The detection of curcumins’ antitumoral effects via argyrophilic nucleolar organizing region-associated protein synthesis in mice with ehrlich’s ascitic carcinoma

Abstract

BACKGROUND: Curcumin is a polyphenol compound that has antioxidant, anticancer, anti-infl ammatory, antihyperlipidemic and antimicrobial effects. Nucleolar-organizing regions are the sites of the gene on chromosomes. The present study was aimed to show the antitumoral effect of curcumin via AgNOR protein synthesis in Ehrlich's ascitic carcinoma (EAC) bearing mice. METHODS: Twenty three mice with EAC were randomly divided into 3 groups as positive control (n = 7), group 2 (n = 8) and 3 (n = 8) treated intraperitoneally with curcumin (25 mg/kg) and (50 mg/kg), respectively. The animals were sacrifi ced on Day 16, the solid tumors were removed out. Then, total AgNOR area/nuclear area (TAA/NA) and the mean AgNOR number were estimated for each mice. RESULT: Statistically signifi cant differences were determined among the whole groups for TAA/NA ratio (p = 0.000), conversely mean AgNOR number (p = 0.361). When comparingthe two groups; while no difference was determined between the control and curcumin (25 mg/kg) groups (p = 0.061), the signifi cant differences were detected between the control and curcumin (50 mg/kg) groups (p = 0.000) and between curcumin (25 mg/kg) and curcumin (50 mg/kg) groups (p = 0.000) for TAA/NA ratio. However, there was no signifi cant difference for the mean AgNOR number in double comparison of the groups. CONCLUSIONS: The current study showed that curcumin had a crucial function against cancer development. Also, both AgNOR values might be used as biomarkers for detection of the most reliable therapeutic dose selection of cancer treatment (Tab. 3, Fig. 2, Ref. 27). Text in PDF www.elis.sk.

Effects of Curcumin on Tobacco Smoke-induced Hepatic MAPK Pathway Activation and Epithelial-Mesenchymal TransitionIn Vivo

“…In addition, an appropriate increase in the use of dose is also a conventional method. Curcumin (50 or 100 mg/kg BW) has been shown to attenuate diseases, EMT and tumor process in animal studies (Afrin et al, ; Goncalves Vde et al, ; Momeni and Eskandari, ; Nisari et al, ; Yuan et al, ). Studies from our laboratory have also suggested that 100 mg/kg BW curcumin restored tobacco smoke‐induced EMT in the mice stomach and bladder tissues.…”

The investigation of the antitumoral effect of Cornus mas L in mice with ehrlich solid tumor

“…It is important to identify new biomarkers to differentiate benign and malignant lesions. In addition, the selection of the most reliable therapeutic strategy for cancer treatment is very important for the management of the treatment strategy and to improve success (16,18,36). We aimed to determine the average number of AgNOR in our study.…”

“…These regions are composed of ribosomal DNA (rDNA) and some proteins with argyrophilic properties. These regions are copied to the ribosomal RNA, which is transformed into the immature ribosomes in the nucleolus and mature ribosomes in the cytoplasm, respectively (16). These regions can be stained with silver, when active.…”

“…These regions can be stained with silver, when active. Depending on the silver, these proteins are called argyrophilic NOR (AgNOR) related proteins, and the silver staining method is the most suitable to indicate nucleoli in the interphase nuclei (16,17). There are several studies on the importance of interphase AgNOR quantifi cation in tumor pathology for prognostic and diagnostic defi nitions of different types of cancer (18).…”

Experimental Cancer Models

“…In experimental studies, experimental animals are frequently used for clinical observations [7,8]. In animal experiments for cancer treatments, often plant extracts have an important place in research [9][10][11].…”