Nonwoven Scaffolds of Improved Design for the Tissue Engineering of the Anterior Cruciate Ligament

Edwards, Sarah C.; Russell, Stephen J.; Ingham, Eileen; Matthews, J.B.; Mitchell, W. L.

doi:10.1533/9781845694104.6.355

Cited by 19 publications

(18 citation statements)

References 16 publications

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“…A substrate affects the production of extracellular matrix components by the cells, its synthesis, and composition. The ability to maintain cellular adhesion and proliferation is considered to be an important in vitro biocompatibility parameter for a material used as a substrate [10-12]. Hence, a material with inhibiting properties will inhibit tissue regeneration in vivo .…”

Section: Resultsmentioning

confidence: 99%

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

et al. 2014

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Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.

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Section: Resultsmentioning

confidence: 99%

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

et al. 2014

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“…The knee articular chondrocytes stopped growing earlier than nasal septum chondrocytes because of the aging process as they underwent multiple passage. According to Edward et al (2004) and Wiedmann et al (2002), the attachment of the cells on the scaffold will be influenced by the scaffold structure and cell-substrate biocompatibility leading to subsequent cell spreading, cell migration and often cell differentiation function. In our study, the SEM showed both chondrocytes grew well on the surface and pores of the bovine scaffold.…”

Section: Discussionmentioning

confidence: 99%

The microscopic biological response of human chondrocytes to bovine bone scaffold

et al. 2008

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This study was performed to determine the microscopic biological response of human nasal septum chondrocytes and human knee articular chondrocytes placed on a demineralized bovine bone scaffold. Both chondrocytes were cultured and seeded onto the bovine bone scaffold with seeding density of 1 x 105 cells per 100 microl/scaffold and incubated for 1, 2, 5 and 7 days. Proliferation and viability of the cells were measured by mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed by scanning electron microscopy and differentiation study was analyzed by immunofluorescence staining and confocal laser scanning electron microscopy. The results showed good proliferation and viability of both chondrocytes on the scaffolds from day 1 to day 7. Both chondrocytes increased in number with time and readily grew on the surface and into the open pores of the scaffold. Immunofluorescence staining demonstrated collagen type II on the scaffolds for both chondrocytes. The results showed good cells proliferation, attachment and maturity of the chondrocytes on the demineralized bovine bone scaffold. The bovine bone being easily resourced, relatively inexpensive and non toxic has good potential for use as a three dimensional construct in cartilage tissue engineering.

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“…For example, a change in the chain conformation in nylon 6 and nylon 12 due to electrospinning occurs, implying that a high stress is induced on the electrospinning jet as the fi bres are being formed, and this stress alters the chain conformation of the nylon backbone [47]. Nonwoven scaffolds based on the PET, PGA and PLA have been developed for tissue engineering of the anterior cruciate ligament and cartilage [50,51]. The electrospun PET NFM was fi rst treated in formaldehyde to yield hydroxyl groups on the surface, followed by the grafting polymerization of methacrylic acid (MAA) initiated by Ce(IV).…”

Section: Nonwovenmentioning

confidence: 99%

Polyester and nylon based textiles in biomedical engineering

Gupta

Grover

Viju

et al. 2008

Polyesters and Polyamides

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Nonwoven Scaffolds of Improved Design for the Tissue Engineering of the Anterior Cruciate Ligament

Cited by 19 publications

References 16 publications

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

The microscopic biological response of human chondrocytes to bovine bone scaffold

Polyester and nylon based textiles in biomedical engineering

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