Nonthermal excitation effects mediated by sub-terahertz radiation on hydrogen exchange in ubiquitin

Tokunaga, Yuji; Tanaka, Masahito; Iida, Hitoshi; Kinoshita, Moto; Tojima, Yuya; Takeuchi, Koh; Imashimizu, Masahiko

doi:10.1016/j.bpj.2021.04.013

Cited by 6 publications

(8 citation statements)

References 60 publications

(84 reference statements)

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“…One successful method is selection by transition dipole direction using anisotropic terahertz microspectroscopy [73]. Other methods under consideration include isolating vibrations strongly coupled to a chromophore excitation [81] or determining the displacements induced by a specific THz frequency by hydrogen deuterium exchange mapping after high power THz excitation [82].…”

Section: Current and Future Challengesmentioning

confidence: 99%

The 2023 terahertz science and technology roadmap

Dhillon¹,

Vitiello²,

Linfield³

et al. 2023

J. Phys. D: Appl. Phys.

180

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Terahertz (THz) radiation encompasses a wide spectral range within the electromagnetic spectrum that extends from microwaves to the far infrared (100 GHz to ~30 THz). Within its frequency boundaries exist a broad variety of scientific disciplines that have presented, and continue to present, technical challenges to researchers. During the past 50 years, for instance, the demands of the scientific community have substantially evolved and with a need for advanced instrumentation to support radio astronomy, Earth observation, weather forecasting, security imaging, telecommunications, non-destructive device testing and much more. Furthermore, applications have required an emergence of technology from the laboratory environment to production-scale supply and in-the-field deployments ranging from harsh ground-based locations to deep space. In addressing these requirements, the research and development community has advanced related technology and bridged the transition between electronics and photonics that high frequency operation demands. The multidisciplinary nature of THz work was our stimulus for creating the 2017 THz Science and Technology Roadmap (S S Dhillon et al 2017 J. Phys. D: Appl. Phys. 50 043001). As one might envisage, though, there remains much to explore both scientifically and technically and the field has continued to develop and expand rapidly. It is timely, therefore, to revise our previous roadmap and in this 2023 version we both provide an update on key developments in established technical areas that have important scientific and public benefit, and highlight new and emerging areas that show particular promise. The developments that we describe thus span from fundamental scientific research, such as THz astronomy and the emergent area of THz quantum optics, to highly applied and commercially and societally impactful subjects that include 6G THz communications, medical imaging, and climate monitoring and prediction.

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Section: Current and Future Challengesmentioning

confidence: 99%

The 2023 terahertz science and technology roadmap

Dhillon¹,

Vitiello²,

Linfield³

et al. 2023

J. Phys. D: Appl. Phys.

180

View full text Add to dashboard Cite

show abstract

“…Moreover, local heating on cell membranes can induce alterations in biological reactions and functions such as permeability. Thus, the unusual heating of hydration water is likely to be a key factor for MW biological effects observed in the previous studies. − Furthermore, this study demonstrates that RM is a promising candidate for studying the role of hydration water in MW biological effects. This idea is due to the following advantages: realization of biomolecular reactions in RMs, − ,, control of the RM size, and the transparency of nonpolar solvents in RM solutions to MWs.…”

Section: Discussionmentioning

confidence: 57%

“…MWs on the high-frequency side are recently called subterahertz waves. MW irradiation alters the activities of cells and microorganisms, − the structures and their dynamics of proteins, − the rates of enzymatic reactions, , cell membrane permeability, , and so on. These outcomes are attributed to nonthermal MW effects.…”

