NOR expression increases on metaphase chromosomes of down syndrome lymphocytes in concordance with mitogen concentration in culture medium

İmamoğlu, Nalan; Demirtaş, Halil; Dönmez-Altuntaş, Hamiyet; Hamurcu, Zühal; Ilten, Ali

doi:10.1002/cyto.b.20050

Cited by 13 publications

(16 citation statements)

References 17 publications

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“…Although the value of RNA contents in nonstimulated (11) and stimulated PBMC as given in this study is higher in infants and children with DS, these subjects suffer nonetheless from immune deficiency. In fact, RNA excess in the cells of DS infants compared to controls appears unnecessary/useless, even proposed to be harmful because of the wasted energy in producing unnecessary RNA transcript (9).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, NOR bearing chromosome number of metaphase lymphocytes from babies/children with DS were higher compared to those of the controls (9). These results do not prove however whether or not the mean rRNA/RNA level in cells of young DS patients was different than those of healthy controls, since the AgNOR staining is related with NORproteins and NOR proteins level cannot be taken at the present as a direct indicator of RNA/rRNA content in DS cells (until proving that the increase in NOR proteins level is numerically parallel to that of the RNA/rRNA level).…”

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confidence: 82%

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Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

Hamurcu

Demirtaş

Patıroğlu

et al. 2006

Cytometry Part B Clinical

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Objective: The aim of the present study was to determine whether or not the phytohemagglutinin (PHA)-activated proliferation and average RNA content in peripheral blood mononuclear cells (PBMC) of Down syndrome (DS) patients change with age.Method: Stimulated portion of PBMC and total RNA levels in these cells after 72 h of PHA stimulation from 38 DS patients were compared with 28 age-matched healthy controls using flow cytometric measurement.Results: Decreased ratio of PBMC from DS patients undergoes mitogenic stimulation with age (r = À0.84, P = 0.000). This decrease is not observed in the cells of control individuals (r = 0.03, P = 0.869). Stimulated PBMC in infants with DS have higher level of RNA contents compared to controls (Z = 2.227, P = 0.026). While RNA content in mitogen-stimulated PBMC of DS decreased progressively and significantly with age (r = À0.70, P = 0.000), no significant age-related change in RNA content was found among the cells of healthy individuals in the range of 0-27 year old (r = 0.275, P = 0.157, P > 0.05).Conclusion: Age-dependent decreases in mitogen-activated proliferation ratio and average RNA content of PBMC from DS patients appear as regular events. These results may contribute to the explanation of the immune deficiency seen in DS patients since the PHA-stimulated cells are principally T-lymphocytes. This is the first report on the decrease in PHA-stimulated proliferation ratio (stimulability) and RNA level in PBMC of DS patients in relation to age. q 2006 Clinical Cytometry Society

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Section: Discussionmentioning

confidence: 99%

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confidence: 82%

Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

Hamurcu

Demirtaş

Patıroğlu

et al. 2006

Cytometry Part B Clinical

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“…However, few data are available from the studies on the relation between nucleolus organizer regions' (NORs) expression and DS phenotypes (1,2). The regulation of NORs expression in DS patients' cells, including the NOR situated on the extra chromosome 21 is not fully explained (3). The Down syndrome (DS) region on chromosome 21, which is responsible for the DS main features, has been defined by the analysis of DS patients with partial trisomy 21 (4) and transchromosomal mice (5).…”

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confidence: 99%

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

Hamurcu

Demirtaş

Kumandaş

2005

Cytometry Part B Clinical

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Objective: Trisomy 21 or Down syndrome (DS) is the most common genetic cause of mental retardation associated with the immunologic and other known defects. Extra chromosome 21 of DS patients contains an average of 40 extra copies of rRNA genes and the in vivo regulation of these genes' activity is not known. Because over 80% of total cellular RNA is rRNA, the measurement of total cellular RNA provides information on rRNA content. The aim of this work was to determine whether or not the additional chromosome 21 causes any increase in total cellular RNA content in mononuclear cells from peripheral blood (PBMNCs) of these patients and whether or not this content is modified with age.Method: PBMNCs of 48 patients with DS and 48 healthy controls were studied. RNA content of isolated PBMNCs was evaluated by flow cytometric measurements.Results: Average RNA content of younger DS patients' cells was significantly higher than that of healthy controls (P = 0.003). Furthermore, the RNA content decreased significantly with increasing age of DS patients (r = -0.377, P = 0.008) in the range of 0-26 year old, whereas no significant relationship was found between age and PBMNCs' RNA content of healthy controls in the same range of ages.Conclusion: RNA content of PBMNCs from DS patients decreases rapidly with age. This is the first work on the age-dependent decrease of the RNA content in PBMNCs of DS patients. q 2005 International Society for Analytical Cytology

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“…Those regions can be stained with silver, when they are active. Due to their silver affi nity, these proteins are called as argyrophilic NOR (AgNOR)-associated proteins and silver staining method is the most confident to indicate nucleoli in interphase nuclei (12) and to identify active NOR-bearing chromosomes at metaphase (13). There are various studies about the importance of the interphase quantity of AgNOR in tumor pathology, for the prognostic and diagnostic description of different cancer types (14,15).…”

Section: Introductionmentioning

confidence: 99%

The detection of curcumins’ antitumoral effects via argyrophilic nucleolar organizing region-associated protein synthesis in mice with ehrlich’s ascitic carcinoma

Nisari¹,

Yılmaz²,

Eröz³

et al. 2017

BLL

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BACKGROUND: Curcumin is a polyphenol compound that has antioxidant, anticancer, anti-infl ammatory, antihyperlipidemic and antimicrobial effects. Nucleolar-organizing regions are the sites of the gene on chromosomes. The present study was aimed to show the antitumoral effect of curcumin via AgNOR protein synthesis in Ehrlich's ascitic carcinoma (EAC) bearing mice. METHODS: Twenty three mice with EAC were randomly divided into 3 groups as positive control (n = 7), group 2 (n = 8) and 3 (n = 8) treated intraperitoneally with curcumin (25 mg/kg) and (50 mg/kg), respectively. The animals were sacrifi ced on Day 16, the solid tumors were removed out. Then, total AgNOR area/nuclear area (TAA/NA) and the mean AgNOR number were estimated for each mice. RESULT: Statistically signifi cant differences were determined among the whole groups for TAA/NA ratio (p = 0.000), conversely mean AgNOR number (p = 0.361). When comparingthe two groups; while no difference was determined between the control and curcumin (25 mg/kg) groups (p = 0.061), the signifi cant differences were detected between the control and curcumin (50 mg/kg) groups (p = 0.000) and between curcumin (25 mg/kg) and curcumin (50 mg/kg) groups (p = 0.000) for TAA/NA ratio. However, there was no signifi cant difference for the mean AgNOR number in double comparison of the groups. CONCLUSIONS: The current study showed that curcumin had a crucial function against cancer development. Also, both AgNOR values might be used as biomarkers for detection of the most reliable therapeutic dose selection of cancer treatment (Tab. 3, Fig. 2, Ref. 27). Text in PDF www.elis.sk.

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NOR expression increases on metaphase chromosomes of down syndrome lymphocytes in concordance with mitogen concentration in culture medium

Cited by 13 publications

References 17 publications

Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

The detection of curcumins’ antitumoral effects via argyrophilic nucleolar organizing region-associated protein synthesis in mice with ehrlich’s ascitic carcinoma

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