Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Briand, Dominique; Centeno, N.A.; Astre, C.; Aubert, Bernadette; Joyeux, H

doi:10.1159/000129264

Cited by 12 publications

(2 citation statements)

References 8 publications

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“…However, intraperitoneally injected bioencapsulated stem cells are dispersed throughout the peritoneal cavity and not localized, and this may irritate the peritoneal lining to cause peritonitis. Spleen is a possible site for cell transplantation in the treatment of various liver diseases [10–13], especially when the liver is not suitable as a transplantation site. However, intrasplenically transplanted cells could migrate to the liver via the portal vein and cause embolism in the intrahepatic portal vein system [13]; therefore, in the present study, we bioencapsulated MSCs using alginate polylysine alginate (APA) membrane and transplanted them into the spleens of 90% hepatectomized rats.…”

Section: Introductionmentioning

confidence: 99%

Intrasplenic Transplantation of Bioencapsulated Mesenchymal Stem Cells Improves the Recovery Rates of 90% Partial Hepatectomized Rats

Liu

Chang

2012

Stem Cells International

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Mesenchymal stem cells (MSCs) derived from bone marrow can secrete cytokines and growth factors and can transdifferentiate into liver cells. We transplanted polymeric membrane bioencapsulated MSCs into the spleens of 90% partial hepatectomized rats. This resulted in 91.6% recovery rates. This is compared to a recovery rate of 21.4% in the 90% hepatectomized rats and 25% in the 90% hepatectomized rats receiving intrasplenic transplantation of free MSCs. After 14 days, the remnant livers in the bioencapsulated MSCs group are not significantly different in weight when compared to the sham control group. From day 1 to day 3 after surgery, in the bioencapsulated MSCs group, the plasma HGF and IL-6 were significantly higher than those in the free MSCs group and control group (P < 0.01); plasma TNF-α was significantly lower (P < 0.001). We concluded that the intrasplenic transplantation of bioencapsulated MSCs significantly increases the recovery rates of 90% hepatectomized rats. It is likely that the initial effect is from proliver regeneration factors followed later by the transdifferentiated hepatocyte-like cells. However, histopathological analysis and hepatocyte proliferation study will be needed to better understand the regenerative mechanisms of this result. This study has implications in improving the survival and recovery of patients with very severe liver failure due to hepatitis, trauma, or extensive surgical resection.

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Section: Introductionmentioning

confidence: 99%

Intrasplenic Transplantation of Bioencapsulated Mesenchymal Stem Cells Improves the Recovery Rates of 90% Partial Hepatectomized Rats

Liu

Chang

2012

Stem Cells International

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“…This route has been used to administer hepatocytes for transplantation (9,10), and has been used to establish diffuse liver metastases (11,12). We have used this model to show that treatment of diffuse liver metastases in mice with a single portal venous injection of an oncolytic Herpes simplex virus-1 (HSV-1) mutant results in a reduction in liver tumor burden, and prolongation of mouse survival (8,13,14).…”

Section: Introductionmentioning

confidence: 99%

Mouse Models of Subcutaneous Spleen Reservoir for Multiple Portal Venous Injections to Treat Liver Malignancies

et al. 2005

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Pharmacokinetic parameters of antipyrine in dog after hepatectomy

Galtier¹,

Briand

Pinguet

et al. 1995

Biopharm & Drug Disp

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Pharmacokinetic studies with antipyrine were carried out on beagle dogs to determine the consequence of hepatectomy on hepatic drug metabolizing capacity, the rate of hepatic regeneration, and the possible beneficial effect of hepatocellular transplantation. The drug (250 mg) was administered by short IV infusion in three groups of dogs (first group, 65% hepatectomy; second group, 65% hepatectomy with hepatocyte transplantation; third group 80% hepatectomy). Pharmacokinetic parameters of antipyrine were evaluated before surgery and within 10 d after surgery. Blood samples were taken at frequent intervals after drug administration and antipyrine was assayed in plasma by a specific HPLC method with UV detection. Before surgery, the mean elimination half-life was about 1.1 h and total clearance averaged 6 L h-1. In dogs with 65% hepatectomy, no statistical differences in pharmacokinetic parameters of antipyrine appeared before or after surgery. When 65% hepatectomy was associated with hepatocyte transplantation, a significant increase in elimination half-life and a significant decrease in total clearance were observed. The same statistical differences in the pharmacokinetic parameters were observed in the group with 80% hepatectomy. Transplantation of isolated hepatocytes into the spleen did not correct hepatocellular insufficiency. In this study, numerous laboratory tests were performed. A significant correlation was found between serum albumin, cholesterol, factor V, ALAT, total bilirubin, and ratio of amino acids and the pharmacokinetic parameters of antipyrine.

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Comparison of Two Methods of Autologous Intrasplenic Hepatocellular Transplantation in Partially Hepatectomized Dogs

Cited by 12 publications

References 8 publications

Intrasplenic Transplantation of Bioencapsulated Mesenchymal Stem Cells Improves the Recovery Rates of 90% Partial Hepatectomized Rats

Intrasplenic Transplantation of Bioencapsulated Mesenchymal Stem Cells Improves the Recovery Rates of 90% Partial Hepatectomized Rats

Mouse Models of Subcutaneous Spleen Reservoir for Multiple Portal Venous Injections to Treat Liver Malignancies

Pharmacokinetic parameters of antipyrine in dog after hepatectomy

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