Nonviral genetic transfer of Fas ligand induced significant growth suppression and apoptotic tumor cell death in prostate cancer in vivo

Nakanishi, Hiroyuki; Mazda, Osam; Satoh, Etsuko; Asada, Hidetsugu; Morioka, Hiroyuki; Kishida, Tsunao; Nakao, Masahiro; Mizutani, Yoichi; Kawauchi, Akihiro; Kita, Masakazu; Imanishi, Jirô; Miki, Tsuneharu

doi:10.1038/sj.gt.3301912

Cited by 61 publications

(37 citation statements)

References 48 publications

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“…The plasmids pGEG.GL3 [28], pGE-G.EGFP [29], and pGEG.b [30] carried GL3 firefly luciferase (Luc), EGFP, and Escherichia coli b-galactosidase (b-gal) genes, respectively, under the control of the CAG promoter. Each plasmid also contained Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and EBV oriP sequence [31].…”

Section: Methodsmentioning

confidence: 99%

Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart

Tsunoda

Mazda

Oda

et al. 2005

Biochemical and Biophysical Research Communications

129

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Naked plasmid DNA (pDNA) and short interfering RNA (siRNA) duplexes were transduced into adult murine heart by means of sonoporation using the third-generation microbubble, BR14. Plasmid DNAs carrying luciferase, b-galactosidase (b-gal), or enhanced green fluorescent protein (EGFP) reporter genes were mixed with BR14 and injected percutaneously into the left ventricular (LV) cavity of C57BL/6 mice while exposed to transthoracic ultrasound at 1 MHz for 60 s. Sonoporation at an output intensity of 2.0 W/cm 2 and a 50% pulse duty ratio resulted in the highest luciferase expression in the heart. Histological examinations revealed significant expression of the b-gal and EGFP reporters in the subendocardial myocardium of LV. Intraventricular co-injection of siRNA-GFP and BR14 with concomitant ultrasonic exposure resulted in substantial reduction in EGFP expression in the coronary artery in EGFP transgenic mice. The present method may be applicable to gain-of-function and loss-of-function genetic engineering in vivo of adult murine heart.

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Section: Methodsmentioning

confidence: 99%

Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart

Tsunoda

Mazda

Oda

et al. 2005

Biochemical and Biophysical Research Communications

129

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“…70 Briefly, triplicate aliquots of cells were cultured in 96-well plates under standard conditions followed by addition of 20 ml of 0.5 mmol of 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST8; Nacalai Tesque). After incubation for an additional 2 h, 100 ml of the supernatant was collected to measure the OD at 450 nm.…”

Section: Cell Viabilitymentioning

confidence: 99%

Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf

et al. 2006

Self Cite

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Microphthalmia-associated transcription factor (Mitf) is critically involved in melanin synthesis as well as differentiation of cells of the melanocytic lineage. Some earlier studies suggested that Mitf is also essential in the survival of melanoma cells, but this notion remains controversial. We synthesized short interfering RNA (siRNA) duplexes corresponding to the mitf sequence and transfected them into B16 melanoma. Lipid-mediated transfection in vitro of Mitfspecific siRNA resulted in specific downregulation of Mitf and of the tyrosinase that is a transcriptional target of Mitf. This treatment also remarkably reduced the viability of melanoma cells by inducing apoptosis. To examine the potential feasibility of RNAi therapy against melanoma, B16 cells were subcutaneously injected into syngenic mice and siRNA was transfected into the pre-established tumor by means of electroporation. The Mitf-specific siRNA drastically reduced outgrowth of subcutaneous melanoma, while nonspecific siRNA failed to affect tumor progression. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling-based analysis of tumor specimens demonstrated that the tumor cells transfected with Mitf-siRNA effectively underwent apoptosis in vivo. The present results indicate that Mitf plays important roles in melanoma survival. Intratumor electrotransfer of Mitf-specific siRNA may provide a powerful strategy for therapeutic intervention of malignant melanoma.

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“…Considering that apoptosis plays a central role in regulation of pituitary tissue homeostasis, the imbalance between cell death and proliferation in favor of cell survival could result in tumor formation. One of the commonly employed strategies in experimental gene therapy for cancer is to target death receptors specifically to trigger apoptosis in tumor cells (33)(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

RGD-FasL Induces Apoptosis of Pituitary Adenoma Cells

Chen

Zhuang

et al. 2008

Cell Mol Immunol

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Nonviral genetic transfer of Fas ligand induced significant growth suppression and apoptotic tumor cell death in prostate cancer in vivo

Cited by 61 publications

References 48 publications

Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart

Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart

Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf

RGD-FasL Induces Apoptosis of Pituitary Adenoma Cells

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