of a larger mass of islets would result in indefinite graft function in dogs. However, it remains to be demonstrated in larger mammals, including humans, whether an islet cell mass that is initially adequate in a heterotopic site such as the liver can remain functionally competent over a prolonged period.

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Normoglycemia after reflux of islet-containing pancreatic fragments into the splenic vascular bed in dogs

Cited by 28 publications

References 0 publications

Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Comparison of Two Methods of Islet Preparation and Transplantation in Dogs

Natural history of intrahepatic canine islet cell autografts.

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