Nontuberculous mycobacteria in gastrostomy fed patients with cystic fibrosis

Al-Momani, Hafez; Perry, Audrey; Jones, Rhiannon; Bourke, Stephen; Perry, John D.; Anderson, A. J.; Forrest, T.; Forrest, Ian; Griffin, Michael; Brodlie, Malcolm; Pearson, J. R. A.; Ward, Chris

doi:10.1038/srep46546

Cited by 12 publications

(17 citation statements)

References 29 publications

(43 reference statements)

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“…It is known for being responsible for a wide spectrum of diseases related to skin and soft tissues, peritonitis, central nervous system infections and bacteremia in humans [18]. Interestingly, presence of this NTM in gastric juice was recently reported among cystic fibrosis patients [19]. Here, using various techniques like culture, 16S rRNA gene sequencing, hsp65 gene sequencing, erm (41) gene PCR, acid-fast staining, immunohistochemistry and immunofluorescence we report that the infection of M .…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms

et al. 2019

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Development of gastric diseases such as gastritis, peptic ulcer and gastric cancer is often associated with several biotic and abiotic factors. Helicobacter pylori infection is such a well-known biotic factor. However, not all H. pylori-infected individuals develop gastric diseases and not all individuals with gastric diseases are infected with H. pylori. Therefore, it is possible that other gastric bacteria may contribute to the formation and progression of gastric disease. The aim of this study was to isolate prevalent gastric bacteria under microaerobic condition and identify them by 16S rRNA gene sequence analysis. Analysis of gastric biopsies showed infection of Mycobacterium abscessus (phylum Actinobacteria) to be highly prevalent in the stomachs of subjects included. Our data show that of 129 (67 male and 62 female) patients with gastric symptoms, 96 (51 male and 45 female) showed the presence of M. abscessus in stomach tissues. Infection of M. abscessus in gastric epithelium was further confirmed by imaging with acid fast staining, immunohistochemistry and immunofluorescence. Our imaging data strongly suggested that M. abscessus is an intracellular colonizer residing inside the gastric epithelial cells rather than in macrophages. Additionally, phylogenetic analysis of the mycobacterial hsp65 gene showed that the nearest match to the M. abscessus strains isolated from our study subjects is the M. abscessus strain ATCC 19977. Surprisingly, the subjects studied, the prevalence of M. abscessus infection in stomach is even higher than the prevalence of H. pylori infection. This, to the best of our knowledge, is the first study showing the colonization of M. abscessus in human gastric mucosa among patients with various gastric symptoms. This study could provide usher in a new opportunity to understand the role of less studied gastric bacteria in the development of gastric diseases.

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Section: Discussionmentioning

confidence: 99%

Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms

et al. 2019

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“…[83][84][85] A few studies have explored the relationship between NTM PD and gastroesophageal reflex disease (GERD). 86,87 NTM are readily found in the water we ingest and they are able to survive in a low pH environment. Additionally, in the lungs they persist in an intracellular compartment.…”

Section: Risk Factors Associated With Ntm Pulmonary Diseasementioning

confidence: 99%

Nontuberculous Mycobacteria

Kasperbauer

2021

Semin Respir Crit Care Med

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Nontuberculous mycobacteria (NTM) are ubiquitous in the environment and 193 species of NTM have been discovered thus far. NTM species vary in virulence from benign environmental organisms to difficult-to-treat human pathogens. Pulmonary infections remain the most common manifestation of NTM disease in humans and bronchiectasis continues to be a major risk factor for NTM pulmonary disease (NTM PD). This article will provide a useful introduction and framework for clinicians involved in the management of bronchiectasis and NTM. It includes an overview of the epidemiology, pathogenesis, diagnosis, and management of NTM PD. We will address the challenges faced in the diagnosis of NTM PD and the importance of subspeciation in guiding treatment and follow-up, especially in Mycobacterium abscessus infections. The treatment of both Mycobacterium avium complex and M. abscessus, the two most common NTM species known to cause disease, will be discussed in detail. Elements of the recent ATS/ERS/ESCMID/IDSA NTM guidelines published in 2020 will also be reviewed.

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“…It has also been reported that the increase of medical tourism among US citizens has led to the increase of NTM infections from cosmetic surgery performed in other countries [22]. Multi-drug resistant Mycobacterium abscessus complex has become a concern as the cause of infection in Cystic Fibrosis [23].…”

Section: Infection By M Tuberculosis Infection By Ntm Is Not Transmmentioning

confidence: 99%

The Increase of nontuberculous mycobacteria Infection: Emphasis on Epidemiology, Risk Factors and Diagnostic Tools

Suryawati¹,

Sarı²,

Saptawati³

2019

KLS

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Nontuberculous mycobacteria in gastrostomy fed patients with cystic fibrosis

Cited by 12 publications

References 29 publications

Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms

Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms

Nontuberculous Mycobacteria

The Increase of nontuberculous mycobacteria Infection: Emphasis on Epidemiology, Risk Factors and Diagnostic Tools

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