Nonspecific Cytotoxic Cell Antimicrobial Protein (NCAMP-1): A Novel Alarmin Ligand Identified in Zebrafish

Monette, Margaret M.; Evans, Donald L.; Krunkosky, Thomas M.; Camus, Alvin C.; Jaso-Friedmann, Liliana

doi:10.1371/journal.pone.0116576

Cited by 7 publications

(8 citation statements)

References 56 publications

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“…Sections were deparaffinized, rehydrated through graded ethanol, and stained using standard protocols. 20,21 In each group, ten animals were processed. For immunofluorescence at 72 hpf, the embryos were cryoprotected with 20% sucrose in 0.1 mol/L phosphate-buffered saline (pH 7.2) and frozen in optimal cutting temperature compound (Sakura Finetek, Torrance, CA, USA).…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Zhang¹,

He²,

Wang³

et al. 2016

IJN

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Copper oxide nanoparticles (CuO NPs) are used for a variety of purposes in a wide range of commercially available products. Some CuO NPs probably end up in the aquatic systems, thus raising concerns about aqueous exposure toxicity, and the impact of CuO NPs on liver development and neuronal differentiation remains unclear. In this study, particles were characterized using Fourier transform infrared spectra, scanning electron microscopy, and transmission electron microscopy. Zebrafish embryos were continuously exposed to CuO NPs from 4 hours postfertilization at concentrations of 50, 25, 12.5, 6.25, or 1 mg/L. The expression of gstp1 and cyp1a was examined by quantitative reverse transcription polymerase chain reaction. The expression of tumor necrosis factor alpha and superoxide dismutase 1 was examined by quantitative reverse transcription polymerase chain reaction and Western blotting. Liver development and retinal neurodifferentiation were analyzed by whole-mount in situ hybridization, hematoxylin–eosin staining, and immunohistochemistry, and a behavioral test was performed to track the movement of larvae. We show that exposure of CuO NPs at low doses has little effect on embryonic development. However, exposure to CuO NPs at concentrations of 12.5 mg/L or higher leads to abnormal phenotypes and induces an inflammatory response in a dose-dependent pattern. Moreover, exposure to CuO NPs at high doses results in an underdeveloped liver and a delay in retinal neurodifferentiation accompanied by reduced locomotor ability. Our data demonstrate that short-term exposure to CuO NPs at high doses shows hepatotoxicity and neurotoxicity in zebrafish embryos and larvae.

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Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Zhang¹,

He²,

Wang³

et al. 2016

IJN

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“…Sagittal slices were then prepared at a thickness of 5 µm. HE staining was performed using standard protocols [ 49 ]. Images of HE staining were captured with a BX51 microscope (Olympus).…”

Section: Methodsmentioning

confidence: 99%

Intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles prevents the progression of photoreceptor degeneration

Cao

Cheng

et al. 2023

J Nanobiotechnol

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Photoreceptor degeneration is one of the major causes of progressive blindness which lacks of curative treatment. GW2580, a highly selective inhibitor of colony-stimulating factor 1 receptor, has the protective potential on neurons; however, little was known about the application of GW2580 on photoreceptor degeneration. In this study, BV-2 and 661W cells coculture system was constructed to investigate the interaction between microglia and photoreceptors. GW2580 was loaded into zeolitic imidazolate framework-90-rhodamine B (ZIF-90-RhB) to synthesize a novel kind of nanoparticles, namely, ZIF-90-RhB-GW2580, through a one-step self-assembly approach. A photoreceptor degeneration model was generated by intense light exposure in zebrafish and ZIF-90-RhB-GW2580 nanoparticles were delivered by the intraocular injection. The results showed that in vitro GW2580 treatment promoted phenotypic transformation in microglia and led to the blockade of photoreceptor apoptosis. Following the intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles, the microglial proliferation and inflammatory response were significantly inhibited; moreover, the photoreceptors underwent alleviated injury with a recovery of retinal structure and visual function. In conclusion, the intraocular injection of ZIF-90-RhB-GW2580 at the early stage enables the precise delivery and sustained release of the GW2580, thus preventing the progression of photoreceptor degeneration. Graphical Abstract

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“…Nonspecific cytotoxic cell antimicrobial protein (NCAMP-1) is an H1X-like histone protein first identified in catfish and mouse [147,148] and later on in zebrafish [149]. In mammals, it is expressed in the membrane and in granule extracts from various populations of leukocytes, including natural killer (NK) cells and macrophages [148].…”

Section: Ncamp-1mentioning

confidence: 99%

“…In zebrafish, NCAMP-1 is detected in the coelomic cavity of various tissues, including kidney, liver, and intestine. In these tissues, it is present in the cytosol of both epithelial cells and various mononuclear cells, in particular nonspecific cytotoxic cells (NCCs), the equivalent of mammalian NK cells [149].…”

Section: Ncamp-1mentioning

confidence: 99%

Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish

Leiba

Özbilgiç

Hernández

et al. 2023

Biology

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Inflammation is a hallmark of the physiological response to aggressions. It is orchestrated by a plethora of molecules that detect the danger, signal intracellularly, and activate immune mechanisms to fight the threat. Understanding these processes at a level that allows to modulate their fate in a pathological context strongly relies on in vivo studies, as these can capture the complexity of the whole process and integrate the intricate interplay between the cellular and molecular actors of inflammation. Over the years, zebrafish has proven to be a well-recognized model to study immune responses linked to human physiopathology. We here provide a systematic review of the molecular effectors of inflammation known in this vertebrate and recapitulate their modes of action, as inferred from sterile or infection-based inflammatory models. We present a comprehensive analysis of their sequence, expression, and tissue distribution and summarize the tools that have been developed to study their function. We further highlight how these tools helped gain insights into the mechanisms of immune cell activation, induction, or resolution of inflammation, by uncovering downstream receptors and signaling pathways. These progresses pave the way for more refined models of inflammation, mimicking human diseases and enabling drug development using zebrafish models.

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Nonspecific Cytotoxic Cell Antimicrobial Protein (NCAMP-1): A Novel Alarmin Ligand Identified in Zebrafish

Cited by 7 publications

References 56 publications

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles prevents the progression of photoreceptor degeneration

Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish

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