Nontyphoid Salmonella carriage, serovar profile and antimicrobial resistance phenotypes in slaughter cattle

Abstract

Current nontyphoid Salmonella (NTS) carriage in 200 apparently healthy slaughter cattle by ISO 6579 standard bacteriology (ISO) was 1% (2/200) in carcass and fecal content, and 2% (4/200) in mesenterial lymph nodes. There was no isolation from liver, kidney, spleen, and gallbladder, with an overall prevalence of 4% (8/200). Real‐time PCR was in substantial agreement to ISO in confirming Salmonella‐suspect isolates (Relative Trueness: 93.33%). Predominant serovar was S. Enteritidis (50%) followed by S. Typhimurium (37.5%), and S. Albany (12.5%). Five and three of eight NTS isolates were susceptible (62.5%) and resistant (37.5%) to 18 antimicrobials, respectively. Only three S. Enteritidis isolates (37.5%) showed multidrug resistance to 2–3 of 7 antimicrobials (amikacin, cefotaxime, cefoxitin, gentamicin, norfloxacin, pefloxacin, and tobramycin). S. Enteritidis predominance over S. Typhimurium, first detection of S. Albany in cattle in Turkey, and sole resistance in mesenterial lymph node S. Enteritidis isolates highlights study findings. Practical applications Contaminated carcass and related material, for example, fecal content and mesenterial lymph nodes of apparently healthy slaughter cattle carrying nontyphoid Salmonella serovars still pose significant health risk to public in Turkey, where bovine meat covers the highest annual red meat consumption quota with high demand to edible offal. In this study, current predominance of S. Enteritidis, particularly in mesenterial lymph nodes, and the MDR pattern identified; the presence of S. Typhimurium as the second dominant and pansusceptible serovar; detection of S. Albany for the first time in cattle fecal content are new epidemiological findings. This data could be used in revising both bovine meat and offal's actual NTS status, and the control and prevention programs in our country and in the neighboring countries of interest.

Prevalence and Serotype Diversity of Salmonella in Apparently Healthy Cattle: Systematic Review and Meta-Analysis of Published Studies, 2000–2017

“…During this period, 6 full articles and three published abstracts representing 11 datasets were retrieved using the search engines (67–75). The majority of these studies were reported from Africa (67, 68, 70–74) except for two studies from Europe (69) and South America (75). Among the total of 5,868 cattle examined, 9.2% (554/6018), which is nearly equal to the pooled prevalence estimate, were reported to be positive for Salmonella species with different serotypes.…”

Linfonodos y carne molida de res como reservorios de Salmonella spp. de importancia en salud pública

“…Las frecuencias de positividad al patógeno aquí observadas (2.5 a 9.7 %), son inferiores a las reportadas en otros trabajos con carne molida (16-68 %) (4,5) y con linfonodos (50-94 %) (6,19,20) . No obstante, la variabilidad de este fenómeno entre zonas geográficas y épocas del año está bien documentada (20,21) . En general, el estudio confirma la importancia de los bovinos aparentemente sanos como reservorio de diversos serotipos de Salmonella de importancia epidemiológica.…”

Survival of Salmonella on Red Meat in Response to Dry Heat

“…Salmonella is a public health threat that is a key obstacle to safety in the red meat industry (1,3,10,66). A total of 31 Salmonella serotypes including S. Anatum, S. Heidelberg, S. Newport, S. Saintpaul and S. Typhimurium have been flagged as potential adulterants on meat (61).…”