RGD-FasL Induces Apoptosis of Pituitary Adenoma Cells

Chen, Lukui; Zhuang, Guohong; Li, Wenzhu; Liu, Yunsheng; Zhang, Junqing; Tian, Xin

doi:10.1038/cmi.2008.8

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…In cells sensitive to Fas-mediated apoptosis, caspase-8 is autoprocessed to generate the active form that can cleave downstream effector caspases, including caspase-3. Active caspase-8 is capable of unleashing cascades of cellular events that can result in apoptosis [32]. Activation of caspase-3 plays a central role in the execution of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

Chen

Han

Zhan

et al. 2014

Cell Biochem Biophys

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This study was designed to investigate the apoptosis-inducing properties of Tegillarca granosa extract Haishengsu (HSS) in human hepatocellular carcinoma cell line BEL-7402. Proliferation inhibition of the human hepatocellular carcinoma BEL-7402 cells was determined by the MTT assay, and the cell viability was determined by trypan blue dye exclusion assay. Apoptosis of BEL-7402 cells was demonstrated by fluorescence microscope with flow cytometry with Annexin V-FITC/PI double staining and Hoechst 33258 staining. Western blot analysis and RT-PCR were used to determine the expression levels of Fas. Expressions of caspase-8 and caspase-3 were examined by caspase activity assay and western blot analysis. HSS inhibited the proliferation of human hepatocellular carcinoma BEL-7402 cells in a dose- and time-dependent manner. Our results showed HSS had positive effect on apoptosis through flow cytometry assay and fluorescence microscope. The expressions of Fas protein and mRNA were up-regulated following the treatment. Caspase-8 and caspase-3 were activated in the cells cultured with HSS. In conclusion, HSS induced apoptosis of human hepatocellular carcinoma BEL-7402 cells. The apoptosis was associated with the up-regulation of Fas and the activations of caspase-8 and caspase-3.

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Section: Discussionmentioning

confidence: 99%

Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

Chen

Han

Zhan

et al. 2014

Cell Biochem Biophys

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“…Activated caspase‐8 cleaves downstream substrates and transmits the death signals from receptors to members of the caspase family, including caspase‐3, caspase‐7 and caspase‐9, finally resulting in apoptosis. (Zielińska et al, 2008; Chen et al, 2009; Peng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…FasL, the Fas ligand, binds to Fas and induces the cross-linking of Fas molecules to the cell surface, thereby transferring apoptotic signals into cells and inducing apoptosis (Walczak and Krammer, 2000;Bullani et al, 2002;Nagata, 2004). Caspase-8 is a key promoter of Fasmediated apoptosis (Chen and Goeddel, 2002;Chen et al, 2008). Fas induces the polymerization of FADD (Fas-associated death domain), and FADD utilizes the DED (death effect domain) to recruit caspase-8, C-FLIP {cellular FLICE [FADD (Fas-associated death domain)-like interleukin 1b-converting enzyme]-inhibitory protein} and caspase-10 to form a DISC (death-inducing signalling complex).…”

Section: Introductionmentioning

confidence: 99%

PEG‐liposomal oxaliplatin induces apoptosis in human colorectal cancer cells via Fas/FasL and caspase‐8

Yang

Liu

2012

Cell Biology International

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Since cellular uptake of PEG [poly(ethylene glycol)]-liposomal L-OHP (oxaliplatin) induces bioactive changes in CRC (colorectal cancer), we have investigated its apoptotic effect and anticancer mechanism. Human CRC SW480 cells were treated with PEG-liposomal L-OHP and a caspase-8 inhibitor [Z-IETD-FMK (benzyloxycarbonyl-Ile-Glu-Thr-dl-Asp-fluoromethylketone)]. Apoptosis was measured by FCM (flow cytometry) and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling) assay. Expression of Fas/FasL and cytochrome c was detected using FCM and an immunofluorescence assay. Expression of caspase-8, Bid, caspase-9, caspase-7 and activated caspase-3 (P17) was examined by Western blot analyses. The results indicated that PEG-liposomal L-OHP (28 μg/ml L-OHP) induced marked apoptosis in SW480 cells compared with 28 μg/ml free L-OHP. The expression levels of Fas, FasL, cytochrome c, caspase-9, caspase-7 and activated caspase-3 proteins were up-regulated, with a corresponding increase in apoptosis; however, expression of caspase-8 and Bid were down-regulated as apoptosis increased. When cells were treated with Z-IETD-FMK, apoptosis was inhibited, but there was little impact on the expression of Fas, FasL, cytochrome c, Bid, caspase-9, caspase-7 and activated caspase-3. These findings indicate that PEG-liposomal L-OHP enhances the anticancer potency of the chemotherapeutic agent; moreover, Fas/FasL and caspase-8 signalling pathways play a key role in mediating PEG-liposomal L-OHP-induced apoptosis.

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“…Caspases are critical mediators of apoptosis [ 29 ]. Activation of caspase is responsible for multiple molecular and structural changes in apoptosis [ 30 ]. Caspase-3 is a potent effector of apoptosis in a variety of cells [ 31 ] and plays a central role in both death-receptor and mitochondria-mediated apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells

Juan

Feng

et al. 2011

J Exp Clin Cancer Res

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BackgroundOur study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.MethodsGastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [3H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.ResultsCell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.ConclusionsIT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines in vitro, and it provides an experimental basis for c-Met-targeted therapy towards in vivo testing.

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RGD-FasL Induces Apoptosis of Pituitary Adenoma Cells

Cited by 11 publications

References 39 publications

Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

PEG‐liposomal oxaliplatin induces apoptosis in human colorectal cancer cells via Fas/FasL and caspase‐8

Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells

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