Section: Introductionmentioning

confidence: 99%

Anomalously Large Heat Generation of Hydration Water under Microwave Irradiation

Murakami

2024

J. Phys. Chem. B

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Much attention has been paid to the biological effects of microwave irradiation. The hydration water surrounding a biomolecule is crucial in its biological reactions and functions. Therefore, it is important to know the response of hydration water to microwaves to understand their biological effects; however, the scarcity of studies about it often leads to speculations and debates about that effect. In this study, we have made realtime temperature measurements of reverse micellar solutions with their water droplet size from ∼2.3 to ∼9.5 nm using a waveguide system combined with a microwave generator at 2.45 GHz. The heat generated by water in reverse micelles has been observed to depend on their size. It is about 10 times larger than that of liquid water at their small sizes (<∼3.5 nm) and diminishes with further enlarging the size, approaching the water's value at their large sizes (∼10 nm). These results indicate that the heat generation behavior has an interfacial effect; specifically, the hydration water on the surfactant layer produces heat 10 times larger than bulk water. Moreover, the hydration number per surfactant molecule decreases in a core−shell model with increasing the reverse micelle size. These features are also reflected in the heat generation rate. Our findings may offer a new and fundamental perspective for studies on the biological effects of microwave irradiation.

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“…According to terahertz absorption spectra studies of protein solutes (validated against molecular dynamics simulations), the dynamic hydration shell around proteins can extend from ~ 14–22 Å, corresponding to at least five layers of water molecules ( 69 ). This ability for proteins to induce the structuring of their interfacial water layer (IWL) has been shown to play a critical role in the biological functions of proteins such as folding, enzymatic reactions, and protein-protein interactions ( 67 , 70 ). There is evidence that red-to-near-infrared (R–NIR) photons, and presumably, other wavelengths (for which bulk water is practically transparent) interact with the bound water, i.e., interfacial water layers.…”

Section: Discussionmentioning

confidence: 99%

Near-Infrared Photobiomodulation of Living Cells, Tubulin, and Microtubules In Vitro

Staelens

Gregorio

Kalra

et al. 2022

Front. Med. Technol.

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We report the results of experimental investigations involving photobiomodulation (PBM) of living cells, tubulin, and microtubules in buffer solutions exposed to near-infrared (NIR) light emitted from an 810 nm LED with a power density of 25 mW/cm2 pulsed at a frequency of 10 Hz. In the first group of experiments, we measured changes in the alternating current (AC) ionic conductivity in the 50–100 kHz range of HeLa and U251 cancer cell lines as living cells exposed to PBM for 60 min, and an increased resistance compared to the control cells was observed. In the second group of experiments, we investigated the stability and polymerization of microtubules under exposure to PBM. The protein buffer solution used was a mixture of Britton-Robinson buffer (BRB aka PEM) and microtubule cushion buffer. Exposure of Taxol-stabilized microtubules (~2 μM tubulin) to the LED for 120 min resulted in gradual disassembly of microtubules observed in fluorescence microscopy images. These results were compared to controls where microtubules remained stable. In the third group of experiments, we performed turbidity measurements throughout the tubulin polymerization process to quantify the rate and amount of polymerization for PBM-exposed tubulin vs. unexposed tubulin samples, using tubulin resuspended to final concentrations of ~ 22.7 μM and ~ 45.5 μM in the same buffer solution as before. Compared to the unexposed control samples, absorbance measurement results demonstrated a slower rate and reduced overall amount of polymerization in the less concentrated tubulin samples exposed to PBM for 30 min with the parameters mentioned above. Paradoxically, the opposite effect was observed in the 45.5 μM tubulin samples, demonstrating a remarkable increase in the polymerization rates and total polymer mass achieved after exposure to PBM. These results on the effects of PBM on living cells, tubulin, and microtubules are novel, further validating the modulating effects of PBM and contributing to designing more effective PBM parameters. Finally, potential consequences for the use of PBM in the context of neurodegenerative diseases are discussed.

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Nonthermal excitation effects mediated by sub-terahertz radiation on hydrogen exchange in ubiquitin

Cited by 6 publications

References 60 publications

The 2023 terahertz science and technology roadmap

The 2023 terahertz science and technology roadmap

Anomalously Large Heat Generation of Hydration Water under Microwave Irradiation

Near-Infrared Photobiomodulation of Living Cells, Tubulin, and Microtubules In Vitro

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