Nonsteroidal anti‐inflammatory drug associated right dorsal colitis in the horse

Davis, Jennifer L.

doi:10.1111/eve.12454

Cited by 26 publications

(82 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We propose that orally administered misoprostol may produce local, cAMP-mediated, anti-inflammatory effects on injured GI mucosa. While these data are promising, high doses of misoprostol have been associated with negative side effect in human, canine, and equine patients, which include abdominal discomfort and diarrhea ( 56 , 57 ). Additional studies utilizing e x- and in vivo equine inflammatory models are currently underway to investigate this hypothesis and assess the safety profile of this drug in relation to potential anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

et al. 2017

View full text Add to dashboard Cite

In many equine inflammatory disease states, neutrophil activities, such as adhesion, migration, and reactive oxygen species (ROS) production become dysregulated. Dysregulated neutrophil activation causes tissue damage in horses with asthma, colitis, laminitis, and gastric glandular disease. Non-steroidal anti-inflammatory drugs do not adequately inhibit neutrophil inflammatory functions and can lead to dangerous adverse effects. Therefore, novel therapies that target mechanisms of neutrophil-mediated tissue damage are needed. One potential neutrophil-targeting therapeutic is the PGE1 analog, misoprostol. Misoprostol is a gastroprotectant that induces intracellular formation of the secondary messenger molecule cyclic AMP (cAMP), which has been shown to have anti-inflammatory effects on neutrophils. Misoprostol is currently used in horses to treat NSAID-induced gastrointestinal injury; however, its effects on equine neutrophils have not been determined. We hypothesized that treatment of equine neutrophils with misoprostol would inhibit equine neutrophil adhesion, migration, and ROS production, in vitro. We tested this hypothesis using isolated equine peripheral blood neutrophils collected from 12 healthy adult teaching/research horses of mixed breed and gender. The effect of misoprostol treatment on adhesion, migration, and respiratory burst of equine neutrophils was evaluated via fluorescence-based adhesion and chemotaxis assays, and luminol-enhanced chemiluminescence, respectively. Neutrophils were pretreated with varying concentrations of misoprostol, vehicle, or appropriate functional inhibitory controls prior to stimulation with LTB4, CXCL8, PAF, lipopolysaccharide (LPS) or immune complex (IC). This study revealed that misoprostol pretreatment significantly inhibited LTB4-induced adhesion, LTB4-, CXCL8-, and PAF-induced chemotaxis, and LPS-, IC-, and PMA-induced ROS production in a concentration-dependent manner. This data indicate that misoprostol-targeting of E-prostanoid (EP) receptors potently inhibits equine neutrophil effector functions in vitro. Additional studies are indicated to further elucidate the role of EP receptors in regulating neutrophil function. Overall, our results suggest misoprostol may hold promise as a novel anti-inflammatory therapeutic in the horse.

show abstract

Section: Discussionmentioning

confidence: 99%

Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Indicando envolvimento de outros fatores que podem ser diminuídos seus impactos com a utilização de um modelo experimental. Porém, fica por esclarecer se as lesões induzidas por sobredoses de FBTZ são similares às induzidas em doenças clinicas 8 .…”

Section: Discussionunclassified

“…MEDICINA VETERINARIA Y ZOOTECNIA O diagnóstico das colites, especialmente da CDD, tem por base o histórico de administração de AINES, a observação de sinais clínicos, alterações hematológicas e bioquímicas, achados ultrassonográficos, laparoscópicos, cintilográficos e de laparotomia exploratória 7,8 . O prognóstico é reservado, mas isso pode ser atribuído em grande parte ao fato de o diagnóstico antemortem da CDD ser difícil e, muitas vezes, tardio 15 .…”

Section: Sobre Os Autoresunclassified

“…Como o acesso de toda a cavidade abdominal dos equinos é limitado 10 , o exame ultrassonográfico transabdominal (EUST) é um método dinâmico de valia considerável para a investigação da face parietal do abdome e de diversas vísceras 1 . O diagnóstico definitivo da CDD constitui um desafio, podendo permanecer à sombra de afecções como a síndrome da úlcera gástrica em equinos (EGUS), pois apresentam certos sinais clínicos e patofisiologia semelhantes e pelo fato de o estômago ser acessível por meios de diagnóstico por imagem direta (gastroscopia) 8,14,15 .…”

Section: Sobre Os Autoresunclassified

“…A Colite Dorsal Direita (CDD) em equinos é uma enteropatia caracterizada por inflamação, edema mural e ulcerações da mucosa 7,21,23 , normalmente associada a efeitos adversos de anti-inflamatório não esteroidais (AINEs), em particular a fenilbutazona (FBTZ) 13,22,23 . Apesar da patofisiologia da toxicose por FBTZ ainda não estar totalmente elucidada em equinos 8,21 , acredita-se que a mesma induz efeitos citotóxicos no trato gastrintestinal (TGI), como resultado da diminuição das concentrações de prostaglandinas (PGs) vasoativas e citoprotetoras 24 . O que desencadeia alteração no fluxo sanguíneo e inabilidade para restaurar-se a barreira da mucosa 19 .…”

Section: Introductionunclassified

See 2 more Smart Citations

Eficiência do exame ultrassonográfico transabdominal no auxílio ao diagnóstico precoce da colite dorsal direita induzida em equinos

Andrade¹,

Cassou

Aranzales

et al. 2016

Ces. Med. Vet. Zootec.

View full text Add to dashboard Cite

The Right Dorsal Colitis (CDD) is an enteropathy characterized by wall inflammation and edema and ulceration of the mucosa of the right dorsal colon in horses. Its occurrence is associated with prolonged use and/or overdoses of non-steroidal anti-inflammatory drugs (NSAIDs), especially phenylbutazone (FBTZ). Clinical abnormalities include anorexia, colic, hypoproteinemia, neutropenia, endotoxemia, diarrhea and weight loss. The ultrasound examination (EUS) is important in the diagnosis of abdominal abnormalities. Aiming to evaluate the efficiency of EUS in the early diagnosis of CDD, EUS were performed regularly in five horses (C1-C5) underwent protocol of induction experimental colitis. Five images per animal were taken on alternate days (D0-D14), between the 11th and 15th intercostal spaces in right side and, in each image, the wall thickness of the right dorsal colon was measured in millimeters (mm) at four different sites. There was no difference between D0 (3.4±0.55mm) and D3 (4.9 ± 1.79 mm), but in D5 (7.17 ± 1.28 mm), D7 (7.00 ± 1.68 mm) and D9 (6.71 ± 2.27 mm), there was significant increase in colon wall thickness in relation to D0 and D3 (p≤0.05). Since the onset of relevant clinical signs, such as diarrhea was evident in all animals only in D9, it was concluded that ultrasonography of the right dorsal colon is sensitive method as early diagnosis of CDD.

show abstract

Pathophysiology of Enteritis and Colitis

McKenzie

2017

The Equine Acute Abdomen

View full text Add to dashboard Cite

Nonsteroidal anti‐inflammatory drug associated right dorsal colitis in the horse

Cited by 26 publications

References 52 publications

Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

Eficiência do exame ultrassonográfico transabdominal no auxílio ao diagnóstico precoce da colite dorsal direita induzida em equinos

Pathophysiology of Enteritis and Colitis

Contact Info

Product

Resources

About